BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1972008 1 BBa_K1972008 dszB with tac promoter induced by IPTG 2016-10-13T11:00:00Z 2016-10-19T07:24:24Z We use PCR to get this sequence from E.coli Bl21. T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and if there is protein gene with T7 promoter, it will be greatly expressed by the host cell. false false _2439_ 29948 29948 9 false Not yet. false Shuli Liang component2520880 1 BBa_B0015 component2520873 1 BBa_K1972005 annotation2520873 1 BBa_K1972005 range2520873 1 1 1170 annotation2520880 1 BBa_B0015 range2520880 1 1179 1307 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1972005 1 BBa_K1972005 dszB (a desulfinase gene), E.coli-optimized 2016-10-12T11:00:00Z 2016-10-13T09:45:10Z This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) DszB is a a desulfinase which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP) This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false No. false Shuli Liang annotation2500556 1 dszB-optimized range2500556 1 73 1170 annotation2500555 1 RBS range2500555 1 54 72 annotation2500554 1 tac promoter range2500554 1 1 53 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1972008_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1972005_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z