BBa_K1972010
type: ComponentDefinition
persistent identity: BBa K1972010
display id: BBa_K1972010
version: 1
was generated by: iGEM to SBOL conversion
description: dszBCAD with T7 promoter
created: 2016-09-15T11:00:00Z
modified: 2016-09-16T09:23:57Z
mutable provenance: 4 enzyme genes was systhsized respectively and we construted them to one cassette.
owned by: james
owned by: myers
mutable description: We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I712074
component: BBa_K1972000
component: BBa_K1972002
component: BBa_K1972001
component: BBa_J61100
component: BBa_B0012
component: BBa_K1972003
sequence annotation: BBa_I712074
sequence annotation: BBa_J61100
sequence annotation: BBa_K1972001
sequence annotation: BBa_K1972002
sequence annotation: BBa_K1972000
sequence annotation: BBa_K1972003
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1972010_sequence/1

BBa_I712074
type: Component
persistent identity: component2483673
display id: component2483673
version: 1
top level: BBa_K1972010
definition: BBa_I712074
access: public

BBa_K1972000
type: Component
persistent identity: component2483680
display id: component2483680
version: 1
top level: BBa_K1972010
definition: BBa_K1972000
access: public

BBa_K1972002
type: Component
persistent identity: component2483678
display id: component2483678
version: 1
top level: BBa_K1972010
definition: BBa_K1972002
access: public

BBa_K1972001
type: Component
persistent identity: component2483676
display id: component2483676
version: 1
top level: BBa_K1972010
definition: BBa_K1972001
access: public

BBa_J61100
type: Component
persistent identity: component2483674
display id: component2483674
version: 1
top level: BBa_K1972010
definition: BBa_J61100
access: public

BBa_B0012
type: Component
persistent identity: component2483683
display id: component2483683
version: 1
top level: BBa_K1972010
definition: BBa_B0012
access: public

BBa_K1972003
type: Component
persistent identity: component2483682
display id: component2483682
version: 1
top level: BBa_K1972010
definition: BBa_K1972003
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2483673
display id: annotation2483673
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483673/range2483673/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483673/range2483673/1
type: Range
persistent identity: range2483673
display id: range2483673
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2483674
display id: annotation2483674
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483674/range2483674/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483674/range2483674/1
type: Range
persistent identity: range2483674
display id: range2483674
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 55
end: 66
orientation: inline

BBa_K1972001
type: SequenceAnnotation
persistent identity: annotation2483676
display id: annotation2483676
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483676/range2483676/1
component: BBa_K1972001

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483676/range2483676/1
type: Range
persistent identity: range2483676
display id: range2483676
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 73
end: 1170
orientation: inline

BBa_K1972002
type: SequenceAnnotation
persistent identity: annotation2483678
display id: annotation2483678
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483678/range2483678/1
component: BBa_K1972002

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483678/range2483678/1
type: Range
persistent identity: range2483678
display id: range2483678
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 1177
end: 2430
orientation: inline

BBa_K1972000
type: SequenceAnnotation
persistent identity: annotation2483680
display id: annotation2483680
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483680/range2483680/1
component: BBa_K1972000

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483680/range2483680/1
type: Range
persistent identity: range2483680
display id: range2483680
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 2437
end: 3798
orientation: inline

BBa_K1972003
type: SequenceAnnotation
persistent identity: annotation2483682
display id: annotation2483682
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483682/range2483682/1
component: BBa_K1972003

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483682/range2483682/1
type: Range
persistent identity: range2483682
display id: range2483682
version: 1
top level: BBa_K1972010
direction: direction/reverse
start: 3805
end: 4383
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2483683
display id: annotation2483683
version: 1
top level: BBa_K1972010
location: https://synbiohub.org/public/igem/BBa_K1972010/annotation2483683/range2483683/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1972010/annotation2483683/range2483683/1
type: Range
persistent identity: range2483683
display id: range2483683
version: 1
top level: BBa_K1972010
direction: direction/forward
start: 4392
end: 4432
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_K1972000
type: ComponentDefinition
persistent identity: BBa K1972000
display id: BBa_K1972000
version: 1
was generated by: iGEM to SBOL conversion
description: dszA (a monooxygenase gene )
created: 2016-09-15T11:00:00Z
modified: 2016-10-09T02:51:39Z
mutable provenance: This single monooxygenase gene was synthesized and the sequence was found in NCBI (GenBank Accession number L37363.1)
owned by: james
owned by: myers
mutable description: DszA is a monoxygenase which can convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszA require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: dszA
sequence: https://synbiohub.org/public/igem/BBa_K1972000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2488747
display id: annotation2488747
version: 1
top level: BBa_K1972000
location: https://synbiohub.org/public/igem/BBa_K1972000/annotation2488747/range2488747/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1972000/annotation2488747/range2488747/1
type: Range
persistent identity: range2488747
display id: range2488747
version: 1
top level: BBa_K1972000
direction: direction/forward
start: 1
end: 21
orientation: inline

dszA
type: SequenceAnnotation
persistent identity: annotation2483626
display id: annotation2483626
version: 1
top level: BBa_K1972000
location: https://synbiohub.org/public/igem/BBa_K1972000/annotation2483626/range2483626/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1972000/annotation2483626/range2483626/1
type: Range
persistent identity: range2483626
display id: range2483626
version: 1
top level: BBa_K1972000
direction: direction/forward
start: 22
end: 1383
orientation: inline

BBa_K1972001
type: ComponentDefinition
persistent identity: BBa K1972001
display id: BBa_K1972001
version: 1
was generated by: iGEM to SBOL conversion
description: dszB (a desulfinase gene)
created: 2016-09-15T11:00:00Z
modified: 2016-10-09T02:59:34Z
mutable provenance: The desulfinase was found in Rhodococcus erythropolis IGTS8 and the gene was synthesized and the sequence was found in NCBI (GenBank Accession number L37363.1).
owned by: james
owned by: myers
mutable description: DszB is a a desulfinase which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP).
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This desulfinase can not work without cell system.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: dszB
sequence: https://synbiohub.org/public/igem/BBa_K1972001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2488759
display id: annotation2488759
version: 1
top level: BBa_K1972001
location: https://synbiohub.org/public/igem/BBa_K1972001/annotation2488759/range2488759/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1972001/annotation2488759/range2488759/1
type: Range
persistent identity: range2488759
display id: range2488759
version: 1
top level: BBa_K1972001
direction: direction/forward
start: 1
end: 19
orientation: inline

dszB
type: SequenceAnnotation
persistent identity: annotation2483627
display id: annotation2483627
version: 1
top level: BBa_K1972001
location: https://synbiohub.org/public/igem/BBa_K1972001/annotation2483627/range2483627/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1972001/annotation2483627/range2483627/1
type: Range
persistent identity: range2483627
display id: range2483627
version: 1
top level: BBa_K1972001
direction: direction/forward
start: 20
end: 1117
orientation: inline

BBa_K1972002
type: ComponentDefinition
persistent identity: BBa K1972002
display id: BBa_K1972002
version: 1
was generated by: iGEM to SBOL conversion
description: dszC (a monooxygenase gene )
created: 2016-09-15T11:00:00Z
modified: 2016-10-09T03:17:17Z
mutable provenance: The monooxygenase was found in Rhodococcus erythropolis IGTS8 and the gene was synthesized and the sequence was found in NCBI (GenBank Accession number L37363.1).
owned by: myers
owned by: james
mutable description: DszC is a monoxygenase which can not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszC require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: DszC require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
star: false
creator: Shuli Liang
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: dszC
sequence: https://synbiohub.org/public/igem/BBa_K1972002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2488780
display id: annotation2488780
version: 1
top level: BBa_K1972002
location: https://synbiohub.org/public/igem/BBa_K1972002/annotation2488780/range2488780/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1972002/annotation2488780/range2488780/1
type: Range
persistent identity: range2488780
display id: range2488780
version: 1
top level: BBa_K1972002
direction: direction/forward
start: 1
end: 20
orientation: inline

dszC
type: SequenceAnnotation
persistent identity: annotation2483628
display id: annotation2483628
version: 1
top level: BBa_K1972002
location: https://synbiohub.org/public/igem/BBa_K1972002/annotation2483628/range2483628/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1972002/annotation2483628/range2483628/1
type: Range
persistent identity: range2483628
display id: range2483628
version: 1
top level: BBa_K1972002
direction: direction/forward
start: 21
end: 1274
orientation: inline

BBa_K1972003
type: ComponentDefinition
persistent identity: BBa K1972003
display id: BBa_K1972003
version: 1
was generated by: iGEM to SBOL conversion
description: dszD (a flavinreductase)
created: 2016-09-15T11:00:00Z
modified: 2016-10-09T03:26:22Z
mutable provenance: This flavinreductase was found in Rhodococcus erythropolis IGTS8 and the gene was synthesized and the sequence was found in NCBI (GenBank Accession number AF048979.1).
owned by: james
owned by: myers
mutable description: DszA, DszB, DszC and dszD are required by the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This flavinreductase can be repaced by other flavinreductases from other bacteria.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: dszD
sequence: https://synbiohub.org/public/igem/BBa_K1972003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2488813
display id: annotation2488813
version: 1
top level: BBa_K1972003
location: https://synbiohub.org/public/igem/BBa_K1972003/annotation2488813/range2488813/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1972003/annotation2488813/range2488813/1
type: Range
persistent identity: range2488813
display id: range2488813
version: 1
top level: BBa_K1972003
direction: direction/forward
start: 1
end: 20
orientation: inline

dszD
type: SequenceAnnotation
persistent identity: annotation2483629
display id: annotation2483629
version: 1
top level: BBa_K1972003
location: https://synbiohub.org/public/igem/BBa_K1972003/annotation2483629/range2483629/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1972003/annotation2483629/range2483629/1
type: Range
persistent identity: range2483629
display id: range2483629
version: 1
top level: BBa_K1972003
direction: direction/forward
start: 21
end: 599
orientation: inline

https://synbiohub.org/public/igem/BBa_K1972010_sequence/1
type: Sequence
persistent identity: BBa K1972010 sequence
display id: BBa_K1972010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcacctacgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcggcggccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctccaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctgctcgggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccaggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972000_sequence/1
type: Sequence
persistent identity: BBa K1972000 sequence
display id: BBa_K1972000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caactgcaaggacaaccttcaatgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctccaggtaccgcgttcacctcagggtgagccggtgatcctccaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctccaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctccaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972001_sequence/1
type: Sequence
persistent identity: BBa K1972001 sequence
display id: BBa_K1972001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactgcaaggacaaccttcatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcacctacgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcggcggccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctccaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972002_sequence/1
type: Sequence
persistent identity: BBa K1972002 sequence
display id: BBa_K1972002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caactgcaaggacaaccttcatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctgctcgggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctccagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972003_sequence/1
type: Sequence
persistent identity: BBa K1972003 sequence
display id: BBa_K1972003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttccagccggagagttcccgatgtctgacaagccgaatgccgtttccagccacaccacccccgacgtccccgaagtagcggcgacgcccgagttgtccaccggcatctgcgccggtgactaccgcgctgcgcttcgccgccaccccgccggtgtcaccgtcgtgaccctcgattcgggtaccggcccggtgggtttcaccgccacctcgttctcgtccgtctccctcgagccgccgctcgtctcgttcaacatcgcggagacgtcgtcgagcatcaatgcactcaaggcagccgagtccttggtgatccaccttctcggcgaacatcagcagcatctggcccagcgctttgcgcgtagtgccgatcagcgttttgcagacgagtcactgtgggcagtgctcgacaccggggaaccggtgctgcacggcacccccagctggatgcgcgtcaaggtcgaccagctgatccctgtcggcgaccacacgctggtcatcggactcgtcacgcgggttcacgccgaagaagacgacgaatccgctgccgcgccgctgctctaccacgagggcaagtactaccgcccgactccgttaggtcaatag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z