LOCUS BBa_K1972013 6052 bp DNA linear 25-Nov-2024 DEFINITION dszBAC with tac promoter and dszD with lac promoter ACCESSION BBa_K1972013 VERSION BBa_K1972013.1 FEATURES Location/Qualifiers misc_feature 1..1770 /label=BBa_K1972005 misc_feature 1779..2561 /label=BBa_K1972004 misc_feature 2570..3841 /label= BBa_K1972006 promoter 3979..4021 /label=LacUV5 promoter misc_feature 3850..3978 /label=double teminator misc_feature 4022..4617 /label=BBa_K1972003 misc_feature 4618..4746 /label=double teminator promoter 4755..4832 /label=LacI promoter CDS 4956..5919 /label=LacI misc_feature 5924..6052 /label=double teminator ORIGIN 1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caaaactgca 61 aggacaacct tcatgacctc tcgcgttgat ccggccaatc cgggtagcga actggatagt 121 gccattcgcg ataccttaac ctatagtaat tgtccggttc cgaatgcact gttaaccgcc 181 tctgaaagcg gctttctgga tgcagcaggt attgaactgg atgtgctgag cggccagcag 241 ggtaccgttc attttaccta tgatcagccg gcatataccc gctttggcgg tgaaattccg 301 ccgctgctga gtgaaggcct gcgtgcccct ggtcgtaccc gcttactggg tattaccccg 361 ttactgggtc gccagggctt cttcgtgcgc gatgatagtc cgattaccgc agccgcagac 421 ctcgcaggtc gtcgtattgg tgtgagcgca tcagccattc gtattctgcg cggtcagctc 481 ggcgactatc tggaattaga tccgtggcgt cagaccttag ttgcattagg ctcatgggaa 541 gcacgtgcct tattacatac cctggaacat ggcgaattag gcgttgatga tgttgaactg 601 gttccgattt cttcaccggg tgtggatgtt ccggccgaac agttagaaga aagtgcaacc 661 gttaaaggtg ccgatctgtt tccggatgtt gcacgcggtc aggcagccgt gctggcaagt 721 ggcgatgtgg atgccctgta ttcttggctg ccgtgggcag gcgaattaca ggcaaccggc 781 gcacgtccgg ttgtggacct cggtttagat gaacgtaatg catatgcaag tgtttggacc 841 gtgtctagcg gtttagttcg ccagagacct ggcctggttc agcgtctggt tgatgccgcc 901 gttgatgcag gcttatgggc acgcgatcat tcagatgccg tgacctcact gcatgccgca 961 aatctgggtg tgtctaccgg cgcagttggt cagggctttg gcgcagattt tcagcagcgt 1021 ctggttccgc gtttagatca tgatgcctta gccctgttag aacgcaccca gcagtttctg 1081 ctgaccaaca acctgttaca ggaaccggtg gccttagatc agtgggccgc cccggaattt 1141 ttaaacaaca gcttaaatcg tcatcgctaa tactagagca actgcaagga caaccttcaa 1201 tgacccagca gcgccagatg caccttgcag gctttttcag cgcaggcaat gtgacccatg 1261 cacatggtgc atggcgtcat accgatgcct ctaatgattt tctgagcggt aaatattatc 1321 agcatattgc acgtacctta gaacgcggca aatttgatct gctgtttctg ccggatggtt 1381 tagccgttga agatagctat ggagacaatc tggataccgg cgtgggctta ggtggtcagg 1441 gtgcagttgc cttagaaccg gcaagcgttg tggcaaccat ggccgcagtg accgaacacc 1501 tgggtttagg tgcaaccatt agcgccacct attatccgcc gtatcatgtg gcacgtgtgt 1561 ttgccacctt agatcagctg agcggcggtc gtgtgtcttg gaatgttgtg acctcactga 1621 atgatgcaga agcacgtaat tttggtatta atcagcatct ggaacatgat gcacgctatg 1681 atcgcgccga tgaatttctg gaagccgtga aaaaactgtg gaactcttgg gatgaagatg 1741 ccctggtgct ggataaagca gccggcgtgt ttgccgatcc ggccaaagtt cattatgttg 1801 atcatcatgg cgaatggtta aatgtgcgcg gcccgttaca ggttccgcgt agtccgcagg 1861 gcgaaccggt tattttacag gcaggcctgt ctccgcgcgg tcgtcgcttt gccggtaaat 1921 gggcagaagc agtgttttca ctggccccga atctggaagt tatgcaggca acctatcagg 1981 gtattaaagc agaagttgat gccgcaggtc gcgatccgga tcagaccaaa atttttaccg 2041 ccgttatgcc ggtgctgggc gaaagtcagg cagtggccca ggaacgctta gaatatttga 2101 actcactggt tcatccggaa gttggcctgt ctaccctgag tagtcatacc ggtattaatc 2161 tggcagccta tccgttagat accccgatta aagatattct gcgcgacttg caggatcgta 2221 atgttccgac ccagctgcat atgtttgcag ccgccaccca tagtgaagaa ctgaccttag 2281 ccgaaatggg tcgtcgctat ggtaccaatg tgggctttgt tccgcagtgg gccggtaccg 2341 gcgaacagat tgccgatgaa ctgattcgtc attttgaagg cggcgccgca gatggcttta 2401 ttatttcacc ggcctttctg ccgggtagct atgatgaatt tgtggatcag gttgttccgg 2461 tgttacagga tcgtggctat tttcgcaccg aatatcaggg taataccctg cgtgatcatc 2521 tgggcttacg cgttccgcag ttacagggtc agccgagcta atactagaga ctgcaaggac 2581 aaccttcatg accctgtctc cggaaaaaca gcatgttcgt ccgcgtgatg ccgcagataa 2641 tgatccggtt gccgttgcac gtggcctggc agaaaaatgg cgcgccaccg ccgttgaacg 2701 tgatcgtgca ggcggttctg ccaccgcaga acgtgaagat ctgcgcgcct caggtctgct 2761 gtcactgctg gttccgcgtg aatatggcgg ctggggtgca gattggccga ccgcaattga 2821 agttgttcgc gaaattgcag ccgcagatgg ctcactgggt catctgtttg gctatcatct 2881 gaccaatgcc ccgatgattg aactgattgg ttcacaggaa caggaagaac atctgtatac 2941 ccagattgca cagaacaatt ggtggaccgg taatgcatca agcgaaaata actctcatgt 3001 gctggattgg aaagttcgcg caaccccgac cgaagatggc ggctatgtgc tgaatggtac 3061 caaacatttt tgtagcggcg ccaaaggtag cgacttgctg tttgtgtttg gcgttgttca 3121 ggatgatagt ccgcagcagg gcgcaattat tgccgcagca attccgacct ctcgcgccgg 3181 tgtgaccccg aatgatgatt gggcagccat tggcatgcgt cagaccgata gcggctctac 3241 cgattttcat aatgttaaag tggaaccgga tgaagtgctg ggtgcaccga atgcctttgt 3301 gttagccttt attcagtcag aacgcggtag tctgtttgcc ccgattgccc agttaatttt 3361 tgccaatgtg tatctaggta ttgcacatgg tgcattagat gcagcacgcg aatatacccg 3421 tacccaggca cgtccgtgga cccctgccgg tattcagcag gccaccgaag atccgtatac 3481 cattcgcagc tacggtgagt ttaccattgc attacagggc gccgatgcag ccgcacgcga 3541 agcagcccac ctcttacaga ccgtgtggga taaaggcgat gccctgaccc cggaagatcg 3601 tggtgaactg atggttaaag tgagcggcgt taaagcctta gcaaccaatg ccgccttaaa 3661 tatttctagt ggtgtgtttg aagttattgg tgcacgcggc acccatccgc gctatggctt 3721 tgatcgcttt tggcgtaatg ttcgtaccca tagcttacat gatccggtga gctacaagat 3781 tgccgatgtg ggcaaacata ccttaaatgg tcagtatccg attccgggct ttacctctta 3841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3961 ggcctttctg cgtttatagc ttccggctcg tataatgtgt ggaattgtga gcggataaca 4021 acagccggag agttcccgat gtctgacaag ccgaatgccg tttccagcca caccaccccc 4081 gacgtccccg aagtagcggc gacgcccgag ttgtccaccg gcatctgcgc cggtgactac 4141 cgcgctgcgc ttcgccgcca ccccgccggt gtcaccgtcg tgaccctcga ttcgggtacc 4201 ggcccggtgg gtttcaccgc cacctcgttc tcgtccgtct ccctcgagcc gccgctcgtc 4261 tcgttcaaca tcgcggagac gtcgtcgagc atcaatgcac tcaaggcagc cgagtccttg 4321 gtgatccacc ttctcggcga acatcagcag catctggccc agcgctttgc gcgtagtgcc 4381 gatcagcgtt ttgcagacga gtcactgtgg gcagtgctcg acaccgggga accggtgctg 4441 cacggcaccc ccagctggat gcgcgtcaag gtcgaccagc tgatccctgt cggcgaccac 4501 acgctggtca tcggactcgt cacgcgggtt cacgccgaag aagacgacga atccgctgcc 4561 gcgccgctgc tctaccacga gggcaagtac taccgcccga ctccgttagg tcaatagcca 4621 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 4681 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 4741 tttatatact agaggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc 4801 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg 4861 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 4921 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 4981 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 5041 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 5101 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 5161 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 5221 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 5281 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 5341 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 5401 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 5461 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 5521 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 5581 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg 5641 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 5701 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 5761 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 5821 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 5881 cacgacaggt ttcccgactg gaaagcgggc agtgatacta gagccaggca tcaaataaaa 5941 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 6001 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //