BBa_K1972013 1 BBa_K1972013 dszBAC with tac promoter and dszD with lac promoter 2016-10-13T11:00:00Z 2016-10-14T08:29:53Z Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) We constructed dszBCA genes with tac promoter and dszD gene with lac promoter choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway. Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false Not yet. false Shuli Liang annotation2506008 1 BBa_K1972004 range2506008 1 1779 2561 annotation2506076 1 double teminator range2506076 1 3850 3978 annotation2506104 1 LacI range2506104 1 4956 5919 annotation2505940 1 BBa_K1972005 range2505940 1 1 1770 annotation2506018 1 BBa_K1972006 range2506018 1 2570 3841 annotation2506092 1 BBa_K1972003 range2506092 1 4022 4617 annotation2506138 1 double teminator range2506138 1 5924 6052 annotation2506103 1 LacI promoter range2506103 1 4755 4832 annotation2506101 1 double teminator range2506101 1 4618 4746 annotation2506060 1 LacUV5 promoter range2506060 1 3979 4021 BBa_K1972013_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagcaactgcaaggacaaccttcaatgacccagcagcgccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctctaatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgccggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgccttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacctattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttgtgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgccgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggcgtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttccgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggcagaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgatgccgcaggtcgcgatccggatcagaccaaaatttttaccgccgttatgccggtgctgggcgaaagtcaggcagtggcccaggaacgcttagaatatttgaactcactggttcatccggaagttggcctgtctaccctgagtagtcataccggtattaatctggcagcctatccgttagataccccgattaaagatattctgcgcgacttgcaggatcgtaatgttccgacccagctgcatatgtttgcagccgccacccatagtgaagaactgaccttagccgaaatgggtcgtcgctatggtaccaatgtgggctttgttccgcagtgggccggtaccggcgaacagattgccgatgaactgattcgtcattttgaaggcggcgccgcagatggctttattatttcaccggcctttctgccgggtagctatgatgaatttgtggatcaggttgttccggtgttacaggatcgtggctattttcgcaccgaatatcagggtaataccctgcgtgatcatctgggcttacgcgttccgcagttacagggtcagccgagctaatactagagactgcaaggacaaccttcatgaccctgtctccggaaaaacagcatgttcgtccgcgtgatgccgcagataatgatccggttgccgttgcacgtggcctggcagaaaaatggcgcgccaccgccgttgaacgtgatcgtgcaggcggttctgccaccgcagaacgtgaagatctgcgcgcctcaggtctgctgtcactgctggttccgcgtgaatatggcggctggggtgcagattggccgaccgcaattgaagttgttcgcgaaattgcagccgcagatggctcactgggtcatctgtttggctatcatctgaccaatgccccgatgattgaactgattggttcacaggaacaggaagaacatctgtatacccagattgcacagaacaattggtggaccggtaatgcatcaagcgaaaataactctcatgtgctggattggaaagttcgcgcaaccccgaccgaagatggcggctatgtgctgaatggtaccaaacatttttgtagcggcgccaaaggtagcgacttgctgtttgtgtttggcgttgttcaggatgatagtccgcagcagggcgcaattattgccgcagcaattccgacctctcgcgccggtgtgaccccgaatgatgattgggcagccattggcatgcgtcagaccgatagcggctctaccgattttcataatgttaaagtggaaccggatgaagtgctgggtgcaccgaatgcctttgtgttagcctttattcagtcagaacgcggtagtctgtttgccccgattgcccagttaatttttgccaatgtgtatctaggtattgcacatggtgcattagatgcagcacgcgaatatacccgtacccaggcacgtccgtggacccctgccggtattcagcaggccaccgaagatccgtataccattcgcagctacggtgagtttaccattgcattacagggcgccgatgcagccgcacgcgaagcagcccacctcttacagaccgtgtgggataaaggcgatgccctgaccccggaagatcgtggtgaactgatggttaaagtgagcggcgttaaagccttagcaaccaatgccgccttaaatatttctagtggtgtgtttgaagttattggtgcacgcggcacccatccgcgctatggctttgatcgcttttggcgtaatgttcgtacccatagcttacatgatccggtgagctacaagattgccgatgtgggcaaacataccttaaatggtcagtatccgattccgggctttacctcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcttccggctcgtataatgtgtggaattgtgagcggataacaacagccggagagttcccgatgtctgacaagccgaatgccgtttccagccacaccacccccgacgtccccgaagtagcggcgacgcccgagttgtccaccggcatctgcgccggtgactaccgcgctgcgcttcgccgccaccccgccggtgtcaccgtcgtgaccctcgattcgggtaccggcccggtgggtttcaccgccacctcgttctcgtccgtctccctcgagccgccgctcgtctcgttcaacatcgcggagacgtcgtcgagcatcaatgcactcaaggcagccgagtccttggtgatccaccttctcggcgaacatcagcagcatctggcccagcgctttgcgcgtagtgccgatcagcgttttgcagacgagtcactgtgggcagtgctcgacaccggggaaccggtgctgcacggcacccccagctggatgcgcgtcaaggtcgaccagctgatccctgtcggcgaccacacgctggtcatcggactcgtcacgcgggttcacgccgaagaagacgacgaatccgctgccgcgccgctgctctaccacgagggcaagtactaccgcccgactccgttaggtcaatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z