BBa_K1972008 1 BBa_K1972008 dszB with tac promoter induced by IPTG 2016-10-13T11:00:00Z 2016-10-19T07:24:24Z We use PCR to get this sequence from E.coli Bl21. T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and if there is protein gene with T7 promoter, it will be greatly expressed by the host cell. false false _2439_ 29948 29948 9 false Not yet. false Shuli Liang component2520873 1 BBa_K1972005 component2520880 1 BBa_B0015 annotation2520873 1 BBa_K1972005 range2520873 1 1 1170 annotation2520880 1 BBa_B0015 range2520880 1 1179 1307 BBa_K1972005 1 BBa_K1972005 dszB (a desulfinase gene), E.coli-optimized 2016-10-12T11:00:00Z 2016-10-13T09:45:10Z This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) DszB is a a desulfinase which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP) This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false No. false Shuli Liang annotation2500554 1 tac promoter range2500554 1 1 53 annotation2500556 1 dszB-optimized range2500556 1 73 1170 annotation2500555 1 RBS range2500555 1 54 72 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1972014 1 BBa_K1972014 dszBBAC with tac promoter and dszD with lac promotor induced by IPTG 2016-10-13T11:00:00Z 2016-10-19T07:38:06Z Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) The T7 RNA polymerase controled system is PCRed from E.coli Bl21. We constructed dszBCAD genes with T7 promoter and IPTG controled system and choose E.coli as our host cell.T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and dszBCAD genes with T7 promoter will be greatly expressed by the host cell. The four enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway. Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false Not yet. false Shuli Liang component2520908 1 BBa_K1972005 component2520907 1 BBa_K1972008 component2520918 1 BBa_K1972013 annotation2520908 1 BBa_K1972005 range2520908 1 1316 3085 annotation2520907 1 BBa_K1972008 range2520907 1 1 1307 annotation2520918 1 BBa_K1972013 range2520918 1 1316 7367 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1972013 1 BBa_K1972013 dszBAC with tac promoter and dszD with lac promoter 2016-10-13T11:00:00Z 2016-10-14T08:29:53Z Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) We constructed dszBCA genes with tac promoter and dszD gene with lac promoter choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway. Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) false false _2439_ 29948 29948 9 false Not yet. false Shuli Liang annotation2506138 1 double teminator range2506138 1 5924 6052 annotation2505940 1 BBa_K1972005 range2505940 1 1 1770 annotation2506092 1 BBa_K1972003 range2506092 1 4022 4617 annotation2506076 1 double teminator range2506076 1 3850 3978 annotation2506060 1 LacUV5 promoter range2506060 1 3979 4021 annotation2506018 1 BBa_K1972006 range2506018 1 2570 3841 annotation2506008 1 BBa_K1972004 range2506008 1 1779 2561 annotation2506103 1 LacI promoter range2506103 1 4755 4832 annotation2506101 1 double teminator range2506101 1 4618 4746 annotation2506104 1 LacI range2506104 1 4956 5919 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1972008_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1972005_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaa BBa_K1972013_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagcaactgcaaggacaaccttcaatgacccagcagcgccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctctaatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgccggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgccttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacctattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttgtgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgccgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggcgtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttccgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggcagaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgatgccgcaggtcgcgatccggatcagaccaaaatttttaccgccgttatgccggtgctgggcgaaagtcaggcagtggcccaggaacgcttagaatatttgaactcactggttcatccggaagttggcctgtctaccctgagtagtcataccggtattaatctggcagcctatccgttagataccccgattaaagatattctgcgcgacttgcaggatcgtaatgttccgacccagctgcatatgtttgcagccgccacccatagtgaagaactgaccttagccgaaatgggtcgtcgctatggtaccaatgtgggctttgttccgcagtgggccggtaccggcgaacagattgccgatgaactgattcgtcattttgaaggcggcgccgcagatggctttattatttcaccggcctttctgccgggtagctatgatgaatttgtggatcaggttgttccggtgttacaggatcgtggctattttcgcaccgaatatcagggtaataccctgcgtgatcatctgggcttacgcgttccgcagttacagggtcagccgagctaatactagagactgcaaggacaaccttcatgaccctgtctccggaaaaacagcatgttcgtccgcgtgatgccgcagataatgatccggttgccgttgcacgtggcctggcagaaaaatggcgcgccaccgccgttgaacgtgatcgtgcaggcggttctgccaccgcagaacgtgaagatctgcgcgcctcaggtctgctgtcactgctggttccgcgtgaatatggcggctggggtgcagattggccgaccgcaattgaagttgttcgcgaaattgcagccgcagatggctcactgggtcatctgtttggctatcatctgaccaatgccccgatgattgaactgattggttcacaggaacaggaagaacatctgtatacccagattgcacagaacaattggtggaccggtaatgcatcaagcgaaaataactctcatgtgctggattggaaagttcgcgcaaccccgaccgaagatggcggctatgtgctgaatggtaccaaacatttttgtagcggcgccaaaggtagcgacttgctgtttgtgtttggcgttgttcaggatgatagtccgcagcagggcgcaattattgccgcagcaattccgacctctcgcgccggtgtgaccccgaatgatgattgggcagccattggcatgcgtcagaccgatagcggctctaccgattttcataatgttaaagtggaaccggatgaagtgctgggtgcaccgaatgcctttgtgttagcctttattcagtcagaacgcggtagtctgtttgccccgattgcccagttaatttttgccaatgtgtatctaggtattgcacatggtgcattagatgcagcacgcgaatatacccgtacccaggcacgtccgtggacccctgccggtattcagcaggccaccgaagatccgtataccattcgcagctacggtgagtttaccattgcattacagggcgccgatgcagccgcacgcgaagcagcccacctcttacagaccgtgtgggataaaggcgatgccctgaccccggaagatcgtggtgaactgatggttaaagtgagcggcgttaaagccttagcaaccaatgccgccttaaatatttctagtggtgtgtttgaagttattggtgcacgcggcacccatccgcgctatggctttgatcgcttttggcgtaatgttcgtacccatagcttacatgatccggtgagctacaagattgccgatgtgggcaaacataccttaaatggtcagtatccgattccgggctttacctcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcttccggctcgtataatgtgtggaattgtgagcggataacaacagccggagagttcccgatgtctgacaagccgaatgccgtttccagccacaccacccccgacgtccccgaagtagcggcgacgcccgagttgtccaccggcatctgcgccggtgactaccgcgctgcgcttcgccgccaccccgccggtgtcaccgtcgtgaccctcgattcgggtaccggcccggtgggtttcaccgccacctcgttctcgtccgtctccctcgagccgccgctcgtctcgttcaacatcgcggagacgtcgtcgagcatcaatgcactcaaggcagccgagtccttggtgatccaccttctcggcgaacatcagcagcatctggcccagcgctttgcgcgtagtgccgatcagcgttttgcagacgagtcactgtgggcagtgctcgacaccggggaaccggtgctgcacggcacccccagctggatgcgcgtcaaggtcgaccagctgatccctgtcggcgaccacacgctggtcatcggactcgtcacgcgggttcacgccgaagaagacgacgaatccgctgccgcgccgctgctctaccacgagggcaagtactaccgcccgactccgttaggtcaatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1972014_sequence 1 ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagcaactgcaaggacaaccttcaatgacccagcagcgccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctctaatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgccggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgccttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacctattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttgtgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgccgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggcgtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttccgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggcagaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgatgccgcaggtcgcgatccggatcagaccaaaatttttaccgccgttatgccggtgctgggcgaaagtcaggcagtggcccaggaacgcttagaatatttgaactcactggttcatccggaagttggcctgtctaccctgagtagtcataccggtattaatctggcagcctatccgttagataccccgattaaagatattctgcgcgacttgcaggatcgtaatgttccgacccagctgcatatgtttgcagccgccacccatagtgaagaactgaccttagccgaaatgggtcgtcgctatggtaccaatgtgggctttgttccgcagtgggccggtaccggcgaacagattgccgatgaactgattcgtcattttgaaggcggcgccgcagatggctttattatttcaccggcctttctgccgggtagctatgatgaatttgtggatcaggttgttccggtgttacaggatcgtggctattttcgcaccgaatatcagggtaataccctgcgtgatcatctgggcttacgcgttccgcagttacagggtcagccgagctaatactagagactgcaaggacaaccttcatgaccctgtctccggaaaaacagcatgttcgtccgcgtgatgccgcagataatgatccggttgccgttgcacgtggcctggcagaaaaatggcgcgccaccgccgttgaacgtgatcgtgcaggcggttctgccaccgcagaacgtgaagatctgcgcgcctcaggtctgctgtcactgctggttccgcgtgaatatggcggctggggtgcagattggccgaccgcaattgaagttgttcgcgaaattgcagccgcagatggctcactgggtcatctgtttggctatcatctgaccaatgccccgatgattgaactgattggttcacaggaacaggaagaacatctgtatacccagattgcacagaacaattggtggaccggtaatgcatcaagcgaaaataactctcatgtgctggattggaaagttcgcgcaaccccgaccgaagatggcggctatgtgctgaatggtaccaaacatttttgtagcggcgccaaaggtagcgacttgctgtttgtgtttggcgttgttcaggatgatagtccgcagcagggcgcaattattgccgcagcaattccgacctctcgcgccggtgtgaccccgaatgatgattgggcagccattggcatgcgtcagaccgatagcggctctaccgattttcataatgttaaagtggaaccggatgaagtgctgggtgcaccgaatgcctttgtgttagcctttattcagtcagaacgcggtagtctgtttgccccgattgcccagttaatttttgccaatgtgtatctaggtattgcacatggtgcattagatgcagcacgcgaatatacccgtacccaggcacgtccgtggacccctgccggtattcagcaggccaccgaagatccgtataccattcgcagctacggtgagtttaccattgcattacagggcgccgatgcagccgcacgcgaagcagcccacctcttacagaccgtgtgggataaaggcgatgccctgaccccggaagatcgtggtgaactgatggttaaagtgagcggcgttaaagccttagcaaccaatgccgccttaaatatttctagtggtgtgtttgaagttattggtgcacgcggcacccatccgcgctatggctttgatcgcttttggcgtaatgttcgtacccatagcttacatgatccggtgagctacaagattgccgatgtgggcaaacataccttaaatggtcagtatccgattccgggctttacctcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcttccggctcgtataatgtgtggaattgtgagcggataacaacagccggagagttcccgatgtctgacaagccgaatgccgtttccagccacaccacccccgacgtccccgaagtagcggcgacgcccgagttgtccaccggcatctgcgccggtgactaccgcgctgcgcttcgccgccaccccgccggtgtcaccgtcgtgaccctcgattcgggtaccggcccggtgggtttcaccgccacctcgttctcgtccgtctccctcgagccgccgctcgtctcgttcaacatcgcggagacgtcgtcgagcatcaatgcactcaaggcagccgagtccttggtgatccaccttctcggcgaacatcagcagcatctggcccagcgctttgcgcgtagtgccgatcagcgttttgcagacgagtcactgtgggcagtgctcgacaccggggaaccggtgctgcacggcacccccagctggatgcgcgtcaaggtcgaccagctgatccctgtcggcgaccacacgctggtcatcggactcgtcacgcgggttcacgccgaagaagacgacgaatccgctgccgcgccgctgctctaccacgagggcaagtactaccgcccgactccgttaggtcaatagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z