BBa_K1972014
type: ComponentDefinition
persistent identity: BBa K1972014
display id: BBa_K1972014
version: 1
was generated by: iGEM to SBOL conversion
description: dszBBAC with tac promoter and dszD with lac promotor induced by IPTG
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T07:38:06Z
mutable provenance: Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) The T7 RNA polymerase controled system is PCRed from E.coli Bl21.
owned by: james
owned by: myers
mutable description: We constructed dszBCAD genes with T7 promoter and IPTG controled system and choose E.coli as our host cell.T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and dszBCAD genes with T7 promoter will be greatly expressed by the host cell. The four enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway. Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
discontinued: false
dominant: false
experience: experience/None
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Not yet.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1972013
component: BBa_K1972005
component: BBa_K1972008
sequence annotation: BBa_K1972008
sequence annotation: BBa_K1972013
sequence annotation: BBa_K1972005
sequence: https://synbiohub.org/public/igem/BBa_K1972014_sequence/1

BBa_K1972013
type: Component
persistent identity: component2520918
display id: component2520918
version: 1
top level: BBa_K1972014
definition: BBa_K1972013
access: public

BBa_K1972005
type: Component
persistent identity: component2520908
display id: component2520908
version: 1
top level: BBa_K1972014
definition: BBa_K1972005
access: public

BBa_K1972008
type: Component
persistent identity: component2520907
display id: component2520907
version: 1
top level: BBa_K1972014
definition: BBa_K1972008
access: public

BBa_K1972008
type: SequenceAnnotation
persistent identity: annotation2520907
display id: annotation2520907
version: 1
top level: BBa_K1972014
location: https://synbiohub.org/public/igem/BBa_K1972014/annotation2520907/range2520907/1
component: BBa_K1972008

https://synbiohub.org/public/igem/BBa_K1972014/annotation2520907/range2520907/1
type: Range
persistent identity: range2520907
display id: range2520907
version: 1
top level: BBa_K1972014
direction: direction/reverse
start: 1
end: 1307
orientation: inline

BBa_K1972013
type: SequenceAnnotation
persistent identity: annotation2520918
display id: annotation2520918
version: 1
top level: BBa_K1972014
location: https://synbiohub.org/public/igem/BBa_K1972014/annotation2520918/range2520918/1
component: BBa_K1972013

https://synbiohub.org/public/igem/BBa_K1972014/annotation2520918/range2520918/1
type: Range
persistent identity: range2520918
display id: range2520918
version: 1
top level: BBa_K1972014
direction: direction/reverse
start: 1316
end: 7367
orientation: inline

BBa_K1972005
type: SequenceAnnotation
persistent identity: annotation2520908
display id: annotation2520908
version: 1
top level: BBa_K1972014
location: https://synbiohub.org/public/igem/BBa_K1972014/annotation2520908/range2520908/1
component: BBa_K1972005

https://synbiohub.org/public/igem/BBa_K1972014/annotation2520908/range2520908/1
type: Range
persistent identity: range2520908
display id: range2520908
version: 1
top level: BBa_K1972014
direction: direction/forward
start: 1316
end: 3085
orientation: inline

BBa_K1972005
type: ComponentDefinition
persistent identity: BBa K1972005
display id: BBa_K1972005
version: 1
was generated by: iGEM to SBOL conversion
description: dszB (a desulfinase gene), E.coli-optimized
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T09:45:10Z
mutable provenance: This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1)
owned by: james
owned by: myers
mutable description: DszB is a a desulfinase which can convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP) This single monooxygenase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1)
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tac promoter
sequence annotation: RBS
sequence annotation: dszB-optimized
sequence: https://synbiohub.org/public/igem/BBa_K1972005_sequence/1

tac promoter
type: SequenceAnnotation
persistent identity: annotation2500554
display id: annotation2500554
version: 1
top level: BBa_K1972005
location: https://synbiohub.org/public/igem/BBa_K1972005/annotation2500554/range2500554/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1972005/annotation2500554/range2500554/1
type: Range
persistent identity: range2500554
display id: range2500554
version: 1
top level: BBa_K1972005
direction: direction/forward
start: 1
end: 53
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2500555
display id: annotation2500555
version: 1
top level: BBa_K1972005
location: https://synbiohub.org/public/igem/BBa_K1972005/annotation2500555/range2500555/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1972005/annotation2500555/range2500555/1
type: Range
persistent identity: range2500555
display id: range2500555
version: 1
top level: BBa_K1972005
direction: direction/forward
start: 54
end: 72
orientation: inline

dszB-optimized
type: SequenceAnnotation
persistent identity: annotation2500556
display id: annotation2500556
version: 1
top level: BBa_K1972005
location: https://synbiohub.org/public/igem/BBa_K1972005/annotation2500556/range2500556/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1972005/annotation2500556/range2500556/1
type: Range
persistent identity: range2500556
display id: range2500556
version: 1
top level: BBa_K1972005
direction: direction/forward
start: 73
end: 1170
orientation: inline

BBa_K1972008
type: ComponentDefinition
persistent identity: BBa K1972008
display id: BBa_K1972008
version: 1
was generated by: iGEM to SBOL conversion
description: dszB with tac promoter induced by IPTG
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T07:24:24Z
mutable provenance: We use PCR to get this sequence from E.coli Bl21.
owned by: james
owned by: myers
mutable description: T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and if there is protein gene with T7 promoter, it will be greatly expressed by the host cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Not yet.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_K1972005
sequence annotation: BBa_K1972005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1972008_sequence/1

BBa_B0015
type: Component
persistent identity: component2520880
display id: component2520880
version: 1
top level: BBa_K1972008
definition: BBa_B0015
access: public

BBa_K1972005
type: Component
persistent identity: component2520873
display id: component2520873
version: 1
top level: BBa_K1972008
definition: BBa_K1972005
access: public

BBa_K1972005
type: SequenceAnnotation
persistent identity: annotation2520873
display id: annotation2520873
version: 1
top level: BBa_K1972008
location: https://synbiohub.org/public/igem/BBa_K1972008/annotation2520873/range2520873/1
component: BBa_K1972005

https://synbiohub.org/public/igem/BBa_K1972008/annotation2520873/range2520873/1
type: Range
persistent identity: range2520873
display id: range2520873
version: 1
top level: BBa_K1972008
direction: direction/reverse
start: 1
end: 1170
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520880
display id: annotation2520880
version: 1
top level: BBa_K1972008
location: https://synbiohub.org/public/igem/BBa_K1972008/annotation2520880/range2520880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1972008/annotation2520880/range2520880/1
type: Range
persistent identity: range2520880
display id: range2520880
version: 1
top level: BBa_K1972008
direction: direction/reverse
start: 1179
end: 1307
orientation: inline

BBa_K1972013
type: ComponentDefinition
persistent identity: BBa K1972013
display id: BBa_K1972013
version: 1
was generated by: iGEM to SBOL conversion
description: dszBAC with tac promoter and dszD with lac promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T08:29:53Z
mutable provenance: Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
owned by: james
owned by: myers
mutable description: We constructed dszBCA genes with tac promoter and dszD gene with lac promoter choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway. Each single enzyme gene was synthesized by Generay with E.coli optimized and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
discontinued: false
dominant: false
group u list: _2439_
m user id: 29948
owner id: 29948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Not yet.
star: false
creator: Shuli Liang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1972005
sequence annotation: BBa_K1972004
sequence annotation: BBa_K1972006
sequence annotation: double teminator
sequence annotation: LacUV5 promoter
sequence annotation: BBa_K1972003
sequence annotation: LacI promoter
sequence annotation: LacI
sequence: https://synbiohub.org/public/igem/BBa_K1972013_sequence/1

BBa_K1972005
type: SequenceAnnotation
persistent identity: annotation2505940
display id: annotation2505940
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2505940/range2505940/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1972013/annotation2505940/range2505940/1
type: Range
persistent identity: range2505940
display id: range2505940
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 1
end: 1770
orientation: inline

BBa_K1972004
type: SequenceAnnotation
persistent identity: annotation2506008
display id: annotation2506008
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506008/range2506008/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506008/range2506008/1
type: Range
persistent identity: range2506008
display id: range2506008
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 1779
end: 2561
orientation: inline

BBa_K1972006
type: SequenceAnnotation
persistent identity: annotation2506018
display id: annotation2506018
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506018/range2506018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506018/range2506018/1
type: Range
persistent identity: range2506018
display id: range2506018
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 2570
end: 3841
orientation: inline

double teminator
type: SequenceAnnotation
persistent identity: annotation2506076
display id: annotation2506076
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506076/range2506076/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506076/range2506076/1
type: Range
persistent identity: range2506076
display id: range2506076
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 3850
end: 3978
orientation: inline

LacUV5 promoter
type: SequenceAnnotation
persistent identity: annotation2506060
display id: annotation2506060
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506060/range2506060/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506060/range2506060/1
type: Range
persistent identity: range2506060
display id: range2506060
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 3979
end: 4021
orientation: inline

BBa_K1972003
type: SequenceAnnotation
persistent identity: annotation2506092
display id: annotation2506092
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506092/range2506092/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506092/range2506092/1
type: Range
persistent identity: range2506092
display id: range2506092
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 4022
end: 4617
orientation: inline

double teminator
type: SequenceAnnotation
persistent identity: annotation2506101
display id: annotation2506101
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506101/range2506101/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506101/range2506101/1
type: Range
persistent identity: range2506101
display id: range2506101
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 4618
end: 4746
orientation: inline

LacI promoter
type: SequenceAnnotation
persistent identity: annotation2506103
display id: annotation2506103
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506103/range2506103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506103/range2506103/1
type: Range
persistent identity: range2506103
display id: range2506103
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 4755
end: 4832
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2506104
display id: annotation2506104
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506104/range2506104/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506104/range2506104/1
type: Range
persistent identity: range2506104
display id: range2506104
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 4956
end: 5919
orientation: inline

double teminator
type: SequenceAnnotation
persistent identity: annotation2506138
display id: annotation2506138
version: 1
top level: BBa_K1972013
location: https://synbiohub.org/public/igem/BBa_K1972013/annotation2506138/range2506138/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1972013/annotation2506138/range2506138/1
type: Range
persistent identity: range2506138
display id: range2506138
version: 1
top level: BBa_K1972013
direction: direction/forward
start: 5924
end: 6052
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1972014_sequence/1
type: Sequence
persistent identity: BBa K1972014 sequence
display id: BBa_K1972014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972005_sequence/1
type: Sequence
persistent identity: BBa K1972005 sequence
display id: BBa_K1972005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972008_sequence/1
type: Sequence
persistent identity: BBa K1972008 sequence
display id: BBa_K1972008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1972013_sequence/1
type: Sequence
persistent identity: BBa K1972013 sequence
display id: BBa_K1972013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaaaactgcaaggacaaccttcatgacctctcgcgttgatccggccaatccgggtagcgaactggatagtgccattcgcgataccttaacctatagtaattgtccggttccgaatgcactgttaaccgcctctgaaagcggctttctggatgcagcaggtattgaactggatgtgctgagcggccagcagggtaccgttcattttacctatgatcagccggcatatacccgctttggcggtgaaattccgccgctgctgagtgaaggcctgcgtgcccctggtcgtacccgcttactgggtattaccccgttactgggtcgccagggcttcttcgtgcgcgatgatagtccgattaccgcagccgcagacctcgcaggtcgtcgtattggtgtgagcgcatcagccattcgtattctgcgcggtcagctcggcgactatctggaattagatccgtggcgtcagaccttagttgcattaggctcatgggaagcacgtgccttattacataccctggaacatggcgaattaggcgttgatgatgttgaactggttccgatttcttcaccgggtgtggatgttccggccgaacagttagaagaaagtgcaaccgttaaaggtgccgatctgtttccggatgttgcacgcggtcaggcagccgtgctggcaagtggcgatgtggatgccctgtattcttggctgccgtgggcaggcgaattacaggcaaccggcgcacgtccggttgtggacctcggtttagatgaacgtaatgcatatgcaagtgtttggaccgtgtctagcggtttagttcgccagagacctggcctggttcagcgtctggttgatgccgccgttgatgcaggcttatgggcacgcgatcattcagatgccgtgacctcactgcatgccgcaaatctgggtgtgtctaccggcgcagttggtcagggctttggcgcagattttcagcagcgtctggttccgcgtttagatcatgatgccttagccctgttagaacgcacccagcagtttctgctgaccaacaacctgttacaggaaccggtggccttagatcagtgggccgccccggaatttttaaacaacagcttaaatcgtcatcgctaatactagagcaactgcaaggacaaccttcaatgacccagcagcgccagatgcaccttgcaggctttttcagcgcaggcaatgtgacccatgcacatggtgcatggcgtcataccgatgcctctaatgattttctgagcggtaaatattatcagcatattgcacgtaccttagaacgcggcaaatttgatctgctgtttctgccggatggtttagccgttgaagatagctatggagacaatctggataccggcgtgggcttaggtggtcagggtgcagttgccttagaaccggcaagcgttgtggcaaccatggccgcagtgaccgaacacctgggtttaggtgcaaccattagcgccacctattatccgccgtatcatgtggcacgtgtgtttgccaccttagatcagctgagcggcggtcgtgtgtcttggaatgttgtgacctcactgaatgatgcagaagcacgtaattttggtattaatcagcatctggaacatgatgcacgctatgatcgcgccgatgaatttctggaagccgtgaaaaaactgtggaactcttgggatgaagatgccctggtgctggataaagcagccggcgtgtttgccgatccggccaaagttcattatgttgatcatcatggcgaatggttaaatgtgcgcggcccgttacaggttccgcgtagtccgcagggcgaaccggttattttacaggcaggcctgtctccgcgcggtcgtcgctttgccggtaaatgggcagaagcagtgttttcactggccccgaatctggaagttatgcaggcaacctatcagggtattaaagcagaagttgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z