BBa_K1973000 1 BBa_K1973000 lapG 2016-10-08T11:00:00Z 2016-10-12T05:16:10Z Obtained by PCR from the genome of Pseudomonas putida KT2442. Codifies the LapG protein from Pseudomonas putida KT2442, a periplasmic protease that participates in the biofilm dispersion process by cleaving adhesion proteins such as LapA. false false _2440_ 30017 30003 9 false We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts false Laura Claret Fern??ndez annotation2489353 1 lapG range2489353 1 19 663 annotation2489352 1 BBa_B0034 range2489352 1 1 12 annotation2489354 1 scar range2489354 1 13 18 BBa_K1973003 1 BBa_K1973003 nahR-Psal-lapG 2016-10-10T11:00:00Z 2016-10-11T05:24:24Z Molecular cloning. We use the parts BBa_K1973033 and BBa_K1973000 to generate this part <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>lapG</i> (see BBa_K1973000) is expressed from the P<i>sal<i> promoter in the presence of this molecule. false false _2440_ 30017 30017 9 false We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493029 1 BBa_K1973033 component2493033 1 BBa_K1973000 annotation2493033 1 BBa_K1973000 range2493033 1 1048 1710 annotation2493029 1 BBa_K1973033 range2493029 1 1 1039 BBa_K1973033 1 BBa_K1973033 nahR-Psal (inducible promoter) 2016-10-08T11:00:00Z 2016-10-09T11:17:26Z It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed. nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. false false _2440_ 30003 30003 9 false We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece. false Laura Claret Fern??ndez annotation2489302 1 nahR range2489302 1 1 900 annotation2489301 1 Psal range2489301 1 901 1039 BBa_K1973000_sequence 1 aaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa BBa_K1973033_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc BBa_K1973003_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagagaaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z