BBa_K1973000
1
BBa_K1973000
lapG
2016-10-08T11:00:00Z
2016-10-12T05:16:10Z
Obtained by PCR from the genome of Pseudomonas putida KT2442.
Codifies the LapG protein from Pseudomonas putida KT2442, a periplasmic protease that participates in the biofilm dispersion process by cleaving adhesion proteins such as LapA.
false
false
_2440_
30017
30003
9
false
We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts
false
Laura Claret Fern??ndez
annotation2489353
1
lapG
range2489353
1
19
663
annotation2489352
1
BBa_B0034
range2489352
1
1
12
annotation2489354
1
scar
range2489354
1
13
18
BBa_K1973003
1
BBa_K1973003
nahR-Psal-lapG
2016-10-10T11:00:00Z
2016-10-11T05:24:24Z
Molecular cloning. We use the parts BBa_K1973033 and BBa_K1973000 to generate this part
<i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>lapG</i> (see BBa_K1973000) is expressed from the P<i>sal<i> promoter in the presence of this molecule.
false
false
_2440_
30017
30017
9
false
We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
false
Alvaro Escobar Doncel
component2493029
1
BBa_K1973033
component2493033
1
BBa_K1973000
annotation2493033
1
BBa_K1973000
range2493033
1
1048
1710
annotation2493029
1
BBa_K1973033
range2493029
1
1
1039
BBa_K1973033
1
BBa_K1973033
nahR-Psal (inducible promoter)
2016-10-08T11:00:00Z
2016-10-09T11:17:26Z
It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.
false
false
_2440_
30003
30003
9
false
We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.
false
Laura Claret Fern??ndez
annotation2489302
1
nahR
range2489302
1
1
900
annotation2489301
1
Psal
range2489301
1
901
1039
BBa_K1973000_sequence
1
aaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa
BBa_K1973033_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
BBa_K1973003_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagagaaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z