BBa_K1973005
1
BBa_K1973005
nahR-Psal-nasF
2016-10-10T11:00:00Z
2016-10-11T05:51:56Z
Molecular cloning.
<i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>nasF</i> (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from <i>Klebsiella pneumoniae</i> that decreases the basal expression from the P<i>sal</i> promoter.
false
false
_2440_
0
30017
30017
9
It's complicated
false
We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
false
Alvaro Escobar Doncel
component2493059
1
BBa_K1973033
component2493061
1
BBa_K1973001
annotation2493059
1
BBa_K1973033
range2493059
1
1
1039
annotation2493061
1
BBa_K1973001
range2493061
1
1048
1151
BBa_K1973033
1
BBa_K1973033
nahR-Psal (inducible promoter)
2016-10-08T11:00:00Z
2016-10-09T11:17:26Z
It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.
false
false
_2440_
30003
30003
9
false
We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.
false
Laura Claret Fern??ndez
annotation2489302
1
nahR
range2489302
1
1
900
annotation2489301
1
Psal
range2489301
1
901
1039
BBa_K1973001
1
BBa_K1973001
nasF
2016-10-08T11:00:00Z
2016-10-09T11:39:45Z
It was obtained by PCR from a plasmid containing its sequence.
nasF is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The nasR protein disrupts the terminator structure and allows the expression again, providing an useful tool for tight ON/OFF expression systems.
false
false
_2440_
30003
30003
9
false
No special considerations.
false
Laura Claret Fern??ndez
annotation2489358
1
nasF transcription terminator
range2489358
1
1
104
BBa_K1973033_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
BBa_K1973001_sequence
1
gagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc
BBa_K1973005_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z