BBa_K1973007 1 BBa_K1973007 nahR-Psal-nasF-lapG 2016-10-10T11:00:00Z 2016-10-11T05:59:00Z Molecular cloning. <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>lapG</i> (see BBa_K1973000) is expressed from the P<i>sal</i> promoter in the presence of this molecule. <i>nasF</i> (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from <i>Klebsiella pneumoniae</i> that decreases the basal expression from the P<i>sal</i> promoter. false false _2440_ 0 30017 30017 9 It's complicated false We digested BBa_K1973000 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493067 1 BBa_K1973005 component2493071 1 BBa_K1973000 annotation2493071 1 BBa_K1973000 range2493071 1 1160 1822 annotation2493067 1 BBa_K1973005 range2493067 1 1 1151 BBa_K1973005 1 BBa_K1973005 nahR-Psal-nasF 2016-10-10T11:00:00Z 2016-10-11T05:51:56Z Molecular cloning. <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>nasF</i> (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from <i>Klebsiella pneumoniae</i> that decreases the basal expression from the P<i>sal</i> promoter. false false _2440_ 0 30017 30017 9 It's complicated false We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493059 1 BBa_K1973033 component2493061 1 BBa_K1973001 annotation2493059 1 BBa_K1973033 range2493059 1 1 1039 annotation2493061 1 BBa_K1973001 range2493061 1 1048 1151 BBa_K1973000 1 BBa_K1973000 lapG 2016-10-08T11:00:00Z 2016-10-12T05:16:10Z Obtained by PCR from the genome of Pseudomonas putida KT2442. Codifies the LapG protein from Pseudomonas putida KT2442, a periplasmic protease that participates in the biofilm dispersion process by cleaving adhesion proteins such as LapA. false false _2440_ 30017 30003 9 false We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts false Laura Claret Fern??ndez annotation2489353 1 lapG range2489353 1 19 663 annotation2489352 1 BBa_B0034 range2489352 1 1 12 annotation2489354 1 scar range2489354 1 13 18 BBa_K1973033 1 BBa_K1973033 nahR-Psal (inducible promoter) 2016-10-08T11:00:00Z 2016-10-09T11:17:26Z It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed. nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. false false _2440_ 30003 30003 9 false We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece. false Laura Claret Fern??ndez annotation2489302 1 nahR range2489302 1 1 900 annotation2489301 1 Psal range2489301 1 901 1039 BBa_K1973001 1 BBa_K1973001 nasF 2016-10-08T11:00:00Z 2016-10-09T11:39:45Z It was obtained by PCR from a plasmid containing its sequence. nasF is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The nasR protein disrupts the terminator structure and allows the expression again, providing an useful tool for tight ON/OFF expression systems. false false _2440_ 30003 30003 9 false No special considerations. false Laura Claret Fern??ndez annotation2489358 1 nasF transcription terminator range2489358 1 1 104 BBa_K1973007_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgctactagagaaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa BBa_K1973000_sequence 1 aaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa BBa_K1973033_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc BBa_K1973001_sequence 1 gagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc BBa_K1973005_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z