LOCUS BBa_K1973009 6218 bp DNA linear 14-Nov-2024 DEFINITION miniTn7BB-Gm-nahR-Psal-nasF-lapG ACCESSION BBa_K1973009 VERSION BBa_K1973009.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix stem_loop 22..93 /label=E. coli his operon terminator primer_bind 157..176 /label=VR (BBa_G00101) primer binding site misc_feature 182..347 /label=Tn7-L end misc_feature 348..360 /label=SfiI restriction site CDS 966..1826 /label=Ampicillin resistance (bla) misc_feature 1965..2644 /label=ColEI (pUC18) Replication origin misc_feature 3002..3014 /label=SfiI restriction site misc_feature 3014..3213 /label=Tn7-R end misc_feature 3329..3376 /label=Flipase recombination sequence (FRT) CDS 3393..3926 /label=Gentamycin resistance misc_feature 4196..4243 /label=Flipase recombination sequence (FRT) misc_feature 4367..4387 /label=Prefix misc_feature 3224..3318 /label=double terminator (BBa_B0014) misc_feature 3381..3386 /label=SphI restriction site misc_feature 3387..3392 /label=NcoI restrictino site misc_feature 4184..4189 /label=NcoI restriction site misc_feature 4190..4195 /label=SphI restriction site primer_bind 4249..4268 /label=VF2 (G00100) primer binding site stem_loop 4326..4359 /label=Terminator misc_feature 1..4388 /label=BBa_K510000 promoter 5297..5435 /label=Psal CDS 4397..5296 /label=nahR promoter 4397..5435 /label=BBa_K1973033 misc_feature 5444..5547 /label=nasF transcription terminator terminator 5444..5547 /label=BBa_K1973001 misc_feature 4397..5547 /label=BBa_K1973005 RBS 5556..5567 /label=BBa_B0034 CDS 5574..6218 /label=lapG misc_feature 5568..5573 /label=scar CDS 5556..6218 /label=BBa_K1973000 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg 2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 3001 tggccgccta ggcctgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 3061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 3121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 3181 gcaaattgcc cgtcgtatta aagaggggcg tggggttcgc ttgtcacact ggctcacctt 3241 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat 3301 ccggcttttt tattatttgc tagatcttga agtacctatt ccgaagttcc tattctctaa 3361 aaagtatagg aacttcagag gcatgcccat ggttaggtgg cggtacttgg gtcgatatca 3421 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca 3481 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg 3541 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca 3601 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg 3661 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag 3721 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca 3781 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat 3841 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc 3901 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 3961 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata 4021 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga 4081 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt 4141 caattcgatc cattgctgtt gacaaaggga atcaggggat cttccatggg catgcgaagt 4201 acctattccg aagttcctat tctctaaaaa gtataggaac ttcagagctg ccacctgacg 4261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 4321 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 4381 ttctagagta ctagagtcaa tccgtaaaca ggtcaaacat cagttgccgc aaccaaatat 4441 tggctaggtc cttgtggtac ttcgcatgcc agaacatgtt gatggctatt tcaggcaaga 4501 cgactgggtg cggcaaggcg cttaggccga agggctccac gcagcagtcg gctaaacgta 4561 tcggcacagt ggcgagcaga tcggtgcgct ggaggatgtg gccaacggcg gcgaagtgcg 4621 gcacttccag acggatgtcg cgccggatgc cgacccgtgt catgtacgtg tccacctcgc 4681 cgtggccggt gccagcggcg atgacacgca cgtggccgta ggaacagaag cgctccagag 4741 tcaggggttc gcgggtgact ggatggtcct tgcgacatag gcacacgtag tgattctgga 4801 gcagccggcg ctgaaagaag ccagtttgca gattgggaag caggcccacg gccaagtcca 4861 cggttccgtt ctgcaaggcc tgcatcaggc tcatcgaact gtcgcgcacc gtactgatca 4921 cgcaattggg ggcctggtga gccagcacat ccatcagccg cggcatgaag tagatctcgc 4981 caatgtcggt catggccagg gtgaaggtac gctcgctggt cagcggatcg aagctttcat 5041 ggtgctgtag ggcgttgcgc agtgcgtgca tggccgaagt gacgggctcg gccagatgcg 5101 cggcataggg tgtgggttcc attccctgat gtgtgcgcac gaagagtggg tcctgtagcg 5161 aggtgcgcag gcgtttcagc gcattgctca cggcaggctg ggtcaggccc aggttctccg 5221 cagtgataga gacgcgtctg tcgaccagca actggttgaa caccaccagc aggtttaaat 5281 ccaggtcacg cagttccatg gggcctcgct tgggttattg ctggtgcccg gccgggcgca 5341 atattcatgt tgatgattta ttatatatcg agtggtgtat ttatcaatat tgtttgctcc 5401 gttatcgtta ttaacaagtc atcaataaag ccatctacta gaggagtgaa taaaaggttt 5461 tgggcagcgc gccaatggcg gcgcgtatgt ccagggataa aggcgtccag cggtgcgtaa 5521 gcaccgccgg gcgctttttt tttgcgctac tagagaaaga ggagaaatac tagatgctgc 5581 tgggcagtct tctgctgggc ggcttgcacg cggattggga tttctcccag atcagccgca 5641 agtcgcaggc tctgtacggc ccactaggtg ccgggcaggg tcgcatcgat gcctggcaga 5701 gcctgatggc cacgcagaag cagggtaccg agcttgaaag gttgcaagtg gtcaaccgct 5761 tcttcaacca gcaattgcgg tatgtcgagg atatcgatct gtggcatgag gtcgactact 5821 gggccacacc tgtgcaggca ctgatcaaag gtgcagggga ctgcgaggat tacgccatcg 5881 ccaaatattt cagtctacgg cgcatgggaa tccccagcga aaaactgcgc atcacctacg 5941 tcaaggcgct gcggcagaac cgggcacaca tggtcctgac ctattattca agcccacagg 6001 cccagccttt ggtgctggac agtctgatgg atgccataaa gccggcaagc cagcgtaccg 6061 acctgctgcc ggtgtacgcc ttcaatggcg aggggttgtg gctgacgggc gcggcaggga 6121 acaagaaggt tggtgatacc aagcggctgt cacgctggca ggatttgttg aagaaaatgc 6181 aggccgaagg gttcccggcc gagccggttt actaataa //