BBa_K1973009
type: ComponentDefinition
persistent identity: BBa K1973009
display id: BBa_K1973009
version: 1
was generated by: iGEM to SBOL conversion
description: miniTn7BB-Gm-nahR-Psal-nasF-lapG
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T09:28:12Z
mutable provenance: Basic parts were cloned into the miniTn7BB-Gm Backbone
owned by: james
owned by: myers
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).
discontinued: false
dominant: false
group u list: _2440_
m user id: 29968
owner id: 29968
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Designs are described in basic parts pages
star: false
creator: Gabriel Ruiz Romero
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_K1973000
component: BBa_K1973005
sequence annotation: BBa_K510000
sequence annotation: BBa_K1973005
sequence annotation: BBa_K1973000
sequence: https://synbiohub.org/public/igem/BBa_K1973009_sequence/1

BBa_K510000
type: Component
persistent identity: component2498537
display id: component2498537
version: 1
top level: BBa_K1973009
definition: BBa_K510000
access: public

BBa_K1973000
type: Component
persistent identity: component2498547
display id: component2498547
version: 1
top level: BBa_K1973009
definition: BBa_K1973000
access: public

BBa_K1973005
type: Component
persistent identity: component2498543
display id: component2498543
version: 1
top level: BBa_K1973009
definition: BBa_K1973005
access: public

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2498537
display id: annotation2498537
version: 1
top level: BBa_K1973009
location: https://synbiohub.org/public/igem/BBa_K1973009/annotation2498537/range2498537/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K1973009/annotation2498537/range2498537/1
type: Range
persistent identity: range2498537
display id: range2498537
version: 1
top level: BBa_K1973009
direction: direction/reverse
start: 1
end: 4388
orientation: inline

BBa_K1973005
type: SequenceAnnotation
persistent identity: annotation2498543
display id: annotation2498543
version: 1
top level: BBa_K1973009
location: https://synbiohub.org/public/igem/BBa_K1973009/annotation2498543/range2498543/1
component: BBa_K1973005

https://synbiohub.org/public/igem/BBa_K1973009/annotation2498543/range2498543/1
type: Range
persistent identity: range2498543
display id: range2498543
version: 1
top level: BBa_K1973009
direction: direction/reverse
start: 4397
end: 5547
orientation: inline

BBa_K1973000
type: SequenceAnnotation
persistent identity: annotation2498547
display id: annotation2498547
version: 1
top level: BBa_K1973009
location: https://synbiohub.org/public/igem/BBa_K1973009/annotation2498547/range2498547/1
component: BBa_K1973000

https://synbiohub.org/public/igem/BBa_K1973009/annotation2498547/range2498547/1
type: Range
persistent identity: range2498547
display id: range2498547
version: 1
top level: BBa_K1973009
direction: direction/reverse
start: 5556
end: 6218
orientation: inline

BBa_K1973000
type: ComponentDefinition
persistent identity: BBa K1973000
display id: BBa_K1973000
version: 1
was generated by: iGEM to SBOL conversion
description: lapG
created: 2016-10-08T11:00:00Z
modified: 2016-10-12T05:16:10Z
mutable provenance: Obtained by PCR from the genome of Pseudomonas putida KT2442.
owned by: james
owned by: myers
mutable description: Codifies the LapG protein from Pseudomonas putida KT2442, a periplasmic protease that participates in the biofilm dispersion process by cleaving adhesion proteins such as LapA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2440_
m user id: 30017
owner id: 30003
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts
star: false
creator: Laura Claret Fern??ndez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_B0034
sequence annotation: scar
sequence annotation: lapG
sequence: https://synbiohub.org/public/igem/BBa_K1973000_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2489352
display id: annotation2489352
version: 1
top level: BBa_K1973000
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489352/range2489352/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489352/range2489352/1
type: Range
persistent identity: range2489352
display id: range2489352
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 1
end: 12
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2489354
display id: annotation2489354
version: 1
top level: BBa_K1973000
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489354/range2489354/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489354/range2489354/1
type: Range
persistent identity: range2489354
display id: range2489354
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 13
end: 18
orientation: inline

lapG
type: SequenceAnnotation
persistent identity: annotation2489353
display id: annotation2489353
version: 1
top level: BBa_K1973000
location: https://synbiohub.org/public/igem/BBa_K1973000/annotation2489353/range2489353/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1973000/annotation2489353/range2489353/1
type: Range
persistent identity: range2489353
display id: range2489353
version: 1
top level: BBa_K1973000
direction: direction/forward
start: 19
end: 663
orientation: inline

BBa_K1973005
type: ComponentDefinition
persistent identity: BBa K1973005
display id: BBa_K1973005
version: 1
was generated by: iGEM to SBOL conversion
description: nahR-Psal-nasF
created: 2016-10-10T11:00:00Z
modified: 2016-10-11T05:51:56Z
mutable provenance: Molecular cloning.
owned by: james
owned by: myers
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.
discontinued: false
dominant: false
group u list: _2440_
m user id: 0
m user id: 30017
owner id: 30017
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
star: false
creator: Alvaro Escobar Doncel
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1973001
component: BBa_K1973033
sequence annotation: BBa_K1973033
sequence annotation: BBa_K1973001
sequence: https://synbiohub.org/public/igem/BBa_K1973005_sequence/1

BBa_K1973001
type: Component
persistent identity: component2493061
display id: component2493061
version: 1
top level: BBa_K1973005
definition: BBa_K1973001
access: public

BBa_K1973033
type: Component
persistent identity: component2493059
display id: component2493059
version: 1
top level: BBa_K1973005
definition: BBa_K1973033
access: public

BBa_K1973033
type: SequenceAnnotation
persistent identity: annotation2493059
display id: annotation2493059
version: 1
top level: BBa_K1973005
location: https://synbiohub.org/public/igem/BBa_K1973005/annotation2493059/range2493059/1
component: BBa_K1973033

https://synbiohub.org/public/igem/BBa_K1973005/annotation2493059/range2493059/1
type: Range
persistent identity: range2493059
display id: range2493059
version: 1
top level: BBa_K1973005
direction: direction/reverse
start: 1
end: 1039
orientation: inline

BBa_K1973001
type: SequenceAnnotation
persistent identity: annotation2493061
display id: annotation2493061
version: 1
top level: BBa_K1973005
location: https://synbiohub.org/public/igem/BBa_K1973005/annotation2493061/range2493061/1
component: BBa_K1973001

https://synbiohub.org/public/igem/BBa_K1973005/annotation2493061/range2493061/1
type: Range
persistent identity: range2493061
display id: range2493061
version: 1
top level: BBa_K1973005
direction: direction/reverse
start: 1048
end: 1151
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restrictino site
sequence annotation: Gentamycin resistance
sequence annotation: NcoI restriction site
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
type: Range
persistent identity: range2137411
display id: range2137411
version: 1
top level: BBa_K510000
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
type: Range
persistent identity: range2137412
display id: range2137412
version: 1
top level: BBa_K510000
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
type: Range
persistent identity: range2137413
display id: range2137413
version: 1
top level: BBa_K510000
direction: direction/forward
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
type: Range
persistent identity: range2137414
display id: range2137414
version: 1
top level: BBa_K510000
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
top level: BBa_K510000
direction: direction/forward
start: 348
end: 360
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
type: Range
persistent identity: range2137416
display id: range2137416
version: 1
top level: BBa_K510000
direction: direction/forward
start: 966
end: 1826
orientation: inline

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
top level: BBa_K510000
direction: direction/forward
start: 1965
end: 2644
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3002
end: 3014
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3014
end: 3213
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3224
end: 3318
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3329
end: 3376
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3381
end: 3386
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3387
end: 3392
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
top level: BBa_K510000
direction: direction/reverse
start: 3393
end: 3926
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4184
end: 4189
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
type: Range
persistent identity: range2137440
display id: range2137440
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4196
end: 4243
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4249
end: 4268
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4326
end: 4359
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4367
end: 4387
orientation: inline

BBa_K1973033
type: ComponentDefinition
persistent identity: BBa K1973033
display id: BBa_K1973033
version: 1
was generated by: iGEM to SBOL conversion
description: nahR-Psal (inducible promoter)
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T11:17:26Z
mutable provenance: It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.
owned by: james
owned by: myers
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.
discontinued: false
dominant: false
group u list: _2440_
m user id: 30003
owner id: 30003
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.
star: false
creator: Laura Claret Fern??ndez
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: nahR
sequence annotation: Psal
sequence: https://synbiohub.org/public/igem/BBa_K1973033_sequence/1

nahR
type: SequenceAnnotation
persistent identity: annotation2489302
display id: annotation2489302
version: 1
top level: BBa_K1973033
location: https://synbiohub.org/public/igem/BBa_K1973033/annotation2489302/range2489302/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1973033/annotation2489302/range2489302/1
type: Range
persistent identity: range2489302
display id: range2489302
version: 1
top level: BBa_K1973033
direction: direction/reverse
start: 1
end: 900
orientation: inline

Psal
type: SequenceAnnotation
persistent identity: annotation2489301
display id: annotation2489301
version: 1
top level: BBa_K1973033
location: https://synbiohub.org/public/igem/BBa_K1973033/annotation2489301/range2489301/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1973033/annotation2489301/range2489301/1
type: Range
persistent identity: range2489301
display id: range2489301
version: 1
top level: BBa_K1973033
direction: direction/forward
start: 901
end: 1039
orientation: inline

BBa_K1973001
type: ComponentDefinition
persistent identity: BBa K1973001
display id: BBa_K1973001
version: 1
was generated by: iGEM to SBOL conversion
description: nasF
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T11:39:45Z
mutable provenance: It was obtained by PCR from a plasmid containing its sequence.
owned by: james
owned by: myers
mutable description: nasF is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The nasR protein disrupts the terminator structure and allows the expression again, providing an useful tool for tight ON/OFF expression systems.
discontinued: false
dominant: false
group u list: _2440_
m user id: 30003
owner id: 30003
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special considerations.
star: false
creator: Laura Claret Fern??ndez
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: nasF transcription terminator
sequence: https://synbiohub.org/public/igem/BBa_K1973001_sequence/1

nasF transcription terminator
type: SequenceAnnotation
persistent identity: annotation2489358
display id: annotation2489358
version: 1
top level: BBa_K1973001
location: https://synbiohub.org/public/igem/BBa_K1973001/annotation2489358/range2489358/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1973001/annotation2489358/range2489358/1
type: Range
persistent identity: range2489358
display id: range2489358
version: 1
top level: BBa_K1973001
direction: direction/forward
start: 1
end: 104
orientation: inline

https://synbiohub.org/public/igem/BBa_K1973009_sequence/1
type: Sequence
persistent identity: BBa K1973009 sequence
display id: BBa_K1973009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973000_sequence/1
type: Sequence
persistent identity: BBa K1973000 sequence
display id: BBa_K1973000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgctgctgggcagtcttctgctgggcggcttgcacgcggattgggatttctcccagatcagccgcaagtcgcaggctctgtacggcccactaggtgccgggcagggtcgcatcgatgcctggcagagcctgatggccacgcagaagcagggtaccgagcttgaaaggttgcaagtggtcaaccgcttcttcaaccagcaattgcggtatgtcgaggatatcgatctgtggcatgaggtcgactactgggccacacctgtgcaggcactgatcaaaggtgcaggggactgcgaggattacgccatcgccaaatatttcagtctacggcgcatgggaatccccagcgaaaaactgcgcatcacctacgtcaaggcgctgcggcagaaccgggcacacatggtcctgacctattattcaagcccacaggcccagcctttggtgctggacagtctgatggatgccataaagccggcaagccagcgtaccgacctgctgccggtgtacgccttcaatggcgaggggttgtggctgacgggcgcggcagggaacaagaaggttggtgataccaagcggctgtcacgctggcaggatttgttgaagaaaatgcaggccgaagggttcccggccgagccggtttactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973005_sequence/1
type: Sequence
persistent identity: BBa K1973005 sequence
display id: BBa_K1973005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510000_sequence/1
type: Sequence
persistent identity: BBa K510000 sequence
display id: BBa_K510000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973033_sequence/1
type: Sequence
persistent identity: BBa K1973033 sequence
display id: BBa_K1973033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1973001_sequence/1
type: Sequence
persistent identity: BBa K1973001 sequence
display id: BBa_K1973001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z