BBa_K1973011
1
BBa_K1973011
nahR-Psal-pleD*
2016-10-11T11:00:00Z
2016-10-12T03:39:50Z
Molecular cloning.
<i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>pleD*</i> (see BBa_K1973016) is expressed from the P<i>sal/i>promoter in the presence of this molecule.
false
false
_2440_
30017
30017
9
false
We digested BBa_K1973016 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
false
Alvaro Escobar Doncel
component2493611
1
BBa_K1973016
component2493607
1
BBa_K1973033
annotation2493611
1
BBa_K1973016
range2493611
1
1048
2432
annotation2493607
1
BBa_K1973033
range2493607
1
1
1039
BBa_K1973033
1
BBa_K1973033
nahR-Psal (inducible promoter)
2016-10-08T11:00:00Z
2016-10-09T11:17:26Z
It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.
false
false
_2440_
30003
30003
9
false
We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.
false
Laura Claret Fern??ndez
annotation2489302
1
nahR
range2489302
1
1
900
annotation2489301
1
Psal
range2489301
1
901
1039
BBa_K1973016
1
BBa_K1973016
pleD*
2016-10-08T11:00:00Z
2016-10-13T10:43:08Z
It was obtained form a plasmid that contained its sequence.
pleD* BBa_K1973016 Codifies the PleD* protein from Caulobacter crescentus, which is a diguanilate cyclase that induces the biofilm formation by increasing c-di-GMP (cyclic diguanilate monophosphate) levels. c-diGMP regulates both the formation and dispersion of the biofilm, depending on the quantity of it that is present inside the cell. High levels of c-diGMP will increase the biofilm formation, and low levels of it will induce its dispersion. To manipulate this, we will make use of a phosphodiesterase, that will destroy the c-diGMP, and a diguanilate cyclase, that will produce it, YhjH and PleD* respectively.
Contains a constitutive mutation in the original sequence (see BBa_K2012002) that improves the enzymatic activity by knocking out the regulation system.
false
false
_2440_
29968
30003
9
true
We have included the sequence of RBS BBa_B0034 by means of the PCR primers and amplification, as well as the scar that should have been in the clonning of this part.
false
Laura Claret Fern??ndez
annotation2489372
1
BBa_B0034
range2489372
1
1
12
annotation2489373
1
scar
range2489373
1
13
18
annotation2489374
1
pleD*
range2489374
1
19
1385
BBa_K1973011_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagagaaagaggagaaatactagatgagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcatctgcccgacgatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctaaccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgctgatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcgccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcacgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctgcactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgtacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgcctaataa
BBa_K1973033_sequence
1
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
BBa_K1973016_sequence
1
aaagaggagaaatactagatgagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcatctgcccgacgatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctaaccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgctgatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcgccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcacgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctgcactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgtacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z