BBa_K1973005 1 BBa_K1973005 nahR-Psal-nasF 2016-10-10T11:00:00Z 2016-10-11T05:51:56Z Molecular cloning. <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>nasF</i> (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from <i>Klebsiella pneumoniae</i> that decreases the basal expression from the P<i>sal</i> promoter. false false _2440_ 0 30017 30017 9 It's complicated false We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493059 1 BBa_K1973033 component2493061 1 BBa_K1973001 annotation2493061 1 BBa_K1973001 range2493061 1 1048 1151 annotation2493059 1 BBa_K1973033 range2493059 1 1 1039 BBa_K1973001 1 BBa_K1973001 nasF 2016-10-08T11:00:00Z 2016-10-09T11:39:45Z It was obtained by PCR from a plasmid containing its sequence. nasF is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The nasR protein disrupts the terminator structure and allows the expression again, providing an useful tool for tight ON/OFF expression systems. false false _2440_ 30003 30003 9 false No special considerations. false Laura Claret Fern??ndez annotation2489358 1 nasF transcription terminator range2489358 1 1 104 BBa_K1973017 1 BBa_K1973017 nahR-Psal-nasF-xylS2 2016-10-11T11:00:00Z 2016-10-12T03:58:52Z Molecular cloning. <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>nasF</i> (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from <i>Klebsiella pneumoniae</i> that decreases the basal expression from the P<i>sal</i> promoter. <i>xylS2</i> (BBa_K1973002) codifies the modified XylS2 protein from <i>Pseudomonas putida</i> MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system. false false _2440_ 30017 30017 9 false We digested BBa_K1973002 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493673 1 BBa_K1973002 component2493669 1 BBa_K1973005 annotation2493673 1 BBa_K1973002 range2493673 1 1160 2146 annotation2493669 1 BBa_K1973005 range2493669 1 1 1151 BBa_K1973002 1 BBa_K1973002 xylS2 2016-10-08T11:00:00Z 2016-10-09T11:44:17Z It was obtained by PCR from a plasmid containing its sequence. Codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. The modification allows the response to salicylate. See BBa_K1031911 (original version of this part, which is regulated by benzoate and derivatives of this). false false _2440_ 30003 30003 9 false We have inlcuded the sequence of RBS BBa_B0034 by means of the PCR primers in the amplification, as well as the scar that should have been when clonning this part. false Laura Claret Fern??ndez annotation2489360 1 scar range2489360 1 13 18 annotation2489359 1 BBa_B0034 range2489359 1 1 12 annotation2489361 1 xylS2 range2489361 1 19 987 BBa_K1973033 1 BBa_K1973033 nahR-Psal (inducible promoter) 2016-10-08T11:00:00Z 2016-10-09T11:17:26Z It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed. nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. false false _2440_ 30003 30003 9 false We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece. false Laura Claret Fern??ndez annotation2489301 1 Psal range2489301 1 901 1039 annotation2489302 1 nahR range2489302 1 1 900 BBa_K1973033_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc BBa_K1973002_sequence 1 aaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcacgcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttaataa BBa_K1973001_sequence 1 gagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc BBa_K1973005_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgc BBa_K1973017_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagaggagtgaataaaaggttttgggcagcgcgccaatggcggcgcgtatgtccagggataaaggcgtccagcggtgcgtaagcaccgccgggcgctttttttttgcgctactagagaaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcacgcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z