BBa_K1973002 1 BBa_K1973002 xylS2 2016-10-08T11:00:00Z 2016-10-09T11:44:17Z It was obtained by PCR from a plasmid containing its sequence. Codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. The modification allows the response to salicylate. See BBa_K1031911 (original version of this part, which is regulated by benzoate and derivatives of this). false false _2440_ 30003 30003 9 false We have inlcuded the sequence of RBS BBa_B0034 by means of the PCR primers in the amplification, as well as the scar that should have been when clonning this part. false Laura Claret Fern??ndez annotation2489360 1 scar range2489360 1 13 18 annotation2489359 1 BBa_B0034 range2489359 1 1 12 annotation2489361 1 xylS2 range2489361 1 19 987 BBa_K1973020 1 BBa_K1973020 nahR-Psal-xylS2 2016-10-11T11:00:00Z 2016-10-12T03:58:27Z Molecular cloning. <i>nahR</i> (<i>Pseudomonas putida</i>, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the P<i>sal</i> promoter (see BBa_J61051) in the presence of salicylate. <i>xylS2</i> (BBa_K1973002) is expressed from the P<i>sal</i> promoter in the presence of this molecule. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system. false false _2440_ 30017 30017 9 false We digested BBa_K1973002 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them. false Alvaro Escobar Doncel component2493659 1 BBa_K1973033 component2493663 1 BBa_K1973002 annotation2493659 1 BBa_K1973033 range2493659 1 1 1039 annotation2493663 1 BBa_K1973002 range2493663 1 1048 2034 BBa_K1973033 1 BBa_K1973033 nahR-Psal (inducible promoter) 2016-10-08T11:00:00Z 2016-10-09T11:17:26Z It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed. nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. false false _2440_ 30003 30003 9 false We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece. false Laura Claret Fern??ndez annotation2489302 1 nahR range2489302 1 1 900 annotation2489301 1 Psal range2489301 1 901 1039 BBa_K1973020_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagagaaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcacgcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttaataa BBa_K1973033_sequence 1 tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc BBa_K1973002_sequence 1 aaagaggagaaatactagatggatttttgcttattgaacgagaaaagtcagatcttcgtccacgccgagccctatgcagtctccgattatgttaaccagtatgtcggtacgcactctattcgcctgcccaagggcgggcgcccggcacgcaggctgcaccacagaatcttcggatgcctcgacctgtgtcgaatcagctacggcggtagcgtgagggtaatctcgcctggattagagacctgttatcatctgcaaataatactcaaaggccattgcctgtggcgtggccatggccaggagcactattttgcgccgggcgaactattgctgctcaatccggatgaccaagccgacctgacctattcagaagattgcgagaaatttatcgttaaattgccctcagtggtccttgatcgggcatgcagtgacaacaattggcacaagccgagggagggtatccgtttcgccgcgcgacacaatctccagcaactcgatggctttatcaatctactcgggttagtttgtgacgaagcggaacatacaaagtcgatgcctcgggtccaagagcactatgcggggatcatcgcttccaagctgctcgaaatgctgggcagcaatgtcagccgtgaaattttcagcaaaggtaacccgtctttcgagcgagtcgttcaattcattgaggagaatctcaaacggaatatcagccttgagcggttagcggagctggcgatgatgagtccacgctcgctctacaatttgttcgagaagcatgccggcaccacgccgaagaactacatccgcaaccgcaagctcgaaagcatccgcgcctgcttgaacgatcccagtgccaatgtgcgtagtataactgagatagccctagactacggcttcttacatttgggacgcttcgctgaaaactataggagcgcgttcggcgagttgccttccgacaccctgcgtcaatgcaaaaaggaagtggcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z