##gff-version 3
##sequence-region BBa_K1973023 1 6828
BBa_K1973023	.	CDS	5444	6828	.	+	0	ID=BBa_K1973016;Name=BBa_K1973016
BBa_K1973023	.	ribosome_entry_site	5444	5455	.	+	0	ID=annotation2489372;Name=BBa_B0034;Parent=BBa_K1973016
BBa_K1973023	.	null	5456	5461	.	+	0	ID=annotation2489373;Name=scar;Parent=BBa_K1973016
BBa_K1973023	.	CDS	5462	6828	.	+	0	ID=annotation2489374;Name=pleD*;Parent=BBa_K1973016
BBa_K1973023	.	promoter	4397	5435	.	+	0	ID=BBa_K1973033;Name=BBa_K1973033
BBa_K1973023	.	promoter	5297	5435	.	+	0	ID=annotation2489301;Name=Psal;Parent=BBa_K1973033
BBa_K1973023	.	CDS	4397	5296	.	+	0	ID=annotation2489302;Name=nahR;Parent=BBa_K1973033
BBa_K1973023	.	plasmid	1	4388	.	+	0	ID=BBa_K510000;Name=BBa_K510000
BBa_K1973023	.	sequence_feature	1965	2644	.	+	0	ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000
BBa_K1973023	.	engineered_region	4367	4387	.	+	0	ID=annotation2137423;Name=Prefix;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	348	360	.	+	0	ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	3381	3386	.	+	0	ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	3002	3014	.	+	0	ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	182	347	.	+	0	ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	4190	4195	.	+	0	ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	4196	4243	.	+	0	ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	4184	4189	.	+	0	ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000
BBa_K1973023	.	CDS	966	1826	.	+	0	ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	3329	3376	.	+	0	ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	3387	3392	.	+	0	ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000
BBa_K1973023	.	stem_loop	4326	4359	.	+	0	ID=annotation2137449;Name=Terminator;Parent=BBa_K510000
BBa_K1973023	.	primer_binding_site	157	176	.	+	0	ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000
BBa_K1973023	.	sequence_feature	3014	3213	.	+	0	ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000
BBa_K1973023	.	engineered_region	2	21	.	+	0	ID=annotation2137411;Name=Suffix;Parent=BBa_K510000
BBa_K1973023	.	engineered_region	3224	3318	.	+	0	ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000
BBa_K1973023	.	CDS	3393	3926	.	+	0	ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000
BBa_K1973023	.	primer_binding_site	4249	4268	.	+	0	ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000
BBa_K1973023	.	stem_loop	22	93	.	+	0	ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000
>BBa_K1973023
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt
gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac
gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc
cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg
tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct
atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc
cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat
attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt
ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg
gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc
tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt
ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca
tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac
gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt
agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta
ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac
caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct
gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga
gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt
tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat
taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa
gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg
gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac
tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca
aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg
tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc
gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg
gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact
tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg
gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga
tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta
actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac
atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat
gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg
cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat
gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagtcaatccgtaaacaggtcaaacatcagt
tgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacga
ctgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatc
ggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtc
atgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagag
tcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaa
gccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaa
ctgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatct
cgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgtt
gcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtg
cgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggt
tctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcag
ttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgag
tggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatctactagagaaagagga
gaaatactagatgagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacg
gccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcatctgcccgacgatca
ttctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgcca
catcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttc
ctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctaaccgcttcaagctggtgatcgacgaact
gcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgg
gtgctgatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcg
agagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaactt
cgatggcctgcgcttcgccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcac
gatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaac
tgtccgcgcgcgtcaagacgcagatccagcgcaagcgctgcactgactatctgcgcaacaatctggatcactcgctgga
gctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgc
gcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtc
acgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgtacgtccgcgccattgatctgccttgccg
ctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccgg
atgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcgg
ccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcggg
tcggaacgcggtggtcggcaaggccgcctaataa