BBa_K1973026
1
BBa_K1973026
glpF (membrane transporter)
2016-10-12T11:00:00Z
2016-10-13T08:04:04Z
Obtained by PCR from the genome of Pseudomonas aeruginosa PAO1
Codifies the GlpF protein from Pseudomonas aeruginosa, which is a glycerol channel that increases the glycerol intake for bioremediation purposes.
false
false
_2440_
29968
29968
9
false
It was amplified by mutagenic PCR. Nucleotide 693 (starting at ATG) was changed (from a cytosine to a thymine). We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts.
false
Gabriel Ruiz Romero
annotation2497821
1
C --> T
range2497821
1
711
711
annotation2497812
1
glpF
range2497812
1
19
861
annotation2497808
1
B0034
range2497808
1
1
12
annotation2497811
1
Scar
range2497811
1
13
18
BBa_K1973026_sequence
1
aaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z