BBa_K1973026 1 BBa_K1973026 glpF (membrane transporter) 2016-10-12T11:00:00Z 2016-10-13T08:04:04Z Obtained by PCR from the genome of Pseudomonas aeruginosa PAO1 Codifies the GlpF protein from Pseudomonas aeruginosa, which is a glycerol channel that increases the glycerol intake for bioremediation purposes. false false _2440_ 29968 29968 9 false It was amplified by mutagenic PCR. Nucleotide 693 (starting at ATG) was changed (from a cytosine to a thymine). We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts. false Gabriel Ruiz Romero annotation2497821 1 C --> T range2497821 1 711 711 annotation2497811 1 Scar range2497811 1 13 18 annotation2497808 1 B0034 range2497808 1 1 12 annotation2497812 1 glpF range2497812 1 19 861 BBa_K1973026_sequence 1 aaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z