BBa_K1973013
1
BBa_K1973013
Pm (1)
2016-10-08T11:00:00Z
2016-10-09T11:51:21Z
It was obtained by PCR from a plamid that had its sequence.
Pm1 BBa_K1973013 Pm1 is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively.
false
false
_2440_
30003
30003
9
false
It did have to be site-directed mutagenized in position 65 in order to remove a PstI restriction site.
false
Laura Claret Fern??ndez
annotation2489362
1
Pm (1)
range2489362
1
1
388
annotation2489363
1
A -> T mutation
range2489363
1
65
65
BBa_K1973026
1
BBa_K1973026
glpF (membrane transporter)
2016-10-12T11:00:00Z
2016-10-13T08:04:04Z
Obtained by PCR from the genome of Pseudomonas aeruginosa PAO1
Codifies the GlpF protein from Pseudomonas aeruginosa, which is a glycerol channel that increases the glycerol intake for bioremediation purposes.
false
false
_2440_
29968
29968
9
false
It was amplified by mutagenic PCR. Nucleotide 693 (starting at ATG) was changed (from a cytosine to a thymine). We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts.
false
Gabriel Ruiz Romero
annotation2497808
1
B0034
range2497808
1
1
12
annotation2497821
1
C --> T
range2497821
1
711
711
annotation2497811
1
Scar
range2497811
1
13
18
annotation2497812
1
glpF
range2497812
1
19
861
BBa_K1973035
1
BBa_K1973035
Pm1-glpF
2016-10-13T11:00:00Z
2016-10-14T03:56:26Z
This composite part was created by cloning the parts BBa_K1973013 and BBa_K1973026 together.
Pm1 (BBa_K1973013) is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively. In this construct, Pm1 controls the expression of glpF (BBa_K1973026), a glycerol transporter.
false
false
_2440_
29967
29967
9
false
This composite part was created by cloning the parts BBa_K1973013 and BBa_K1973026 together.
false
Jos?? Mart??n G??mez
component2501839
1
BBa_K1973026
component2501834
1
BBa_K1973013
annotation2501839
1
BBa_K1973026
range2501839
1
397
1257
annotation2501834
1
BBa_K1973013
range2501834
1
1
388
BBa_K1973026_sequence
1
aaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa
BBa_K1973035_sequence
1
tgatagagataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgacaatgcacctggggctcgactatatagatagtctcgttgaagaagatgagaacgagggcatctaccgctgcaagcgcgagatgttcaccgaccctcggctgttcgatttagagatgaaacacatctttgagggcaactggatttatctcgcccacgagagccagattcccgagaagaacgactattacaccacgcagatgggccggcagccgatattcatcacacgcaacaaagatggtgagctgaatgccttcgtcaatgccttactagagaaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa
BBa_K1973013_sequence
1
tgatagagataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgacaatgcacctggggctcgactatatagatagtctcgttgaagaagatgagaacgagggcatctaccgctgcaagcgcgagatgttcaccgaccctcggctgttcgatttagagatgaaacacatctttgagggcaactggatttatctcgcccacgagagccagattcccgagaagaacgactattacaccacgcagatgggccggcagccgatattcatcacacgcaacaaagatggtgagctgaatgccttcgtcaatgcct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z