BBa_K1973013 1 BBa_K1973013 Pm (1) 2016-10-08T11:00:00Z 2016-10-09T11:51:21Z It was obtained by PCR from a plamid that had its sequence. Pm1 BBa_K1973013 Pm1 is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively. false false _2440_ 30003 30003 9 false It did have to be site-directed mutagenized in position 65 in order to remove a PstI restriction site. false Laura Claret Fern??ndez annotation2489362 1 Pm (1) range2489362 1 1 388 annotation2489363 1 A -> T mutation range2489363 1 65 65 BBa_K1973026 1 BBa_K1973026 glpF (membrane transporter) 2016-10-12T11:00:00Z 2016-10-13T08:04:04Z Obtained by PCR from the genome of Pseudomonas aeruginosa PAO1 Codifies the GlpF protein from Pseudomonas aeruginosa, which is a glycerol channel that increases the glycerol intake for bioremediation purposes. false false _2440_ 29968 29968 9 false It was amplified by mutagenic PCR. Nucleotide 693 (starting at ATG) was changed (from a cytosine to a thymine). We have inlcuded the sequence corresponding to RBS BBa_B0034 with the PCR primers used for its amplification, as well as the scar it should have been produced by the clonning of both parts. false Gabriel Ruiz Romero annotation2497808 1 B0034 range2497808 1 1 12 annotation2497821 1 C --> T range2497821 1 711 711 annotation2497811 1 Scar range2497811 1 13 18 annotation2497812 1 glpF range2497812 1 19 861 BBa_K1973035 1 BBa_K1973035 Pm1-glpF 2016-10-13T11:00:00Z 2016-10-14T03:56:26Z This composite part was created by cloning the parts BBa_K1973013 and BBa_K1973026 together. Pm1 (BBa_K1973013) is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively. In this construct, Pm1 controls the expression of glpF (BBa_K1973026), a glycerol transporter. false false _2440_ 29967 29967 9 false This composite part was created by cloning the parts BBa_K1973013 and BBa_K1973026 together. false Jos?? Mart??n G??mez component2501839 1 BBa_K1973026 component2501834 1 BBa_K1973013 annotation2501839 1 BBa_K1973026 range2501839 1 397 1257 annotation2501834 1 BBa_K1973013 range2501834 1 1 388 BBa_K1973026_sequence 1 aaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa BBa_K1973035_sequence 1 tgatagagataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgacaatgcacctggggctcgactatatagatagtctcgttgaagaagatgagaacgagggcatctaccgctgcaagcgcgagatgttcaccgaccctcggctgttcgatttagagatgaaacacatctttgagggcaactggatttatctcgcccacgagagccagattcccgagaagaacgactattacaccacgcagatgggccggcagccgatattcatcacacgcaacaaagatggtgagctgaatgccttcgtcaatgccttactagagaaagaggagaaatactagatgaccaccgccgccccgaccccgtccctgttcggccaatgcctggccgagttcctcggcaccgccctcctcatcttcttcggcaccggttgcgtcgcggccctgaaggtcgccggcgccagcttcggcctgtgggaaatcagcatcatctggggggtcggcgtgagcatggcgatctacctcagcgccggcgtctccggcgcccacctgaacccggcggtgagcatcgcgctgtggctgttcgccggcttcgaggggcgcaagctgccgttctacatcaccgcccaggtggccggtgcgttctgcgccgcagcgctggtctacaccctctacagcagcctgttcatcgagttcgaacaggcgcagaacatcgtccgcggcagccaggacagcctggccctggcctcggtgttctccacctatccgcacccggcgctgtcggtcggccaggcgttcctcgtcgaagtggtgatcacggccatcctcatggcggtgatcatggcccttaccgacgacggcaacggcctgccgcgcggtccgttggcgccgctgctgatcggcctgctgatcgccgtgatcggtagcgccatgggcccgctgaccggtttcgcgatgaacccggcgcgcgacttcggccccaagctgatgacctacctggccggctgggggcccatcgccttcaccggcggccgtgagattccctattttctggtgccgatcttcgccccgatcctcggtgcctgcctcggcgccggcggctatcgtgtactgatcgcgcgccatctgccgagcgccgcggcgcccgccgaagccgagccggagaaggttcgcgcttcctaataa BBa_K1973013_sequence 1 tgatagagataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgacaatgcacctggggctcgactatatagatagtctcgttgaagaagatgagaacgagggcatctaccgctgcaagcgcgagatgttcaccgaccctcggctgttcgatttagagatgaaacacatctttgagggcaactggatttatctcgcccacgagagccagattcccgagaagaacgactattacaccacgcagatgggccggcagccgatattcatcacacgcaacaaagatggtgagctgaatgccttcgtcaatgcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z