LOCUS       BBa_K1976000            1260 bp    DNA     linear       23-Dec-2024
DEFINITION  Integration plasmid
ACCESSION   BBa_K1976000
VERSION     BBa_K1976000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..34
                     /label=Bidirectional Terminator
     misc_feature    35..266
                     /label=Lambda-attP site
     misc_feature    267..300
                     /label=Bidirectional Terminator
     misc_feature    1..300
                     /label=BBa_K1976004
     promoter        309..343
                     /label=BBa_J23101
     RBS             352..364
                     /label=RBS-3\Weak
     RBS             358..361
                     /label=RBS
     misc_feature    352..364
                     /label=BBa_B0032
     RBS             352..364
                     /label=BBa_B0032
     CDS             371..1084
                     /label=GFP
     misc_feature    1085..1117
                     /label=LVA-Tag
     CDS             371..1123
                     /label=BBa_K1976005
     stem_loop       1143..1186
                     /label=stem_loop
     misc_feature    1132..1211
                     /label=BBa_B0010
     terminator      1132..1211
                     /label=BBa_B0010
     stem_loop       1227..1246
                     /label=T7 TE
     misc_feature    1253..1253
                     /label=stop
     polyA_site      1247..1260
                     /label=polya
     misc_feature    1220..1260
                     /label=BBa_B0012
     terminator      1220..1260
                     /label=BBa_B0012
     misc_feature    352..1260
                     /label=BBa_K1976006
ORIGIN
        1 aaaaaaaaac cccgcttcgg cggggttttt tttttacagg tcactaatac catctaagta
       61 gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttttt
      181 atactaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      241 tatcagtcaa aataaaatca ttatttaaaa aaaaaccccg cttcggcggg gttttttttt
      301 tactagagtt tacagctagc tcagtcctag gtattatgct agctactaga gtcacacagg
      361 aaagtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      421 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      481 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      541 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      601 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      661 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      721 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      781 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      841 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      901 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      961 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1021 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1081 caaagcagca aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc
     1141 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1201 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//