LOCUS       BBa_K1976006             909 bp    DNA     linear       09-Apr-2025
DEFINITION  GFP generator with LVA-tag
ACCESSION   BBa_K1976006
VERSION     BBa_K1976006.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..733
                     /label=GFP
     misc_feature    734..766
                     /label=LVA-Tag
     CDS             20..772
                     /label=BBa_K1976005
     stem_loop       792..835
                     /label=stem_loop
     misc_feature    781..860
                     /label=BBa_B0010
     terminator      781..860
                     /label=BBa_B0010
     stem_loop       876..895
                     /label=T7 TE
     misc_feature    902..902
                     /label=stop
     polyA_site      896..909
                     /label=polya
     misc_feature    869..909
                     /label=BBa_B0012
     terminator      869..909
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaagcagcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag
      781 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      841 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      901 gcgtttata
//