BBa_K1976033 1 BBa_K1976033 Inducible generator for colicin E2 immunity protein (+amber codon) 2016-10-12T11:00:00Z 2016-10-19T03:08:11Z Escherichia coli Inducible generator for immunity protein for Colicin E2 (+amber codon) false false _2443_ 25991 30994 9 false Synthesis feasible by IDT, no amber stop codon for termination false Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Sch&#228;fer, Inka Schr&#246;ter, Viktoria Schuster component2498082 1 BBa_K1976026 component2498077 1 BBa_K525998 component2498079 1 BBa_B0034 annotation2498079 1 BBa_B0034 range2498079 1 41 52 annotation2498077 1 BBa_K525998 range2498077 1 1 32 annotation2498082 1 BBa_K1976026 range2498082 1 59 319 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2127788 1 B0034 range2127788 1 21 32 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2129433 1 RBS range2129433 1 24 27 BBa_K1976026 1 BBa_K1976026 Colicin E2 immunity protein (+amber codon) 2016-10-12T11:00:00Z 2016-10-19T02:54:06Z Escherichia coli Immunity protein for Colicin E2 (+amber codon) false false _2443_ 25991 30994 9 false Synthesis feasible by IDT, no amber stop codon for termination false Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Sch&#228;fer, Inka Schr&#246;ter, Viktoria Schuster annotation2497960 1 amber codon range2497960 1 28 30 annotation2497961 1 ImE2 range2497961 1 1 261 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1976026_sequence 1 atggaactgaaacatagtattagtgattagaccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga BBa_K1976033_sequence 1 taatacgactcactatagggaaagaggagaaatactagagaaagaggagaaatactagatggaactgaaacatagtattagtgattagaccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z