BBa_K1976033
1
BBa_K1976033
Inducible generator for colicin E2 immunity protein (+amber codon)
2016-10-12T11:00:00Z
2016-10-19T03:08:11Z
Escherichia coli
Inducible generator for immunity protein for Colicin E2 (+amber codon)
false
false
_2443_
25991
30994
9
false
Synthesis feasible by IDT, no amber stop codon for termination
false
Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster
component2498082
1
BBa_K1976026
component2498077
1
BBa_K525998
component2498079
1
BBa_B0034
annotation2498079
1
BBa_B0034
range2498079
1
41
52
annotation2498077
1
BBa_K525998
range2498077
1
1
32
annotation2498082
1
BBa_K1976026
range2498082
1
59
319
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2127788
1
B0034
range2127788
1
21
32
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2129433
1
RBS
range2129433
1
24
27
BBa_K1976026
1
BBa_K1976026
Colicin E2 immunity protein (+amber codon)
2016-10-12T11:00:00Z
2016-10-19T02:54:06Z
Escherichia coli
Immunity protein for Colicin E2 (+amber codon)
false
false
_2443_
25991
30994
9
false
Synthesis feasible by IDT, no amber stop codon for termination
false
Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster
annotation2497960
1
amber codon
range2497960
1
28
30
annotation2497961
1
ImE2
range2497961
1
1
261
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K1976026_sequence
1
atggaactgaaacatagtattagtgattagaccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga
BBa_K1976033_sequence
1
taatacgactcactatagggaaagaggagaaatactagagaaagaggagaaatactagatggaactgaaacatagtattagtgattagaccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z