LOCUS BBa_K1977004 3180 bp DNA linear 26-Nov-2024 DEFINITION pSB1C3_HemL_HemA ACCESSION BBa_K1977004 VERSION BBa_K1977004.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 229..1643 /label=BBa_K1977002 CDS 1652..3043 /label=BBa_K1977001 stem_loop 3063..3106 /label=stem_loop misc_feature 3052..3131 /label=BBa_B0010 terminator 3052..3131 /label=BBa_B0010 stem_loop 3147..3166 /label=T7 TE misc_feature 3173..3173 /label=stop polyA_site 3167..3180 /label=polya misc_feature 3140..3180 /label=BBa_B0012 terminator 3140..3180 /label=BBa_B0012 terminator 3052..3180 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt gacaattaat 241 catcggctcg tataatgtac tagagttgtg agcggataac aatactagag aaagaggaga 301 aatactagat gctgaaaaac tttaacctgc tgggcggctt tattctgagc aaactgaaca 361 ccacccgcag caaagccgcg tttaaagaag cccagaaata catgccgggt ggcgtgaaca 421 gcccggtgcg tgcctttaaa aacgtgggtg gcgacccgct gtttattgat catggcaagg 481 atgaatacat ctatgacatc gatggcaaca aatacatcga ctatgtgctg agctggggtc 541 cgctgattct gggtcatgcg gatgaccagg tggtggcgga actggataaa gcggtggcga 601 aaggcaccag ctatggcgcc ccgaccctgc aagaaaccga actggcgaaa attgtgaaca 661 aaattatgcc gagcattgaa atgattcgca tggtgagcag cggcaccgaa gcgaccatga 721 gcgcgattcg cctggcccgc ggctacaccc gccgcgaaaa aattgtgaaa tttgtgggca 781 actatcatgg tcatagcgat agcctgctgg tggatgcggg cagcggtctg gcgacctttg 841 gcattaacac cagcccgggc gtgccggatg acctggcgta tgataccctg accgtggcgt 901 acaacgatgt ggatggtgtg aaaaaactgt ttgcggatca tggcgatgaa attgcgtgtg 961 cgattgtgga accggtggcg ggcaacatgg gcgtgattcc gggcacccag gattttctgc 1021 aaaccctgcg cgatgtgacc aaagaacatg gcagcctgct gatttttgat gaagtgatga 1081 gcggctttcg tgccgcgtat catggcgtgc agagcctggt gaacattacc ccggatctga 1141 ccaccctggg caaagtgatt ggtggtggtc tgccggtggg cgcgtttggc ggtcgccgcg 1201 aaattatgga acagattacc ccggctggca gcatttatca tgctggcacc ctgagcggca 1261 acccgctggc gatgaccggt ggcattagca ccctgaaaca gctgaccccg gaagattatg 1321 tggaaatgga taaaaaagtg accgcgctga ccgatggcat taaacaggcg gcggataaat 1381 acggtgtgcc gatgaccgtg catcatgtgg gcaccatgtg gagctatttt tacaacagcg 1441 atagcattaa caactttgat gatgtgaaag cgtgcgatca gaaactgttt gaaaaatgct 1501 tttgggaact gctggcgcat ggcgtgtatg tggcgccgag ccagtttgaa accaacttta 1561 ttagcaccaa acataccgat gaagacattg aaaaaaccat tgccgcgttt gatgcggcgt 1621 ttaacgcggc gaccaaataa taatactaga gaaagaggag aaatactaga tgtgcgtgag 1681 cctgaactat catcagctgc cgattgaact gcgcgaaaaa tttagcttta gcgcggatga 1741 aattccgaaa gcgaaccgcc tgctgaacgc ggaaaaaagc atcctggaaa acctgctgat 1801 tagcacctgc aaccgcacag aaatttatgc ggtggtggat caggtgcata ccggccgcta 1861 ttatattaaa cgctttctgg cggaatggtt tcattatacc gtggaagaat ttaaccagta 1921 tgtgagcatt accaccaaag atgatgcggc gagccatctg ttccgtgtga ttgtgggcct 1981 ggatagcctg atcaaaggcg aaccgcagat tctgggccag atgaaaaacg cgtttcaggt 2041 ggcgaccgat cagggcacca ccggcgtgat cctgaaccat ctgtttcgtc aggcgattac 2101 ctttagcaaa cgcatgcata ccgaatatcg tgtgagcgaa ctggcgcaga gcagcagcca 2161 ggcgggcctg catcagatta aaagcgattt tggcagcctg gaaggcaaaa gcctggcggt 2221 gattggtctg ggccatattg gcaaacatac cgcgtataac gcgagcaaca tgggctttag 2281 caaagtgctg ctgctgaacc gtacagatca gaaagcgaaa cagattgcga acgaactgca 2341 aggcgtggtg gaagcgcgcc cgtttagccg tctggatagc gtgatggcgg aagtggatgc 2401 ggcggtgttt gcggcgagcg tgaaagaacc gctgtatcgc gttaccgatg atcgccagca 2461 ggtgatcgtg gatctgggcg tgccgcgcaa cgtggcgtac gatagcgaaa gcgtgaacta 2521 ttatgatatt gataacctgc atagcattct ggaaaccaac gataccaaaa aaaaccagat 2581 gctgaaccag attgcgaaag aaattccgct ggaagtgaac aacttttatg tgtgggaaaa 2641 acagctgcat attgtgccgg tgattcgcgg cctgcgcgaa catagcctga aaattgaagg 2701 cgaagcgtat gatagcctgc tgcgcaaact gccggaactg aacgcgcatg aacgcaaagt 2761 gattagcaaa catatgaaaa gcattattaa ccagatgatt aaaggcccga tcaaagaaat 2821 taaagaactg agcgtgaccc cgggcgcgac cagcgatatt gatttctttt gcaaaatttt 2881 cggcatggat aacctgaaag tggaaaacca ggaaaacgat cgctaataat actagagcag 2941 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttactact agagttgtca 3001 cgtgagcgga taacaatttc acacaggaaa cataattctt aattactaga gccaggcatc 3061 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3121 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //