LOCUS       BBa_K1977004            3180 bp    DNA     linear       14-Nov-2024
DEFINITION  pSB1C3_HemL_HemA
ACCESSION   BBa_K1977004
VERSION     BBa_K1977004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..1643
                     /label=BBa_K1977002
     CDS             1652..3043
                     /label=BBa_K1977001
     stem_loop       3063..3106
                     /label=stem_loop
     misc_feature    3052..3131
                     /label=BBa_B0010
     terminator      3052..3131
                     /label=BBa_B0010
     stem_loop       3147..3166
                     /label=T7 TE
     misc_feature    3173..3173
                     /label=stop
     polyA_site      3167..3180
                     /label=polya
     misc_feature    3140..3180
                     /label=BBa_B0012
     terminator      3140..3180
                     /label=BBa_B0012
     terminator      3052..3180
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagtt gacaattaat
      241 catcggctcg tataatgtac tagagttgtg agcggataac aatactagag aaagaggaga
      301 aatactagat gctgaaaaac tttaacctgc tgggcggctt tattctgagc aaactgaaca
      361 ccacccgcag caaagccgcg tttaaagaag cccagaaata catgccgggt ggcgtgaaca
      421 gcccggtgcg tgcctttaaa aacgtgggtg gcgacccgct gtttattgat catggcaagg
      481 atgaatacat ctatgacatc gatggcaaca aatacatcga ctatgtgctg agctggggtc
      541 cgctgattct gggtcatgcg gatgaccagg tggtggcgga actggataaa gcggtggcga
      601 aaggcaccag ctatggcgcc ccgaccctgc aagaaaccga actggcgaaa attgtgaaca
      661 aaattatgcc gagcattgaa atgattcgca tggtgagcag cggcaccgaa gcgaccatga
      721 gcgcgattcg cctggcccgc ggctacaccc gccgcgaaaa aattgtgaaa tttgtgggca
      781 actatcatgg tcatagcgat agcctgctgg tggatgcggg cagcggtctg gcgacctttg
      841 gcattaacac cagcccgggc gtgccggatg acctggcgta tgataccctg accgtggcgt
      901 acaacgatgt ggatggtgtg aaaaaactgt ttgcggatca tggcgatgaa attgcgtgtg
      961 cgattgtgga accggtggcg ggcaacatgg gcgtgattcc gggcacccag gattttctgc
     1021 aaaccctgcg cgatgtgacc aaagaacatg gcagcctgct gatttttgat gaagtgatga
     1081 gcggctttcg tgccgcgtat catggcgtgc agagcctggt gaacattacc ccggatctga
     1141 ccaccctggg caaagtgatt ggtggtggtc tgccggtggg cgcgtttggc ggtcgccgcg
     1201 aaattatgga acagattacc ccggctggca gcatttatca tgctggcacc ctgagcggca
     1261 acccgctggc gatgaccggt ggcattagca ccctgaaaca gctgaccccg gaagattatg
     1321 tggaaatgga taaaaaagtg accgcgctga ccgatggcat taaacaggcg gcggataaat
     1381 acggtgtgcc gatgaccgtg catcatgtgg gcaccatgtg gagctatttt tacaacagcg
     1441 atagcattaa caactttgat gatgtgaaag cgtgcgatca gaaactgttt gaaaaatgct
     1501 tttgggaact gctggcgcat ggcgtgtatg tggcgccgag ccagtttgaa accaacttta
     1561 ttagcaccaa acataccgat gaagacattg aaaaaaccat tgccgcgttt gatgcggcgt
     1621 ttaacgcggc gaccaaataa taatactaga gaaagaggag aaatactaga tgtgcgtgag
     1681 cctgaactat catcagctgc cgattgaact gcgcgaaaaa tttagcttta gcgcggatga
     1741 aattccgaaa gcgaaccgcc tgctgaacgc ggaaaaaagc atcctggaaa acctgctgat
     1801 tagcacctgc aaccgcacag aaatttatgc ggtggtggat caggtgcata ccggccgcta
     1861 ttatattaaa cgctttctgg cggaatggtt tcattatacc gtggaagaat ttaaccagta
     1921 tgtgagcatt accaccaaag atgatgcggc gagccatctg ttccgtgtga ttgtgggcct
     1981 ggatagcctg atcaaaggcg aaccgcagat tctgggccag atgaaaaacg cgtttcaggt
     2041 ggcgaccgat cagggcacca ccggcgtgat cctgaaccat ctgtttcgtc aggcgattac
     2101 ctttagcaaa cgcatgcata ccgaatatcg tgtgagcgaa ctggcgcaga gcagcagcca
     2161 ggcgggcctg catcagatta aaagcgattt tggcagcctg gaaggcaaaa gcctggcggt
     2221 gattggtctg ggccatattg gcaaacatac cgcgtataac gcgagcaaca tgggctttag
     2281 caaagtgctg ctgctgaacc gtacagatca gaaagcgaaa cagattgcga acgaactgca
     2341 aggcgtggtg gaagcgcgcc cgtttagccg tctggatagc gtgatggcgg aagtggatgc
     2401 ggcggtgttt gcggcgagcg tgaaagaacc gctgtatcgc gttaccgatg atcgccagca
     2461 ggtgatcgtg gatctgggcg tgccgcgcaa cgtggcgtac gatagcgaaa gcgtgaacta
     2521 ttatgatatt gataacctgc atagcattct ggaaaccaac gataccaaaa aaaaccagat
     2581 gctgaaccag attgcgaaag aaattccgct ggaagtgaac aacttttatg tgtgggaaaa
     2641 acagctgcat attgtgccgg tgattcgcgg cctgcgcgaa catagcctga aaattgaagg
     2701 cgaagcgtat gatagcctgc tgcgcaaact gccggaactg aacgcgcatg aacgcaaagt
     2761 gattagcaaa catatgaaaa gcattattaa ccagatgatt aaaggcccga tcaaagaaat
     2821 taaagaactg agcgtgaccc cgggcgcgac cagcgatatt gatttctttt gcaaaatttt
     2881 cggcatggat aacctgaaag tggaaaacca ggaaaacgat cgctaataat actagagcag
     2941 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttactact agagttgtca
     3001 cgtgagcgga taacaatttc acacaggaaa cataattctt aattactaga gccaggcatc
     3061 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3121 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//