BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1977002
1
BBa_K1977002
HemL glutamate-1-semialdehyde aminotransferase
2016-10-07T11:00:00Z
2016-10-08T08:59:13Z
Lactobacillus reuteri
HemL belongs to the α-family of pyridoxal 5′-phosphate (PLP)-dependent enzymes and can be classified structurally and mechanistically as an aminotransferase. However, in contrast to other aminotransferases GSAM functions as aminomutase acting on a single substrate molecule
false
false
_2444_
27435
27435
9
false
Biobrick compatible
false
Amritpal Singh
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961227
1
start
range1961227
1
173
173
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1977004
1
BBa_K1977004
pSB1C3_HemL_HemA
2016-10-07T11:00:00Z
2016-10-08T09:32:30Z
No
Combination of the HemL and HemA coding parts in order to build the our final construct.
false
false
_2444_
27435
27435
9
false
We had LacI, RBS, HemL, HemA and Double terminator in a pSB1C3 plasmid
false
Amritpal Singh
component2487987
1
BBa_R0010
component2487996
1
BBa_K1977002
component2487995
1
BBa_B0034
component2487997
1
BBa_K1977001
component2488004
1
BBa_B0015
annotation2487996
1
BBa_K1977002
range2487996
1
229
1643
annotation2487995
1
BBa_B0034
range2487995
1
209
220
annotation2488004
1
BBa_B0015
range2488004
1
3052
3180
annotation2487997
1
BBa_K1977001
range2487997
1
1652
3043
annotation2487987
1
BBa_R0010
range2487987
1
1
200
BBa_K1977001
1
BBa_K1977001
HemA
2016-10-07T11:00:00Z
2016-10-08T08:50:39Z
[Lactobacillus reuteri]
HemA glutamyl-tRNA reductase functions as a homodimeric, NADPH-dependent protein. The large tRNA substrate is recognized by its overall shape and NADPH binds to the enzyme with all its major determinants.
false
false
_2444_
27435
27435
9
false
None
false
Amritpal Singh
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1977002_sequence
1
ttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgctgaaaaactttaacctgctgggcggctttattctgagcaaactgaacaccacccgcagcaaagccgcgtttaaagaagcccagaaatacatgccgggtggcgtgaacagcccggtgcgtgcctttaaaaacgtgggtggcgacccgctgtttattgatcatggcaaggatgaatacatctatgacatcgatggcaacaaatacatcgactatgtgctgagctggggtccgctgattctgggtcatgcggatgaccaggtggtggcggaactggataaagcggtggcgaaaggcaccagctatggcgccccgaccctgcaagaaaccgaactggcgaaaattgtgaacaaaattatgccgagcattgaaatgattcgcatggtgagcagcggcaccgaagcgaccatgagcgcgattcgcctggcccgcggctacacccgccgcgaaaaaattgtgaaatttgtgggcaactatcatggtcatagcgatagcctgctggtggatgcgggcagcggtctggcgacctttggcattaacaccagcccgggcgtgccggatgacctggcgtatgataccctgaccgtggcgtacaacgatgtggatggtgtgaaaaaactgtttgcggatcatggcgatgaaattgcgtgtgcgattgtggaaccggtggcgggcaacatgggcgtgattccgggcacccaggattttctgcaaaccctgcgcgatgtgaccaaagaacatggcagcctgctgatttttgatgaagtgatgagcggctttcgtgccgcgtatcatggcgtgcagagcctggtgaacattaccccggatctgaccaccctgggcaaagtgattggtggtggtctgccggtgggcgcgtttggcggtcgccgcgaaattatggaacagattaccccggctggcagcatttatcatgctggcaccctgagcggcaacccgctggcgatgaccggtggcattagcaccctgaaacagctgaccccggaagattatgtggaaatggataaaaaagtgaccgcgctgaccgatggcattaaacaggcggcggataaatacggtgtgccgatgaccgtgcatcatgtgggcaccatgtggagctatttttacaacagcgatagcattaacaactttgatgatgtgaaagcgtgcgatcagaaactgtttgaaaaatgcttttgggaactgctggcgcatggcgtgtatgtggcgccgagccagtttgaaaccaactttattagcaccaaacataccgatgaagacattgaaaaaaccattgccgcgtttgatgcggcgtttaacgcggcgaccaaataataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1977001_sequence
1
aaagaggagaaatactagatgtgcgtgagcctgaactatcatcagctgccgattgaactgcgcgaaaaatttagctttagcgcggatgaaattccgaaagcgaaccgcctgctgaacgcggaaaaaagcatcctggaaaacctgctgattagcacctgcaaccgcacagaaatttatgcggtggtggatcaggtgcataccggccgctattatattaaacgctttctggcggaatggtttcattataccgtggaagaatttaaccagtatgtgagcattaccaccaaagatgatgcggcgagccatctgttccgtgtgattgtgggcctggatagcctgatcaaaggcgaaccgcagattctgggccagatgaaaaacgcgtttcaggtggcgaccgatcagggcaccaccggcgtgatcctgaaccatctgtttcgtcaggcgattacctttagcaaacgcatgcataccgaatatcgtgtgagcgaactggcgcagagcagcagccaggcgggcctgcatcagattaaaagcgattttggcagcctggaaggcaaaagcctggcggtgattggtctgggccatattggcaaacataccgcgtataacgcgagcaacatgggctttagcaaagtgctgctgctgaaccgtacagatcagaaagcgaaacagattgcgaacgaactgcaaggcgtggtggaagcgcgcccgtttagccgtctggatagcgtgatggcggaagtggatgcggcggtgtttgcggcgagcgtgaaagaaccgctgtatcgcgttaccgatgatcgccagcaggtgatcgtggatctgggcgtgccgcgcaacgtggcgtacgatagcgaaagcgtgaactattatgatattgataacctgcatagcattctggaaaccaacgataccaaaaaaaaccagatgctgaaccagattgcgaaagaaattccgctggaagtgaacaacttttatgtgtgggaaaaacagctgcatattgtgccggtgattcgcggcctgcgcgaacatagcctgaaaattgaaggcgaagcgtatgatagcctgctgcgcaaactgccggaactgaacgcgcatgaacgcaaagtgattagcaaacatatgaaaagcattattaaccagatgattaaaggcccgatcaaagaaattaaagaactgagcgtgaccccgggcgcgaccagcgatattgatttcttttgcaaaattttcggcatggataacctgaaagtggaaaaccaggaaaacgatcgctaataatactagagcaggaaacagctatgaccatgattacggattcactggccgtcgttttactactagagttgtcacgtgagcggataacaatttcacacaggaaacataattcttaat
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1977004_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgctgaaaaactttaacctgctgggcggctttattctgagcaaactgaacaccacccgcagcaaagccgcgtttaaagaagcccagaaatacatgccgggtggcgtgaacagcccggtgcgtgcctttaaaaacgtgggtggcgacccgctgtttattgatcatggcaaggatgaatacatctatgacatcgatggcaacaaatacatcgactatgtgctgagctggggtccgctgattctgggtcatgcggatgaccaggtggtggcggaactggataaagcggtggcgaaaggcaccagctatggcgccccgaccctgcaagaaaccgaactggcgaaaattgtgaacaaaattatgccgagcattgaaatgattcgcatggtgagcagcggcaccgaagcgaccatgagcgcgattcgcctggcccgcggctacacccgccgcgaaaaaattgtgaaatttgtgggcaactatcatggtcatagcgatagcctgctggtggatgcgggcagcggtctggcgacctttggcattaacaccagcccgggcgtgccggatgacctggcgtatgataccctgaccgtggcgtacaacgatgtggatggtgtgaaaaaactgtttgcggatcatggcgatgaaattgcgtgtgcgattgtggaaccggtggcgggcaacatgggcgtgattccgggcacccaggattttctgcaaaccctgcgcgatgtgaccaaagaacatggcagcctgctgatttttgatgaagtgatgagcggctttcgtgccgcgtatcatggcgtgcagagcctggtgaacattaccccggatctgaccaccctgggcaaagtgattggtggtggtctgccggtgggcgcgtttggcggtcgccgcgaaattatggaacagattaccccggctggcagcatttatcatgctggcaccctgagcggcaacccgctggcgatgaccggtggcattagcaccctgaaacagctgaccccggaagattatgtggaaatggataaaaaagtgaccgcgctgaccgatggcattaaacaggcggcggataaatacggtgtgccgatgaccgtgcatcatgtgggcaccatgtggagctatttttacaacagcgatagcattaacaactttgatgatgtgaaagcgtgcgatcagaaactgtttgaaaaatgcttttgggaactgctggcgcatggcgtgtatgtggcgccgagccagtttgaaaccaactttattagcaccaaacataccgatgaagacattgaaaaaaccattgccgcgtttgatgcggcgtttaacgcggcgaccaaataataatactagagaaagaggagaaatactagatgtgcgtgagcctgaactatcatcagctgccgattgaactgcgcgaaaaatttagctttagcgcggatgaaattccgaaagcgaaccgcctgctgaacgcggaaaaaagcatcctggaaaacctgctgattagcacctgcaaccgcacagaaatttatgcggtggtggatcaggtgcataccggccgctattatattaaacgctttctggcggaatggtttcattataccgtggaagaatttaaccagtatgtgagcattaccaccaaagatgatgcggcgagccatctgttccgtgtgattgtgggcctggatagcctgatcaaaggcgaaccgcagattctgggccagatgaaaaacgcgtttcaggtggcgaccgatcagggcaccaccggcgtgatcctgaaccatctgtttcgtcaggcgattacctttagcaaacgcatgcataccgaatatcgtgtgagcgaactggcgcagagcagcagccaggcgggcctgcatcagattaaaagcgattttggcagcctggaaggcaaaagcctggcggtgattggtctgggccatattggcaaacataccgcgtataacgcgagcaacatgggctttagcaaagtgctgctgctgaaccgtacagatcagaaagcgaaacagattgcgaacgaactgcaaggcgtggtggaagcgcgcccgtttagccgtctggatagcgtgatggcggaagtggatgcggcggtgtttgcggcgagcgtgaaagaaccgctgtatcgcgttaccgatgatcgccagcaggtgatcgtggatctgggcgtgccgcgcaacgtggcgtacgatagcgaaagcgtgaactattatgatattgataacctgcatagcattctggaaaccaacgataccaaaaaaaaccagatgctgaaccagattgcgaaagaaattccgctggaagtgaacaacttttatgtgtgggaaaaacagctgcatattgtgccggtgattcgcggcctgcgcgaacatagcctgaaaattgaaggcgaagcgtatgatagcctgctgcgcaaactgccggaactgaacgcgcatgaacgcaaagtgattagcaaacatatgaaaagcattattaaccagatgattaaaggcccgatcaaagaaattaaagaactgagcgtgaccccgggcgcgaccagcgatattgatttcttttgcaaaattttcggcatggataacctgaaagtggaaaaccaggaaaacgatcgctaataatactagagcaggaaacagctatgaccatgattacggattcactggccgtcgttttactactagagttgtcacgtgagcggataacaatttcacacaggaaacataattcttaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z