BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1977002 1 BBa_K1977002 HemL glutamate-1-semialdehyde aminotransferase 2016-10-07T11:00:00Z 2016-10-08T08:59:13Z Lactobacillus reuteri HemL belongs to the &#945;-family of pyridoxal 5&#8242;-phosphate (PLP)-dependent enzymes and can be classified structurally and mechanistically as an aminotransferase. However, in contrast to other aminotransferases GSAM functions as aminomutase acting on a single substrate molecule false false _2444_ 27435 27435 9 false Biobrick compatible false Amritpal Singh BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1977005 1 BBa_K1977005 HemL_HemA_HemF 2016-10-07T11:00:00Z 2016-10-08T09:47:35Z None This is the final construct compiled of HemL, HemA, HemF. false false _2444_ 27435 27435 9 false LacI, RBS, HemL, HemA, HemF, Double terminator. false Amritpal Singh component2488014 1 BBa_K1977002 component2488013 1 BBa_B0034 component2488016 1 BBa_K1977003 component2488005 1 BBa_R0010 component2488023 1 BBa_B0015 component2488015 1 BBa_K1977001 annotation2488014 1 BBa_K1977002 range2488014 1 229 1643 annotation2488005 1 BBa_R0010 range2488005 1 1 200 annotation2488015 1 BBa_K1977001 range2488015 1 1652 3043 annotation2488013 1 BBa_B0034 range2488013 1 209 220 annotation2488016 1 BBa_K1977003 range2488016 1 3052 4067 annotation2488023 1 BBa_B0015 range2488023 1 4076 4204 BBa_K1977001 1 BBa_K1977001 HemA 2016-10-07T11:00:00Z 2016-10-08T08:50:39Z [Lactobacillus reuteri] HemA glutamyl-tRNA reductase functions as a homodimeric, NADPH-dependent protein. The large tRNA substrate is recognized by its overall shape and NADPH binds to the enzyme with all its major determinants. false false _2444_ 27435 27435 9 false None false Amritpal Singh BBa_K1977003 1 BBa_K1977003 HemF coproporphyrinogen III oxidase 2016-10-07T11:00:00Z 2016-10-08T09:11:22Z Rhodobacter sphaeroides Responsible for the conversion of coproporphyrinogen III into protoporphyrinogen IX requires the oxidative decarboxylation of the propionate side chains on rings A and B of the tetrapyrrole to yield the corresponding vinyl groups false false _2444_ 27435 27435 9 false Biobrick compatible false Amritpal Singh BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1977002_sequence 1 ttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgctgaaaaactttaacctgctgggcggctttattctgagcaaactgaacaccacccgcagcaaagccgcgtttaaagaagcccagaaatacatgccgggtggcgtgaacagcccggtgcgtgcctttaaaaacgtgggtggcgacccgctgtttattgatcatggcaaggatgaatacatctatgacatcgatggcaacaaatacatcgactatgtgctgagctggggtccgctgattctgggtcatgcggatgaccaggtggtggcggaactggataaagcggtggcgaaaggcaccagctatggcgccccgaccctgcaagaaaccgaactggcgaaaattgtgaacaaaattatgccgagcattgaaatgattcgcatggtgagcagcggcaccgaagcgaccatgagcgcgattcgcctggcccgcggctacacccgccgcgaaaaaattgtgaaatttgtgggcaactatcatggtcatagcgatagcctgctggtggatgcgggcagcggtctggcgacctttggcattaacaccagcccgggcgtgccggatgacctggcgtatgataccctgaccgtggcgtacaacgatgtggatggtgtgaaaaaactgtttgcggatcatggcgatgaaattgcgtgtgcgattgtggaaccggtggcgggcaacatgggcgtgattccgggcacccaggattttctgcaaaccctgcgcgatgtgaccaaagaacatggcagcctgctgatttttgatgaagtgatgagcggctttcgtgccgcgtatcatggcgtgcagagcctggtgaacattaccccggatctgaccaccctgggcaaagtgattggtggtggtctgccggtgggcgcgtttggcggtcgccgcgaaattatggaacagattaccccggctggcagcatttatcatgctggcaccctgagcggcaacccgctggcgatgaccggtggcattagcaccctgaaacagctgaccccggaagattatgtggaaatggataaaaaagtgaccgcgctgaccgatggcattaaacaggcggcggataaatacggtgtgccgatgaccgtgcatcatgtgggcaccatgtggagctatttttacaacagcgatagcattaacaactttgatgatgtgaaagcgtgcgatcagaaactgtttgaaaaatgcttttgggaactgctggcgcatggcgtgtatgtggcgccgagccagtttgaaaccaactttattagcaccaaacataccgatgaagacattgaaaaaaccattgccgcgtttgatgcggcgtttaacgcggcgaccaaataataa BBa_K1977003_sequence 1 ttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgaacgatgccatggatggccagaaagcgcgcgcggcggattggtttcgcgaactgcgcaaccgcattctggcggcgtttgaaggcctggaagacgcggcagcgggcggtagcccgaccgatccagcgccaggtcgctttgaagtgagcgaaaccacccgcgaagcgggtggcggtggcctgatgagcgtgctgcgcggcggtcgcgtgtttgaaaaagtgggcgtgaacgtgagcaccgttcatggtaccctggcagcgcgcgcccagcaggccatggcggcccgtggtgttccaggtatggcggaagatccgcgcttttgggcgagcggcattagcctggtggcccatatgcagaacccgcacgcgccagcggttcatatgaacacccgtatgttttggaccccgcacgcctggtggtttggtggtggcagcgatctgaacccgtgcctggatcatccggaagataccgaacattttcacgcgaccctgcgccgcggctgtgaagcgcatggcgcggattattatccgcgctttaaagcgtgggcggatgaatatttctttatcccgcatcgcggtcgcgcgcgtggtgttggcggtatcttttatgatgatctgaacagcggcgattgggaagcggattttgcgtttacccgcgcggtgggtgaagcgtttctgccggcgtttgtgccgctggtggaacgccgcatgggcaccccgtggaccgaagccgatcgcgaagcgcagctgattcatcgcggcctgtatgcggaatataacctggtgtatgatcgcggcaccaaatttggcctggaaaccggccatgatgcgaacgcggtgctgatgagcctgccgccgctggcgaaatggatttaataatactagagttattgctcagcggtggcagcagccaactcagcttcctttcgggctttg BBa_B0034_sequence 1 aaagaggagaaa BBa_K1977005_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgctgaaaaactttaacctgctgggcggctttattctgagcaaactgaacaccacccgcagcaaagccgcgtttaaagaagcccagaaatacatgccgggtggcgtgaacagcccggtgcgtgcctttaaaaacgtgggtggcgacccgctgtttattgatcatggcaaggatgaatacatctatgacatcgatggcaacaaatacatcgactatgtgctgagctggggtccgctgattctgggtcatgcggatgaccaggtggtggcggaactggataaagcggtggcgaaaggcaccagctatggcgccccgaccctgcaagaaaccgaactggcgaaaattgtgaacaaaattatgccgagcattgaaatgattcgcatggtgagcagcggcaccgaagcgaccatgagcgcgattcgcctggcccgcggctacacccgccgcgaaaaaattgtgaaatttgtgggcaactatcatggtcatagcgatagcctgctggtggatgcgggcagcggtctggcgacctttggcattaacaccagcccgggcgtgccggatgacctggcgtatgataccctgaccgtggcgtacaacgatgtggatggtgtgaaaaaactgtttgcggatcatggcgatgaaattgcgtgtgcgattgtggaaccggtggcgggcaacatgggcgtgattccgggcacccaggattttctgcaaaccctgcgcgatgtgaccaaagaacatggcagcctgctgatttttgatgaagtgatgagcggctttcgtgccgcgtatcatggcgtgcagagcctggtgaacattaccccggatctgaccaccctgggcaaagtgattggtggtggtctgccggtgggcgcgtttggcggtcgccgcgaaattatggaacagattaccccggctggcagcatttatcatgctggcaccctgagcggcaacccgctggcgatgaccggtggcattagcaccctgaaacagctgaccccggaagattatgtggaaatggataaaaaagtgaccgcgctgaccgatggcattaaacaggcggcggataaatacggtgtgccgatgaccgtgcatcatgtgggcaccatgtggagctatttttacaacagcgatagcattaacaactttgatgatgtgaaagcgtgcgatcagaaactgtttgaaaaatgcttttgggaactgctggcgcatggcgtgtatgtggcgccgagccagtttgaaaccaactttattagcaccaaacataccgatgaagacattgaaaaaaccattgccgcgtttgatgcggcgtttaacgcggcgaccaaataataatactagagaaagaggagaaatactagatgtgcgtgagcctgaactatcatcagctgccgattgaactgcgcgaaaaatttagctttagcgcggatgaaattccgaaagcgaaccgcctgctgaacgcggaaaaaagcatcctggaaaacctgctgattagcacctgcaaccgcacagaaatttatgcggtggtggatcaggtgcataccggccgctattatattaaacgctttctggcggaatggtttcattataccgtggaagaatttaaccagtatgtgagcattaccaccaaagatgatgcggcgagccatctgttccgtgtgattgtgggcctggatagcctgatcaaaggcgaaccgcagattctgggccagatgaaaaacgcgtttcaggtggcgaccgatcagggcaccaccggcgtgatcctgaaccatctgtttcgtcaggcgattacctttagcaaacgcatgcataccgaatatcgtgtgagcgaactggcgcagagcagcagccaggcgggcctgcatcagattaaaagcgattttggcagcctggaaggcaaaagcctggcggtgattggtctgggccatattggcaaacataccgcgtataacgcgagcaacatgggctttagcaaagtgctgctgctgaaccgtacagatcagaaagcgaaacagattgcgaacgaactgcaaggcgtggtggaagcgcgcccgtttagccgtctggatagcgtgatggcggaagtggatgcggcggtgtttgcggcgagcgtgaaagaaccgctgtatcgcgttaccgatgatcgccagcaggtgatcgtggatctgggcgtgccgcgcaacgtggcgtacgatagcgaaagcgtgaactattatgatattgataacctgcatagcattctggaaaccaacgataccaaaaaaaaccagatgctgaaccagattgcgaaagaaattccgctggaagtgaacaacttttatgtgtgggaaaaacagctgcatattgtgccggtgattcgcggcctgcgcgaacatagcctgaaaattgaaggcgaagcgtatgatagcctgctgcgcaaactgccggaactgaacgcgcatgaacgcaaagtgattagcaaacatatgaaaagcattattaaccagatgattaaaggcccgatcaaagaaattaaagaactgagcgtgaccccgggcgcgaccagcgatattgatttcttttgcaaaattttcggcatggataacctgaaagtggaaaaccaggaaaacgatcgctaataatactagagcaggaaacagctatgaccatgattacggattcactggccgtcgttttactactagagttgtcacgtgagcggataacaatttcacacaggaaacataattcttaattactagagttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatgaacgatgccatggatggccagaaagcgcgcgcggcggattggtttcgcgaactgcgcaaccgcattctggcggcgtttgaaggcctggaagacgcggcagcgggcggtagcccgaccgatccagcgccaggtcgctttgaagtgagcgaaaccacccgcgaagcgggtggcggtggcctgatgagcgtgctgcgcggcggtcgcgtgtttgaaaaagtgggcgtgaacgtgagcaccgttcatggtaccctggcagcgcgcgcccagcaggccatggcggcccgtggtgttccaggtatggcggaagatccgcgcttttgggcgagcggcattagcctggtggcccatatgcagaacccgcacgcgccagcggttcatatgaacacccgtatgttttggaccccgcacgcctggtggtttggtggtggcagcgatctgaacccgtgcctggatcatccggaagataccgaacattttcacgcgaccctgcgccgcggctgtgaagcgcatggcgcggattattatccgcgctttaaagcgtgggcggatgaatatttctttatcccgcatcgcggtcgcgcgcgtggtgttggcggtatcttttatgatgatctgaacagcggcgattgggaagcggattttgcgtttacccgcgcggtgggtgaagcgtttctgccggcgtttgtgccgctggtggaacgccgcatgggcaccccgtggaccgaagccgatcgcgaagcgcagctgattcatcgcggcctgtatgcggaatataacctggtgtatgatcgcggcaccaaatttggcctggaaaccggccatgatgcgaacgcggtgctgatgagcctgccgccgctggcgaaatggatttaataatactagagttattgctcagcggtggcagcagccaactcagcttcctttcgggctttgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1977001_sequence 1 aaagaggagaaatactagatgtgcgtgagcctgaactatcatcagctgccgattgaactgcgcgaaaaatttagctttagcgcggatgaaattccgaaagcgaaccgcctgctgaacgcggaaaaaagcatcctggaaaacctgctgattagcacctgcaaccgcacagaaatttatgcggtggtggatcaggtgcataccggccgctattatattaaacgctttctggcggaatggtttcattataccgtggaagaatttaaccagtatgtgagcattaccaccaaagatgatgcggcgagccatctgttccgtgtgattgtgggcctggatagcctgatcaaaggcgaaccgcagattctgggccagatgaaaaacgcgtttcaggtggcgaccgatcagggcaccaccggcgtgatcctgaaccatctgtttcgtcaggcgattacctttagcaaacgcatgcataccgaatatcgtgtgagcgaactggcgcagagcagcagccaggcgggcctgcatcagattaaaagcgattttggcagcctggaaggcaaaagcctggcggtgattggtctgggccatattggcaaacataccgcgtataacgcgagcaacatgggctttagcaaagtgctgctgctgaaccgtacagatcagaaagcgaaacagattgcgaacgaactgcaaggcgtggtggaagcgcgcccgtttagccgtctggatagcgtgatggcggaagtggatgcggcggtgtttgcggcgagcgtgaaagaaccgctgtatcgcgttaccgatgatcgccagcaggtgatcgtggatctgggcgtgccgcgcaacgtggcgtacgatagcgaaagcgtgaactattatgatattgataacctgcatagcattctggaaaccaacgataccaaaaaaaaccagatgctgaaccagattgcgaaagaaattccgctggaagtgaacaacttttatgtgtgggaaaaacagctgcatattgtgccggtgattcgcggcctgcgcgaacatagcctgaaaattgaaggcgaagcgtatgatagcctgctgcgcaaactgccggaactgaacgcgcatgaacgcaaagtgattagcaaacatatgaaaagcattattaaccagatgattaaaggcccgatcaaagaaattaaagaactgagcgtgaccccgggcgcgaccagcgatattgatttcttttgcaaaattttcggcatggataacctgaaagtggaaaaccaggaaaacgatcgctaataatactagagcaggaaacagctatgaccatgattacggattcactggccgtcgttttactactagagttgtcacgtgagcggataacaatttcacacaggaaacataattcttaat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z