BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1977006
1
BBa_K1977006
HemD uroporphyrinogen III methyltransferase
2016-10-07T11:00:00Z
2016-10-08T09:51:54Z
Rhodobacter sphaeroides
Catalyses the stepwise decarboxylation of the four acetic acid chains to yield the corresponding methyl groups of coproporphyrinogen III
false
false
_2444_
27435
27435
9
false
None
false
Amritpal Singh
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961227
1
start
range1961227
1
173
173
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1977007
1
BBa_K1977007
HemD_RhtA
2016-10-07T11:00:00Z
2016-10-08T10:04:30Z
None
Final Construct for HemD_RhtA.
false
false
_2444_
27435
27435
9
false
LacI, RBS, HemD, RhtA, Double terminator
false
Amritpal Singh
component2488195
1
BBa_B0034
component2488204
1
BBa_B0015
component2488197
1
BBa_K1977006
component2488187
1
BBa_R0010
component2488196
1
BBa_K1977000
annotation2488196
1
BBa_K1977000
range2488196
1
229
1195
annotation2488204
1
BBa_B0015
range2488204
1
2039
2167
annotation2488187
1
BBa_R0010
range2488187
1
1
200
annotation2488197
1
BBa_K1977006
range2488197
1
1204
2030
annotation2488195
1
BBa_B0034
range2488195
1
209
220
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1977000
1
BBa_K1977000
RhtA
2016-10-07T11:00:00Z
2016-10-08T09:57:04Z
TTGACAATTAATCATCGGCTCGTATAATGTACTAGAGTTGTGAGCGGATAACAATACTAGAGAAAGAGGAGAAATACTAGATGCTGAAAAACTTTAACCTGCTGGGCGGCTTTATTCTGAGCAAACTGAACACCACCCGCAGCAAAGCCGCGTTTAAAGAAGCCCAGAAATACATGCCGGGTGGCGTGAACAGCCCGGTGCGTGCCTTTAAAAACGTGGGTGGCGACCCGCTGTTTATTGATCATGGCAAGGATGAATACATCTATGACATCGATGGCAACAAATACATCGACTATGTGCTGAGCTGGGGTCCGCTGATTCTGGGTCATGCGGATGACCAGGTGGTGGCGGAACTGGATAAAGCGGTGGCGAAAGGCACCAGCTATGGCGCCCCGACCCTGCAAGAAACCGAACTGGCGAAAATTGTGAACAAAATTATGCCGAGCATTGAAATGATTCGCATGGTGAGCAGCGGCACCGAAGCGACCATGAGCGCGATTCGCCTGGCCCGCGGCTACACCCGCCGCGAAAAAATTGTGAAATTTGTGGGCAACTATCATGGTCATAGCGATAGCCTGCTGGTGGATGCGGGCAGCGGTCTGGCGACCTTTGGCATTAACACCAGCCCGGGCGTGCCGGATGACCTGGCGTATGATACCCTGACCGTGGCGTACAACGATGTGGATGGTGTGAAAAAACTGTTTGCGGATCATGGCGATGAAATTGCGTGTGCGATTGTGGAACCGGTGGCGGGCAACATGGGCGTGATTCCGGGCACCCAGGATTTTCTGCAAACCCTGCGCGATGTGACCAAAGAACATGGCAGCCTGCTGATTTTTGATGAAGTGATGAGCGGCTTTCGTGCCGCGTATCATGGCGTGCAGAGCCTGGTGAACATTACCCCGGATCTGACCACCCTGGGCAAAGTGATTGGTGGTGGTCTGCCGGTGGGCGCGTTTGGCGGTCGCCGCGAAATTATGGAACAGATTACCCCGGCTGGCAGCATTTATCATGCTGGCACCCTGAGCGGCAACCCGCTGGCGATGACCGGTGGCATTAGCACCCTGAAACAGCTGACCCCGGAAGATTATGTGGAAATGGATAAAAAAGTGACCGCGCTGACCGATGGCATTAAACAGGCGGCGGATAAATACGGTGTGCCGATGACCGTGCATCATGTGGGCACCATGTGGAGCTATTTTTACAACAGCGATAGCATTAACAACTTTGATGATGTGAAAGCGTGCGATCAGAAACTGTTTGAAAAATGCTTTTGGGAACTGCTGGCGCATGGCGTGTATGTGGCGCCGAGCCAGTTTGAAACCAACTTTATTAGCACCAAACATACCGATGAAGACATTGAAAAAACCATTGCCGCGTTTGATGCGGCGTTTAACGCGGCGACCAAATAATAA
Lactobacillus reuteri
The glutamate-1-semialdehyde-2,1-aminomutase belongs to the family of isomerases. It is also called glutamate-1-semialdehyde aminotransferase. This enzyme participates in the porphyrin and chlorophyll biosynthesis where it catalyses L-glutamate 1-semialdehyde (GSA) to 5-aminolevulinate in the C5 pathway.
false
false
_2444_
27435
30384
9
false
When designing the sequence we added a tac promoter, lac operator and a RBS. It is in BioBrick standard format.
false
Camila Gaspar
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1977007_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagaaagaggagaaatactagatgccgggtccgagccgcaaagcggcggcgtggctgccgatcctggttatcctgattgcgatgaccagcattcaaagcggcgcaagcctggcgaaatctctgtttccgctggttggcgcgccgggcgtgaccgcattgcgtctggcgttgggtacgctgattctggttgtggtgttcaaaccgtggcgcctgcgctttagcccggcgcaacgcgtgccgctgttgctgtatggtttggccctgggtgcgatgaactatctgttctatctgagcctgcaacgcattccgttgggcgttgcggtggccctggagtttaccggtccgcttgcggtggcactgtttggcagccgccgcccgcttgattttgtgtgggttgccttggcgattctgggcctgtggtatttgttgccgttgggtcaaaacgttgcgcaagtggatctgaccggtgcgttgtttgcgttgggtgcgggtgcgtgctgggcggtgtatattctgaccggccaacgcgcgggcgaagaacatggtccggcgaccgttgcgatgggttctctgattgcgtctctggtgtttgttccgctgggtatggcgcaggcgaccgataccctgtggcagtggagcctgttgccgatgggcctgggcattgcgatcctgagcaccgcgctgccgtatagcctggaaatgatggccctgacccgcctgccgacccgcacctttggcaccctgatgtctctggaaccggcgttggcggcgctgagcggcatggtgtttctgggcgaaacgctgaaactgagccaaaccttggcgctgggcgcgattattattgcgagcatgggcgcgaccctgaccatgccgcgccagagcaaaattgaacaagttgatattaactaataatactagagttattgctcatgcggtggcagcagccaactcagcttcctttcgggctttgtactagagttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatggcgccgcagagccgtccgctgctgccggcggtgctgctgacccgtccggaagcgcagggtgcgcgctttgcggtggcgctgcgtgaagcgctgggtccggtgcgtctggtgaccagcccgctgatggtgccggaatttctgagcccgccgattgcgctgcgcccagaagcgctgatttttaccagcgaaaccggcgtggaaggctttcgtcgtctggctgcgccaggtctggcgggtgtgggccgtgcatggtgcgtgggtaaccgtaccgcacgtgcagcgcgtgcagcgggcctggatgcgcagagcgcggaaggcgatgcggaagcactgattgcggcgattgcagcggcaggtgaaagcggtccgctgctgcatctgcgtggcgcggaaagccgtggcgaagtggcgccgcgtctggcagcgcagggcctggtggcggaagaagcggtggtgtatgatcagcgtccgcagccgctgctgccggaagcgctggcgctgctggcgggtggtgcaccggtgctggcgccgctgtttagcccgcgtaccgcgcgtctgctgggtctggaactggcgcgtgcgggtgtggcaccgcagctgtgggtggcggcaatgagcccggcggtggcagaagccgcgggtgcactgagcccggcacgtctgcgtgttgcagcacgtccggatgcagaagcgctgctggacgcggtgaaagcgctgctggatgcggaagcggatgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1977006_sequence
1
ttgacaattaatcatcggctcgtataatgtactagagttgtgagcggataacaatactagagaaagaggagaaatactagatggcgccgcagagccgtccgctgctgccggcggtgctgctgacccgtccggaagcgcagggtgcgcgctttgcggtggcgctgcgtgaagcgctgggtccggtgcgtctggtgaccagcccgctgatggtgccggaatttctgagcccgccgattgcgctgcgcccagaagcgctgatttttaccagcgaaaccggcgtggaaggctttcgtcgtctggctgcgccaggtctggcgggtgtgggccgtgcatggtgcgtgggtaaccgtaccgcacgtgcagcgcgtgcagcgggcctggatgcgcagagcgcggaaggcgatgcggaagcactgattgcggcgattgcagcggcaggtgaaagcggtccgctgctgcatctgcgtggcgcggaaagccgtggcgaagtggcgccgcgtctggcagcgcagggcctggtggcggaagaagcggtggtgtatgatcagcgtccgcagccgctgctgccggaagcgctggcgctgctggcgggtggtgcaccggtgctggcgccgctgtttagcccgcgtaccgcgcgtctgctgggtctggaactggcgcgtgcgggtgtggcaccgcagctgtgggtggcggcaatgagcccggcggtggcagaagccgcgggtgcactgagcccggcacgtctgcgtgttgcagcacgtccggatgcagaagcgctgctggacgcggtgaaagcgctgctggatgcggaagcggatgcgtaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1977000_sequence
1
aaagaggagaaatactagatgccgggtccgagccgcaaagcggcggcgtggctgccgatcctggttatcctgattgcgatgaccagcattcaaagcggcgcaagcctggcgaaatctctgtttccgctggttggcgcgccgggcgtgaccgcattgcgtctggcgttgggtacgctgattctggttgtggtgttcaaaccgtggcgcctgcgctttagcccggcgcaacgcgtgccgctgttgctgtatggtttggccctgggtgcgatgaactatctgttctatctgagcctgcaacgcattccgttgggcgttgcggtggccctggagtttaccggtccgcttgcggtggcactgtttggcagccgccgcccgcttgattttgtgtgggttgccttggcgattctgggcctgtggtatttgttgccgttgggtcaaaacgttgcgcaagtggatctgaccggtgcgttgtttgcgttgggtgcgggtgcgtgctgggcggtgtatattctgaccggccaacgcgcgggcgaagaacatggtccggcgaccgttgcgatgggttctctgattgcgtctctggtgtttgttccgctgggtatggcgcaggcgaccgataccctgtggcagtggagcctgttgccgatgggcctgggcattgcgatcctgagcaccgcgctgccgtatagcctggaaatgatggccctgacccgcctgccgacccgcacctttggcaccctgatgtctctggaaccggcgttggcggcgctgagcggcatggtgtttctgggcgaaacgctgaaactgagccaaaccttggcgctgggcgcgattattattgcgagcatgggcgcgaccctgaccatgccgcgccagagcaaaattgaacaagttgatattaactaataatactagagttattgctcatgcggtggcagcagccaactcagcttcctttcgggctttg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z