LOCUS BBa_K1979003 1523 bp DNA linear 22-Nov-2024 DEFINITION PBP5 coding device (promoter+PBP5+T7 terminator) ACCESSION BBa_K1979003 VERSION BBa_K1979003.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23101 RBS 58..64 /label=g10-L rbs misc_feature 84..101 /label=6??his tag misc_feature 131..164 /label=T7 tag misc_feature 173..176 /label=start codon misc_feature 1476..1523 /label=T7 Terminator CDS 177..1385 /label=PBP5 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctacgaaata attttgttta actttaagaa 61 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc 121 gcggcagcca tatggctagc atgactggtg gacagcaaat gggtcgcgga tccatgaata 181 ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct 241 ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc 301 agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac 361 agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta 421 tcggccaggc aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg 481 acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg 541 gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg 601 cttgtgtcgc catggccgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga 661 acagctacgt taacgcactg ggcctgaaaa atacccactt ccagacggta catggtctgg 721 atgctgatgg tcagtacagc tccgcgcgag atatggcgct gatcggccag gcattgatcc 781 gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc 841 gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa 901 ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc 961 gcttgatttc tgcggtaatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa 1021 aactgctaac ctggggcttc cgtttctttg aaaccgttaa cccactgaaa gtaggtaaag 1081 agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggggttgata 1141 aagacgtgta cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc 1201 tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact 1261 tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg 1321 aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg 1381 gttaactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg 1441 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 1501 aacgggtctt gaggggtttt ttg //