BBa_K1979003 1 BBa_K1979003 PBP5 coding device (promoter+PBP5+T7 terminator) 2016-08-25T11:00:00Z 2016-08-26T02:45:54Z The PBP5 and terminator sequences are retrieved form GenBank, and the promoter sequence comes from the part BBa_J23101. This device codes for Penicillin binding protein 5. The coding sequence is tagged by 6??his and T7, and it is controlled by a constitutive promoter BBa_J23101 and a T7 terminator. false false _2446_ 29797 29797 9 false Histidine tag is added for affinity purification to harvest the proteins. false MA Xinyi annotation2481645 1 start codon range2481645 1 173 176 annotation2481641 1 J23101 range2481641 1 1 35 annotation2481647 1 PBP5 range2481647 1 177 1385 annotation2481646 1 T7 Terminator range2481646 1 1476 1523 annotation2481644 1 T7 tag range2481644 1 131 164 annotation2481642 1 g10-L rbs range2481642 1 58 64 annotation2481643 1 6??his tag range2481643 1 84 101 BBa_K1979003_sequence 1 tttacagctagctcagtcctaggtattatgctacgaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgactggtggacagcaaatgggtcgcggatccatgaataccattttttccgctcgtatcatgaagcgcctggcgctcaccacggctctttgcacagcctttatctctgctgcacatgccgatgacctgaatatcaaaactatgatcccgggtgtaccgcagatcgatgcggagtcctacatcctgattgactataactccggcaaagtgctcgccgaacagaacgcagatgtccgccgcgatcctgccagcctgaccaaaatgatgaccagttacgttatcggccaggcaatgaaagccggtaaatttaaagaaactgatttagtcactatcggcaacgacgcatgggccaccggtaacccggtgtttaaaggttcttcgctgatgttcctcaaaccgggcatgcaggttccggtttctcagctgatccgcggtattaacctgcaatcgggtaacgatgcttgtgtcgccatggccgattttgccgctggtagccaggacgcttttgttggcttgatgaacagctacgttaacgcactgggcctgaaaaatacccacttccagacggtacatggtctggatgctgatggtcagtacagctccgcgcgagatatggcgctgatcggccaggcattgatccgtgacgtaccgaatgaatactcgatctataaagaaaaagaatttacgtttaacggtattcgccagctgaaccgtaacggcctgttatgggataacagcctgaatgtcgacggcatcaaaaccggacacactgacaaagcaggttacaaccttgttgcttctgcgactgaaggccagatgcgcttgatttctgcggtaatgggcggacgtacttttaaaggccgtgaagccgaaagtaaaaaactgctaacctggggcttccgtttctttgaaaccgttaacccactgaaagtaggtaaagagttcgcctctgaaccggtttggtttggtgattctgatcgcgcttcgttaggggttgataaagacgtgtacctgaccattccgcgtggtcgcatgaaagatctgaaagccagctatgtgctgaacagcagtgaattgcatgcgccgctgcaaaagaatcaggtcgtcggaactatcaacttccagcttgatggcaaaacgatcgagcaacgcccgctggttgtgttgcaagaaatcccggaaggtaacttcttcggcaaaatcattgattacattaaattaatgttccatcactggtttggttaactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z