LOCUS       BBa_K1979004            2237 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP-PBP5
ACCESSION   BBa_K1979004
VERSION     BBa_K1979004.1  
FEATURES             Location/Qualifiers
     promoter        1..34
                     /label=J23101
     RBS             55..62
                     /label=g10-L rbs
     misc_feature    84..101
                     /label=his
     misc_feature    132..164
                     /label=T7
     CDS             174..890
                     /label=GFP
     CDS             891..2099
                     /label=PBP5
     misc_feature    2190..2237
                     /label=T7 terminator
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctacgaaata attttgttta actttaagaa
       61 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
      121 gcggcagcca tatggctagc atgactggtg gacagcaaat gggtcgcgga tccatggtga
      181 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      241 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      301 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      361 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      421 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      481 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      541 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      601 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      661 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      721 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      781 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      841 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aataccattt
      901 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
      961 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
     1021 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
     1081 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
     1141 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
     1201 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
     1261 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
     1321 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
     1381 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
     1441 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
     1501 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
     1561 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
     1621 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
     1681 tttctgcggt aatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
     1741 taacctgggg cttccgtttc tttgaaaccg ttaacccact gaaagtaggt aaagagttcg
     1801 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
     1861 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
     1921 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
     1981 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
     2041 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaac
     2101 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
     2161 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     2221 tcttgagggg ttttttg
//