BBa_K1979004 1 BBa_K1979004 GFP-PBP5 2016-08-27T11:00:00Z 2016-08-28T02:50:06Z The GFP and PBP5 sequences are retrieved from the GenBank, and the promoter comes from J23101. This device codes for the GFP-PBP5 fusion protein. false false _2446_ 29797 29797 9 false Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013). false MA Xinyi annotation2481800 1 his range2481800 1 84 101 annotation2482187 1 T7 terminator range2482187 1 2190 2237 annotation2481798 1 J23101 range2481798 1 1 34 annotation2482186 1 PBP5 range2482186 1 891 2099 annotation2482185 1 GFP range2482185 1 174 890 annotation2481801 1 T7 range2481801 1 132 164 annotation2481799 1 g10-L rbs range2481799 1 55 62 BBa_K1979004_sequence 1 tttacagctagctcagtcctaggtattatgctacgaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgactggtggacagcaaatgggtcgcggatccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaataccattttttccgctcgtatcatgaagcgcctggcgctcaccacggctctttgcacagcctttatctctgctgcacatgccgatgacctgaatatcaaaactatgatcccgggtgtaccgcagatcgatgcggagtcctacatcctgattgactataactccggcaaagtgctcgccgaacagaacgcagatgtccgccgcgatcctgccagcctgaccaaaatgatgaccagttacgttatcggccaggcaatgaaagccggtaaatttaaagaaactgatttagtcactatcggcaacgacgcatgggccaccggtaacccggtgtttaaaggttcttcgctgatgttcctcaaaccgggcatgcaggttccggtttctcagctgatccgcggtattaacctgcaatcgggtaacgatgcttgtgtcgccatggccgattttgccgctggtagccaggacgcttttgttggcttgatgaacagctacgttaacgcactgggcctgaaaaatacccacttccagacggtacatggtctggatgctgatggtcagtacagctccgcgcgagatatggcgctgatcggccaggcattgatccgtgacgtaccgaatgaatactcgatctataaagaaaaagaatttacgtttaacggtattcgccagctgaaccgtaacggcctgttatgggataacagcctgaatgtcgacggcatcaaaaccggacacactgacaaagcaggttacaaccttgttgcttctgcgactgaaggccagatgcgcttgatttctgcggtaatgggcggacgtacttttaaaggccgtgaagccgaaagtaaaaaactgctaacctggggcttccgtttctttgaaaccgttaacccactgaaagtaggtaaagagttcgcctctgaaccggtttggtttggtgattctgatcgcgcttcgttaggggttgataaagacgtgtacctgaccattccgcgtggtcgcatgaaagatctgaaagccagctatgtgctgaacagcagtgaattgcatgcgccgctgcaaaagaatcaggtcgtcggaactatcaacttccagcttgatggcaaaacgatcgagcaacgcccgctggttgtgttgcaagaaatcccggaaggtaacttcttcggcaaaatcattgattacattaaattaatgttccatcactggtttggttaactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z