BBa_K1979005
1
BBa_K1979005
CBD-PBP5
2016-09-16T11:00:00Z
2016-09-17T12:37:49Z
The PBP5 is from the E.coli's genes, CBD sequence is retrieved from GenBank, and the promoter is from part BBa_J23101.
This part codes for CBD (cellulose binding domain)- PBP5 protein.
false
false
_2446_
29797
29797
9
false
Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013).
false
MA Xinyi
annotation2483691
1
his
range2483691
1
84
101
annotation2483689
1
J23101
range2483689
1
1
35
annotation2483690
1
g10-L rbs
range2483690
1
58
64
annotation2483692
1
T7
range2483692
1
132
164
annotation2483694
1
PBP5
range2483694
1
321
1529
annotation2483695
1
T7 terminator
range2483695
1
1620
1667
annotation2483693
1
CBD
range2483693
1
174
320
BBa_K1979005_sequence
1
tttacagctagctcagtcctaggtattatgctacgaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgactggtggacagcaaatgggtcgcggatccggtcaggcttgctcttctgtttggggtcagtgcggtggtcagaactggtctggtccgacctgctgcgcttctggttctacctgcgtttactctaacgactactactctcagtgcctgccgggtgctgcttcttcttcttctggttctaataccattttttccgctcgtatcatgaagcgcctggcgctcaccacggctctttgcacagcctttatctctgctgcacatgccgatgacctgaatatcaaaactatgatcccgggtgtaccgcagatcgatgcggagtcctacatcctgattgactataactccggcaaagtgctcgccgaacagaacgcagatgtccgccgcgatcctgccagcctgaccaaaatgatgaccagttacgttatcggccaggcaatgaaagccggtaaatttaaagaaactgatttagtcactatcggcaacgacgcatgggccaccggtaacccggtgtttaaaggttcttcgctgatgttcctcaaaccgggcatgcaggttccggtttctcagctgatccgcggtattaacctgcaatcgggtaacgatgcttgtgtcgccatggccgattttgccgctggtagccaggacgcttttgttggcttgatgaacagctacgttaacgcactgggcctgaaaaatacccacttccagacggtacatggtctggatgctgatggtcagtacagctccgcgcgagatatggcgctgatcggccaggcattgatccgtgacgtaccgaatgaatactcgatctataaagaaaaagaatttacgtttaacggtattcgccagctgaaccgtaacggcctgttatgggataacagcctgaatgtcgacggcatcaaaaccggacacactgacaaagcaggttacaaccttgttgcttctgcgactgaaggccagatgcgcttgatttctgcggtaatgggcggacgtacttttaaaggccgtgaagccgaaagtaaaaaactgctaacctggggcttccgtttctttgaaaccgttaacccactgaaagtaggtaaagagttcgcctctgaaccggtttggtttggtgattctgatcgcgcttcgttaggggttgataaagacgtgtacctgaccattccgcgtggtcgcatgaaagatctgaaagccagctatgtgctgaacagcagtgaattgcatgcgccgctgcaaaagaatcaggtcgtcggaactatcaacttccagcttgatggcaaaacgatcgagcaacgcccgctggttgtgttgcaagaaatcccggaaggtaacttcttcggcaaaatcattgattacattaaattaatgttccatcactggtttggttaactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z