BBa_K1979005 1 BBa_K1979005 CBD-PBP5 2016-09-16T11:00:00Z 2016-09-17T12:37:49Z The PBP5 is from the E.coli's genes, CBD sequence is retrieved from GenBank, and the promoter is from part BBa_J23101. This part codes for CBD (cellulose binding domain)- PBP5 protein. false false _2446_ 29797 29797 9 false Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013). false MA Xinyi annotation2483691 1 his range2483691 1 84 101 annotation2483690 1 g10-L rbs range2483690 1 58 64 annotation2483694 1 PBP5 range2483694 1 321 1529 annotation2483693 1 CBD range2483693 1 174 320 annotation2483695 1 T7 terminator range2483695 1 1620 1667 annotation2483689 1 J23101 range2483689 1 1 35 annotation2483692 1 T7 range2483692 1 132 164 BBa_K1979005_sequence 1 tttacagctagctcagtcctaggtattatgctacgaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgactggtggacagcaaatgggtcgcggatccggtcaggcttgctcttctgtttggggtcagtgcggtggtcagaactggtctggtccgacctgctgcgcttctggttctacctgcgtttactctaacgactactactctcagtgcctgccgggtgctgcttcttcttcttctggttctaataccattttttccgctcgtatcatgaagcgcctggcgctcaccacggctctttgcacagcctttatctctgctgcacatgccgatgacctgaatatcaaaactatgatcccgggtgtaccgcagatcgatgcggagtcctacatcctgattgactataactccggcaaagtgctcgccgaacagaacgcagatgtccgccgcgatcctgccagcctgaccaaaatgatgaccagttacgttatcggccaggcaatgaaagccggtaaatttaaagaaactgatttagtcactatcggcaacgacgcatgggccaccggtaacccggtgtttaaaggttcttcgctgatgttcctcaaaccgggcatgcaggttccggtttctcagctgatccgcggtattaacctgcaatcgggtaacgatgcttgtgtcgccatggccgattttgccgctggtagccaggacgcttttgttggcttgatgaacagctacgttaacgcactgggcctgaaaaatacccacttccagacggtacatggtctggatgctgatggtcagtacagctccgcgcgagatatggcgctgatcggccaggcattgatccgtgacgtaccgaatgaatactcgatctataaagaaaaagaatttacgtttaacggtattcgccagctgaaccgtaacggcctgttatgggataacagcctgaatgtcgacggcatcaaaaccggacacactgacaaagcaggttacaaccttgttgcttctgcgactgaaggccagatgcgcttgatttctgcggtaatgggcggacgtacttttaaaggccgtgaagccgaaagtaaaaaactgctaacctggggcttccgtttctttgaaaccgttaacccactgaaagtaggtaaagagttcgcctctgaaccggtttggtttggtgattctgatcgcgcttcgttaggggttgataaagacgtgtacctgaccattccgcgtggtcgcatgaaagatctgaaagccagctatgtgctgaacagcagtgaattgcatgcgccgctgcaaaagaatcaggtcgtcggaactatcaacttccagcttgatggcaaaacgatcgagcaacgcccgctggttgtgttgcaagaaatcccggaaggtaacttcttcggcaaaatcattgattacattaaattaatgttccatcactggtttggttaactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z