BBa_K1980001 1 Csp1sfGFP TAT Copper Storage Protein 1 sfGFP 2016-10-10T11:00:00Z 2016-10-12T02:35:29Z The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT. Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker. false false _2447_ 29607 29607 9 false Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence. false Sam Garforth annotation2495230 1 sfGFP range2495230 1 469 1179 annotation2495238 1 Double stop range2495238 1 1198 1203 annotation2495229 1 linker range2495229 1 451 468 annotation2495227 1 TAT signal peptide range2495227 1 1 84 annotation2495231 1 Hexahistidine range2495231 1 1180 1197 annotation2495228 1 Csp1 range2495228 1 85 450 BBa_K1980001_sequence 1 atgcaacgtcgtgacttcctgaaatacagtgttgctcttggggttgcttccgcattacccctttggtcccgcgcagtgtttgctggtgaagacccgcacgcaggccataagatgtctcatggtgcgaagtacaaagcacttcttgactcttcctcccattgtgttgcggtaggcgaggactgtctgcgtcactgttttgaaatgcttgctatgaacgacgcgagtatgggggcgtgcaccaaagccacctacgatcttgtagccgcctgcggagcattagctaagctggcggggaccaactcagcctttacaccagccttcgccaaagtcgttgctgatgtatgtgccgcgtgcaaaaaggaatgtgacaagtttccatccattgccgaatgtaaggcttgcggagaggcgtgccaggcttgtgcagaagagtgtcacaaagtcgcggccggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z