BBa_K1980003 1 MymTsfGFP MymT sfGFP 2016-10-10T11:00:00Z 2016-10-20T07:26:41Z Mycobacterium tuberculosis. Ordered as codon optimised DNA. MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli. false false _2447_ 29607 29607 9 false This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. false Sam Garforth annotation2495248 1 Double stop range2495248 1 906 912 annotation2495245 1 sfGFP range2495245 1 178 888 annotation2495244 1 linker range2495244 1 160 177 annotation2495246 1 Hexahistidine range2495246 1 889 906 annotation2495243 1 MymT range2495243 1 1 159 BBa_K1980003_sequence 1 atgcgtgtaattcgcatgactaattatgaagccggtacacttttgacttgtagccacgaggggtgcggctgtcgtgttcgtattgaagtcccgtgtcattgtgccggagcgggcgacgcttaccgctgcacctgtggagatgaactggctcctgtgaaaggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z