LOCUS       BBa_K1980009            1554 bp    DNA     linear       26-Nov-2024
DEFINITION  pCusC CusR RFP
ACCESSION   BBa_K1980009
VERSION     BBa_K1980009.1  
FEATURES             Location/Qualifiers
     misc_feature    706..706
                     /label=t to c mutation (val to Ala)
     promoter        1..98
                     /label=pCusC
     RBS             105..116
                     /label=RBS
     misc_feature    60..65
                     /label=-35
     misc_feature    81..87
                     /label=-10
     CDS             123..809
                     /label=CusR
     RBS             822..827
                     /label=RBS
     CDS             838..1530
                     /label=mKate2
     misc_feature    1531..1554
                     /label=Hexahistidine
ORIGIN
        1 gcgggatcag atggcaatcg cttattggca aaatgacaat tttgtcattt ttctgtcacc
       61 ggaaaatcag agcctggcga gtaaagttgg cggcataata ctagaaagag gagaaattac
      121 agatgaagct gcttatcgtt gaggatgaaa aaaaaacagg cgaatatctt acaaaaggac
      181 tgacggaggc tggatttgta gttgacttag ccgacaatgg gcttaatggg taccatcttg
      241 caatgacggg ggactatgat ttaatcatct tagatatcat gttaccagac gtaaatggat
      301 gggatattgt gcgtatgttg cgctcagcta acaaaggaat gcccatcctg ctgctgaccg
      361 cgttaggaac tatcgaacat cgtgttaaag gactggaatt gggcgcagat gattatctgg
      421 taaaaccctt cgcttttgcc gaactgttag cacgtgtccg cactctgtta cgtcgcggcg
      481 ccgcggtcat cattgaatct caatttcagg tagcagacct tatggtagat cttgtaagtc
      541 gcaaggttac acgttctggt acacgcatca ccttaacctc caaggagttt acattgcttg
      601 agtttttcct tcgtcaccag ggagaagtcc ttccacgttc actgatcgca tctcaggttt
      661 gggacatgaa ttttgactca gacaccaacg caattgatgt ggcggccaag cgtttacgtg
      721 gaaagatcga caacgacttt gagcccaaac tgatccaaac ggtgcgtggg gttggatata
      781 tgctggaggt ccccgatggt caataataac atgctggtga gaggaggaat taacctcatg
      841 agcgaactaa tcaaagagaa catgcacatg aaattatata tggaaggtac tgtcaacaat
      901 catcatttca aatgcacatc cgaaggtgaa ggtaaaccat atgaaggcac acaaacaatg
      961 cgcatcaaag cagttgaagg tggacccctg ccctttgcgt ttgacattct cgcaacgagc
     1021 tttatgtacg ggtctaaaac ttttatcaat cacacccaag gcattcctga cttttttaaa
     1081 cagtcctttc ctgaaggctt tacctgggaa cgtgtaacaa cttatgaaga tggcggtgta
     1141 cttacagcaa ctcaagatac gagtttacaa gatggctgtc tgatttacaa tgttaaaatc
     1201 cgtggcgtaa atttcccgag taacggaccc gtaatgcaaa aaaaaactct tggttgggaa
     1261 gcatcaacag aaaccttata tcctgcggac ggtggcttag aaggacgcgc agacatggca
     1321 ctgaaattag ttggaggcgg tcatttaatc tgcaacctga aaacaaccta tcgttccaaa
     1381 aaacccgcta aaaaccttaa aatgcctgga gtatactatg ttgatcgtcg cttagaacgt
     1441 attaaagaag ctgataaaga aacctacgtt gaacaacatg aagtagccgt agcccgttat
     1501 tgtgaccttc cgtcgaaatt aggacatcgt catcatcacc accatcacta ataa
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