##gff-version 3
##sequence-region BBa_K1980009 1 1554
BBa_K1980009	.	CDS	123	809	.	+	0	ID=annotation2525375;Name=CusR
BBa_K1980009	.	CDS	838	1530	.	+	0	ID=annotation2525378;Name=mKate2
BBa_K1980009	.	ribosome_entry_site	822	827	.	+	0	ID=annotation2525377;Name=RBS
BBa_K1980009	.	sequence_feature	60	65	.	+	0	ID=annotation2525373;Name=-35
BBa_K1980009	.	ribosome_entry_site	105	116	.	+	0	ID=annotation2525372;Name=RBS
BBa_K1980009	.	promoter	1	98	.	+	0	ID=annotation2525371;Name=pCusC
BBa_K1980009	.	tag	1531	1554	.	+	0	ID=annotation2525379;Name=Hexahistidine
BBa_K1980009	.	sequence_feature	81	87	.	+	0	ID=annotation2525374;Name=-10
BBa_K1980009	.	sequence_alteration	706	706	.	+	0	ID=annotation2525368;Name=t to c mutation (val to Ala)
>BBa_K1980009
gcgggatcagatggcaatcgcttattggcaaaatgacaattttgtcatttttctgtcaccggaaaatcagagcctggcg
agtaaagttggcggcataatactagaaagaggagaaattacagatgaagctgcttatcgttgaggatgaaaaaaaaaca
ggcgaatatcttacaaaaggactgacggaggctggatttgtagttgacttagccgacaatgggcttaatgggtaccatc
ttgcaatgacgggggactatgatttaatcatcttagatatcatgttaccagacgtaaatggatgggatattgtgcgtat
gttgcgctcagctaacaaaggaatgcccatcctgctgctgaccgcgttaggaactatcgaacatcgtgttaaaggactg
gaattgggcgcagatgattatctggtaaaacccttcgcttttgccgaactgttagcacgtgtccgcactctgttacgtc
gcggcgccgcggtcatcattgaatctcaatttcaggtagcagaccttatggtagatcttgtaagtcgcaaggttacacg
ttctggtacacgcatcaccttaacctccaaggagtttacattgcttgagtttttccttcgtcaccagggagaagtcctt
ccacgttcactgatcgcatctcaggtttgggacatgaattttgactcagacaccaacgcaattgatgtggcggccaagc
gtttacgtggaaagatcgacaacgactttgagcccaaactgatccaaacggtgcgtggggttggatatatgctggaggt
ccccgatggtcaataataacatgctggtgagaggaggaattaacctcatgagcgaactaatcaaagagaacatgcacat
gaaattatatatggaaggtactgtcaacaatcatcatttcaaatgcacatccgaaggtgaaggtaaaccatatgaaggc
acacaaacaatgcgcatcaaagcagttgaaggtggacccctgccctttgcgtttgacattctcgcaacgagctttatgt
acgggtctaaaacttttatcaatcacacccaaggcattcctgacttttttaaacagtcctttcctgaaggctttacctg
ggaacgtgtaacaacttatgaagatggcggtgtacttacagcaactcaagatacgagtttacaagatggctgtctgatt
tacaatgttaaaatccgtggcgtaaatttcccgagtaacggacccgtaatgcaaaaaaaaactcttggttgggaagcat
caacagaaaccttatatcctgcggacggtggcttagaaggacgcgcagacatggcactgaaattagttggaggcggtca
tttaatctgcaacctgaaaacaacctatcgttccaaaaaacccgctaaaaaccttaaaatgcctggagtatactatgtt
gatcgtcgcttagaacgtattaaagaagctgataaagaaacctacgttgaacaacatgaagtagccgtagcccgttatt
gtgaccttccgtcgaaattaggacatcgtcatcatcaccaccatcactaataa