LOCUS       BBa_K1980010            1783 bp    DNA     linear       26-Dec-2024
DEFINITION  pCopA TAT Csp1 sfGFP with divergent CueR
ACCESSION   BBa_K1980010
VERSION     BBa_K1980010.1  
FEATURES             Location/Qualifiers
     misc_feature    563..574
                     /label=RBS
     misc_feature    1..7
                     /label=Double stop
     CDS             7..411
                     /label=CueR
     RBS             418..429
                     /label=RBS
     promoter        439..472
                     /label=Constitutive promoter
     promoter        506..556
                     /label=pCopA
     promoter        1..574
                     /label=BBa_K1980006
     misc_feature    581..664
                     /label=TAT  signal peptide
     CDS             665..1030
                     /label=Csp1
     misc_feature    1031..1048
                     /label=linker
     CDS             1049..1759
                     /label=sfGFP
     misc_feature    1760..1777
                     /label=Hexahistidine
     misc_feature    1778..1783
                     /label=Double stop
     CDS             581..1783
                     /label=BBa_K1980001
ORIGIN
        1 ttattaccct gcgcgatgat gacaacaacc cgacaggttt tcaataatcg gacagtcggc
       61 actatcgtcg cccggacacg cgttagccag cgccagcagt tggtcccgca tgctctgtaa
      121 ttcttcgata tggcgctcaa tttccgcaac tttttcaagg gtacggcgtt tcacatcagc
      181 agagtgacgt tgcggatcgt tgaacaaatt gaccagttca ccgctctcct caaggttgaa
      241 ccctacttga cgggcctggc gcagtaacgt caactcattg aggtgctgtt gcgtataggt
      301 gcgataacca ttctctgagc gcatcggcgg agtaaccaaa cctttttctt cataaaagcg
      361 gattgctttg ctggtcagac ctgtgatctt cgccacatcg gagatgttca tctgtaattt
      421 ctcctctttc tctagtagct agcacaatac ctaggactga gctagctgtc aaactagaga
      481 gtagatagat agatgcaccc cgcctggttt attaatttct tgaccttccc cttgctggaa
      541 ggtttaacct ttatcatact agaaagagga gaaatactag atgcaacgtc gtgacttcct
      601 gaaatacagt gttgctcttg gggttgcttc cgcattaccc ctttggtccc gcgcagtgtt
      661 tgctggtgaa gacccgcacg caggccataa gatgtctcat ggtgcgaagt acaaagcact
      721 tcttgactct tcctcccatt gtgttgcggt aggcgaggac tgtctgcgtc actgttttga
      781 aatgcttgct atgaacgacg cgagtatggg ggcgtgcacc aaagccacct acgatcttgt
      841 agccgcctgc ggagcattag ctaagctggc ggggaccaac tcagccttta caccagcctt
      901 cgccaaagtc gttgctgatg tatgtgccgc gtgcaaaaag gaatgtgaca agtttccatc
      961 cattgccgaa tgtaaggctt gcggagaggc gtgccaggct tgtgcagaag agtgtcacaa
     1021 agtcgcggcc ggcagtgggt ctgggtcgag caaaggagaa gaacttttca ctggagttgt
     1081 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tccgtggaga
     1141 gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca ctactggaaa
     1201 actacctgtt ccctggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
     1261 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1321 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
     1381 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aaaggtattg attttaaaga
     1441 agatggaaac attctcggac acaaactcga gtacaacttt aactcacaca atgtatacat
     1501 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
     1561 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     1621 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
     1681 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     1741 catggatgag ctctacaaac atcatcacca ccatcactaa taa
//