LOCUS       BBa_K1980012            1492 bp    DNA     linear       22-Dec-2024
DEFINITION  pCopA MymT sfGFP with divergent CueR
ACCESSION   BBa_K1980012
VERSION     BBa_K1980012.1  
FEATURES             Location/Qualifiers
     misc_feature    563..574
                     /label=RBS
     misc_feature    1..7
                     /label=Double stop
     CDS             7..411
                     /label=CueR
     RBS             418..429
                     /label=RBS
     promoter        439..472
                     /label=Constitutive promoter
     promoter        506..556
                     /label=pCopA
     promoter        1..574
                     /label=BBa_K1980006
     CDS             581..739
                     /label=MymT
     misc_feature    740..757
                     /label=linker
     CDS             758..1468
                     /label=sfGFP
     misc_feature    1469..1486
                     /label=Hexahistidine
     misc_feature    1486..1492
                     /label=Double stop
     CDS             581..1492
                     /label=BBa_K1980003
ORIGIN
        1 ttattaccct gcgcgatgat gacaacaacc cgacaggttt tcaataatcg gacagtcggc
       61 actatcgtcg cccggacacg cgttagccag cgccagcagt tggtcccgca tgctctgtaa
      121 ttcttcgata tggcgctcaa tttccgcaac tttttcaagg gtacggcgtt tcacatcagc
      181 agagtgacgt tgcggatcgt tgaacaaatt gaccagttca ccgctctcct caaggttgaa
      241 ccctacttga cgggcctggc gcagtaacgt caactcattg aggtgctgtt gcgtataggt
      301 gcgataacca ttctctgagc gcatcggcgg agtaaccaaa cctttttctt cataaaagcg
      361 gattgctttg ctggtcagac ctgtgatctt cgccacatcg gagatgttca tctgtaattt
      421 ctcctctttc tctagtagct agcacaatac ctaggactga gctagctgtc aaactagaga
      481 gtagatagat agatgcaccc cgcctggttt attaatttct tgaccttccc cttgctggaa
      541 ggtttaacct ttatcatact agaaagagga gaaatactag atgcgtgtaa ttcgcatgac
      601 taattatgaa gccggtacac ttttgacttg tagccacgag gggtgcggct gtcgtgttcg
      661 tattgaagtc ccgtgtcatt gtgccggagc gggcgacgct taccgctgca cctgtggaga
      721 tgaactggct cctgtgaaag gcagtgggtc tgggtcgagc aaaggagaag aacttttcac
      781 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      841 ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac
      901 tactggaaaa ctacctgttc cctggccaac acttgtcact actctgacct atggtgttca
      961 atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc
     1021 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg
     1081 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga
     1141 ttttaaagaa gatggaaaca ttctcggaca caaactcgag tacaacttta actcacacaa
     1201 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
     1261 caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg
     1321 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa
     1381 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
     1441 tacacatggc atggatgagc tctacaaaca tcatcaccac catcactaat aa
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