BBa_K1981001 1 BBa_K1981001 lsrA 2016-10-13T11:00:00Z 2016-10-14T06:23:15Z Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was chemically synthesized. Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. false false _2448_ 25366 25366 9 false Point mutation was performed to mutate the restriction site of XbaI at 192 bp. Condon optimization was performed to meet the manufacture requirement of IDT to synthesize the DNA fragment. false Yulei Dang, Xinhao Song annotation2501400 1 lsrA Coding Region range2501400 1 1 1200 annotation2501401 1 TGA Stop Codon range2501401 1 1198 1200 annotation2501398 1 ATG Start Codon range2501398 1 1 3 BBa_K1981001_sequence 1 atgacgatagaggaaaatatttgtatcggcctcaaggaaaaaaagaagaaattaaaagttcaaatacaacaacttatatcatctttgggttgggacatagcgttgaacaagttaggcggtacattaagcatagctcagcagcaattagttgagatagtgcgggggttgattcgtgaagcggagatactcatcttagatgagccgacttcaacattgacgactcatgagatcaaatccttgttcgtactcatgaagagtcttcaggagaaggggataggaatgatctatataacccatcgtttcccagaaatctttgaaatcgctaacaaggttgctatattacgcgatgggatggtggtcagtcagggtgacgtctgcgattacacgtacgatatgctcatggagggccttttacctaagggttataaacagaaggaaaaaatagaagtcgtgcaagagacagctaaaacgaagaaaattttggaggtcgttgacattaacgggcacgcgttcgaaaacataagttttaccgtacatgcaggcgaggttgtaggcgtggcagggatcatcgggagtggaagaacagagctcgccgaggccatctttggcctcaaggcaatcaaaagcggctctattttactggaaggaaagtccatagatacatgttctctgcacaagagattgtccgaaggtttggtttatgtgccagaagatagagccagaaacggtattttttctatcgcgtcagtaaaagaaaatatagctgctgctactagcttgtatcagaataatcgtttctttattaatcaagagaaagagtcagcactggttaattcatttatcgagcaattcagaattgtggttcagaacatgaacaaggaattggcatctttgtctggaggcaatcagcaaaaagtagtgctggccaaatacctggcctgcaatcctaaaatcataatcctggatgagcctaccagaggaattgatgccaaggctcggttggaggtctacgaaactattaagaagatgaaacgcgaagggttagcaattttgctgatctcatcagatgtcgaagaaatagtccaattggtaaacagagtttacgtcatgcgtaatggtcgtttcgtctcccacatggaaaaagagcaactctcagtgaacgaggtgactagacttgcctatggcggagtagcggaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z