BBa_K1981004 1 BBa_K1981004 lsrD 2016-10-13T11:00:00Z 2016-10-14T06:21:19Z Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was chemically synthesized. Autoinducer 2 import system permease protein LsrD. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Probably responsible for the translocation of the substrate across the membrane. Share the same promoter with lsrA. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. false false _2448_ 25366 25366 9 false Point mutation was performed to mutate the restriction site of EcoRI at 52 bp and SpeI 345 bp. Condon optimization was performed to meet the manufacture requirement of IDT to synthesize the DNA fragment. false Yulei Dang, Xinhao Song annotation2501991 1 lsrD Coding Region range2501991 1 1 990 annotation2501992 1 TGA Stop Codon range2501992 1 988 990 annotation2501990 1 ATG Start Codon range2501990 1 1 3 BBa_K1981004_sequence 1 atgaagtatttatatagatgggaaggggttctaattgtattacttcttatagagttcatcctttttagttttataaatagtgatttcttaaatattagtaatctcctttttagtacaaatgatttcctatttatcgcaattgcggcgataccaatgactttcgttattgtaacaggaggaattgatgtatcggtcggatcaataatggggctcacttcgatcttgattggtgtactttggatgaacggaatacctattttgtttgctgtcataatcgccctaataattagttgtttagcaggagctttaaatggactcattataaaaatgacagatgtagaaccacttgtcgttacactcggaacgatgtttttatacggaggaattgcgcttgtcatttcaggaggggcgggtgcttctggatatgaagggataagcggtttacctgatacttatgtacaactagcaaatggtagttttataggaatacccaatttattatggttacttatttcattaacaattgtatacacaattttgtttcaccgaacgatatatggacgctacgtaaaattaacaggtgcgaatgaaaatacagcaaaatacaccgggattaagacgaagaaagtagtaatcattgcttacatactttctgggttaggaggtggattagggggagttttcttaactgcatatttcggttctgcacgtgctgatataggaagtgaaacaatattaccaatcattacagcagtcgtattaggcgggacacttattacaggtggaaaaggaagtattttcggtactgtacttgcaagtatttttataggccttatgcaatatggcctacaaatgacaggcttaacaaatgaacaatcaaatgtcgtaataggtattatccttattctttcagttattatgagacatttcaaattacatcaatttttagcggggaaaagacagagttggcataagggggaaatgtcatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z