BBa_K1982000 1 TC tCas9-CIBN (Prokaryotic LACE system) 2016-09-09T11:00:00Z 2016-10-18T10:12:41Z GE Share tCas9 is a standardized (RFC 10) protein.Interacting with a DNA-binding RNA and fused with different effector domains it can be used for specific gene regulation. Cas9 is the main protein of the CRISPR/Cas system II of Streptococcus pyogenes. CRISPR systems protect bacteria and archaea from phages by recognizing and cleaving of invading phage DNA.This recognition is based on Watson Crick base pairing between a short RNA, called crRNA, and the complementary DNA strand. A second RNA, called tracrRNA, connects crRNA and Cas9. These three parts together form a protein-RNA-DNA complex with the targeted DNA strand [1] Cas9 became of great interest for research concerning DNA targeting, because of its ability to recognize site specific DNA strands by a crRNA. At first the functionality of Cas9 was modified by exchanging aminoacids. As a result, Cas9 was able to introduce mutations within the genome of several organisms by causing double strand breaks [2][3]. Then, it was converted from a nuclease to a nickase introducing single strand breaks [4] and lately it was converted to an enzymatically inactive form, called dCas9 [5]. This tCas9 is standardized (RFC 10). It can be used as a DNA binding protein, that can be fused with different effectors in order to regulate gene expression. false false _2449_ 30176 30176 9 false mutant Pst 1 :gctacctgcaG to gctacctgcaC mutant Pst 1 :actatctgcaG to actatctgcaC false Zexu Li annotation2517184 1 Prokaryotic rbs range2517184 1 1 18 annotation2517189 1 tCas9 range2517189 1 22 4122 annotation2517186 1 HA range2517186 1 4669 4725 annotation2517185 1 ATG range2517185 1 19 21 annotation2517187 1 TAATAA range2517187 1 4726 4731 annotation2517188 1 CIBN range2517188 1 4123 4668 BBa_K1982000_sequence 1 aaagaggagaaatactagatggacaagaagtactccattggcctcgccatcggaacaaatagcgtgggctgggctgtcatcacagatgagtacaaggtgcctagcaagaaatttaaggtgctgggaaatacagacagacatagcatcaagaagaacctcattggcgctctcctgtttgactccggcgaaacagccgaagctaccagactcaagagaaccgctaggagaaggtacaccagaaggaaaaacaggatttgctacctgcacgaaattttttccaacgagatggccaaggtggacgattccttcttccataggctggaagagagcttcctcgtggaggaagacaagaaacacgagaggcatcctatttttggcaatattgtggatgaggtcgcctaccatgagaagtatcccacaatctatcatctgagaaaaaaactggtggatagcaccgacaaggccgatctcaggctcatttatctcgctctggctcacatgatcaagtttaggggccacttcctgatcgaaggcgacctgaatcccgacaactccgacgtggacaaactgttcatccagctcgtccagacctacaatcaactcttcgaggagaaccccatcaatgcttccggcgtggatgccaaggccatcctgagcgctaggctctccaagtccaggaggctggaaaatctgatcgcccaactccctggagagaagaagaacggcctgtttggcaatctgattgccctgagcctcggactcacccccaacttcaagagcaacttcgatctcgccgaagacgccaaactccaactgagcaaggatacctacgacgacgatctcgataatctcctcgcccagatcggcgatcaatatgccgacctctttctggccgccaaaaacctgagcgacgctattctgctcagcgacattctcagggtgaatacagaaatcacaaaagcccccctgtccgccagcatgatcaaaaggtacgatgaacaccatcaggacctcaccctgctgaaggctctggtcaggcagcaactccccgaaaagtacaaggagattttctttgatcagtccaagaatggatatgctggctatattgatggaggcgcctcccaggaggaattttataaattcatcaagcccattctcgaaaagatggacggaaccgaagagctgctggtcaaactcaatagggaggatctgctgaggaagcaaaggaccttcgacaatggcagcatcccccaccagatccacctcggcgaactccacgctatcctcaggaggcaggaagacttctaccctttcctgaaggataacagggagaaaatcgagaaaatcctgaccttcagaatcccctactacgtcggacctctcgccaggggcaattccagattcgcctggatgacaaggaagagcgaggaaacaatcacaccatggaacttcgaagaagtggtcgataagggcgccagcgcccagagcttcattgaaaggatgaccaactttgataagaacctgcccaatgagaaggtgctgcctaagcactccctgctgtatgagtatttcaccgtgtataatgagctgaccaaggtcaagtacgtcaccgagggaatgagaaagcctgcttttctctccggcgagcagaaaaaagccatcgtggacctgctgttcaagaccaacaggaaggtgaccgtcaagcaactcaaggaggactactttaagaagattgagtgctttgatagcgtggaaattagcggagtcgaggacaggttcaatgcctccctcggaacatatcacgacctgctgaaaatcatcaaagacaaagattttctggataacgaggagaatgaagacattctggaggacattgtcctcaccctgaccctgtttgaggacagagagatgattgaagagaggctgaaaacctatgcccacctgttcgacgacaaggtgatgaagcagctcaaaagaaggagatataccggctggggcagactgtccaggaagctgatcaacggcattagggacaagcagagcggcaagaccattctcgactttctcaagtccgacggattcgccaacagaaactttatgcaactgatccacgatgacagcctcacctttaaggaagatattcagaaggctcaggtcagcggccaaggcgattccctccatgagcacatcgctaatctggctggctcccctgctatcaaaaagggcatcctccagacagtcaaagtcgtcgatgagctggtcaaggtgatgggcaggcataaacccgagaacattgtgattgagatggctagggagaaccagaccacccagaaaggccagaaaaacagcagagaaagaatgaagaggatcgaggagggcatcaaagaactgggcagccaaatcctcaaggagcaccccgtcgaaaatacacaactccagaacgaaaaactctacctctactatctgcacaacggcagagacatgtacgtggaccaggaactggacatcaacaggctctccgattacgatgtggacgccatcgtccctcagtcctttctgaaagatgatagcatcgacaacaaggtgctgaccaggtccgacaagaataggggcaagagcgataatgtgccctccgaggaggtcgtcaaaaaaatgaaaaactactggagacaactcctcaacgctaagctcatcacccaaagaaagttcgacaatctgaccaaagccgagaggggcggcctctccgaactggacaaggccggcttcatcaaaaggcaattggtggaaaccaggcagattacaaagcatgtcgctcaaattctcgatagcaggatgaataccaaatatgacgagaacgacaagctgatcagagaggtcaaggtcatcacactcaagtccaagctcgtgagcgacttcagaaaagatttccaattttataaagtcagggagatcaacaattaccaccacgctcacgacgcttatctcaacgctgtcgtgggaaccgccctgatcaaaaaataccccaagctggaaagcgagttcgtgtatggcgattataaagtgtacgacgtgaggaagatgatcgctaaaagcgagcaggaaatcggcaaggctacagccaagtactttttctactccaacattatgaacttcttcaagaccgagattaccctcgccaacggcgaaattaggaagaggcccctgattgaaacaaatggagaaacaggcgaaatcgtctgggacaagggcagggacttcgccacagtcagaaaagtgctgtccatgcctcaagtcaacatcgtcaaaaagaccgaggtgcagaccggcggctttagcaaagaaagcatcctgcccaagagaaactccgacaagctcatcgctaggaagaaggactgggaccctaagaaatacggaggatttgactcccctaccgtcgcctattccgtcctcgtcgtcgctaaggtggagaagggcaagagcaagaagctcaagagcgtcaaggagctgctgggaatcaccatcatggagaggagctccttcgaaaaaaaccctattgatttcctggaggccaagggctacaaggaggtcaagaaggacctcatcatcaagctgcccaaatacagcctcttcgaactggaaaatggcaggaagagaatgctcgctagggccggcgagctccagaaaggaaatgagctggctctgcccagcaagtacgtcaacttcctctatctcgccagccactatgaaaagctcaagggcagccccgaagacaatgagcagaagcagctcttcgtcgagcagcacaagcactacctcgatgaaatcatcgagcaaatcagcgagttttccaaaagggtgattctcgccgacgctaacctcgataaggtcctctccgcttacaacaagcatagagacaagcccatcagagaacaggccgagaacatcatccacctgtttacactcacaaacctcggagcccctgccgcttttaaatacttcgatacaaccattgataggaagaggtacacctccaccaaggaggtgctggatgctaccctgattcatcaatccatcacaggactctacgaaacaaggattgacctgtcccaactgggaggcgacaatggagctataggaggtgaccttttgctcaattttcctgacatgtcggtcctagagcgccaaagggctcacctcaagtacctcaatcccacctttgattctcctctcgccggcttctttgccgattcttcaatgattaccggcggcgagatggacagctatctttcgactgccggtttgaatcttccgatgatgtacggtgagacgacggtggaaggtgattcaagactctcaatttcgccggaaacgacgcttgggactggaaatttcaagaaacggaagtttgatacagagactaaggattgtaatgagaagaagaagaagatgacgatgaacagagatgacctagtagaagaaggagaagaagagaagtcgaaaataacagagcaaaacaatgggagcacaaaaagcatcaagaagatgaaacacaaagccaagaaagaagagaacaatttctctaatgattcatctaaagtgacgaaggaattggagaaaacggattatattcatgttcgtgcacgacgaggccaagccactgatagtcacagcatagcagaacgaaagagaaaagatcagtacccatacgatgttccagattacgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z