BBa_K1982004 1 BBa_K1982004 tCas9-CIBN (Prokaryotic LACE system) 2016-09-11T11:00:00Z 2016-10-18T10:12:30Z CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1]. we fused the light-inducible heterodimerizering proteins CRY2 and CIB1 from Arabidopsis thaliana to the VP64 transactivation domain and C-terminus of tCas9. false false _2449_ 30176 30176 9 false The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a tCas9 fused to its N terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription. false Zexu Li annotation2483005 1 ATG range2483005 1 4105 4107 annotation2483006 1 TAATAA range2483006 1 4726 4731 annotation2483003 1 ATG range2483003 1 19 21 annotation2517191 1 Prokaryotic rbs range2517191 1 1 18 annotation2483007 1 CIBN range2483007 1 4123 4668 annotation2517190 1 HA range2517190 1 4669 4725 annotation2483004 1 tCas9 range2483004 1 22 4122 BBa_K1982004_sequence 1 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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z