LOCUS       BBa_K1982005            2100 bp    DNA     linear       06-May-2024
DEFINITION  CRY2-VP64(Prokaryotic LACE system)
ACCESSION   BBa_K1982005
VERSION     BBa_K1982005.1  
FEATURES             Location/Qualifiers
     RBS             1..18
                     /label=Prokaryotic rbs
     misc_feature    19..21
                     /label=ATG
     CDS             22..1854
                     /label=CRY2
     misc_feature    1855..1875
                     /label=SV40 NLS
     misc_feature    1876..1914
                     /label=VP16
     misc_feature    1915..1953
                     /label=VP16
     misc_feature    1954..1992
                     /label=VP16
     misc_feature    1993..2031
                     /label=VP16
     misc_feature    2032..2037
                     /label=Linker
     misc_feature    2037..2063
                     /label=HA
     misc_feature    2071..2094
                     /label=FLAG
     misc_feature    2095..2100
                     /label=TAATAA
ORIGIN
        1 aaagaggaga aatactagat gaagatggac aaaaagacta tagtttggtt tagaagagac
       61 ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc tgtttttcct
      121 gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc ttcaagatgg
      181 tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg atctgacctc
      241 actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg cgttaccggt
      301 gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg ggaccatacc
      361 gtaaaggaga agctggtgga acgtgggatc tctgtgcaaa gctacaatgg agatctattg
      421 tatgaaccgt gggagatata ctgcgaaaag ggcaaacctt ttacgagttt caattcttac
      481 tggaagaaat gcttagatat gtcgattgaa tccgttatgc ttcctcctcc ttggcggttg
      541 atgccaataa ctgctgcggc tgaagcgatt tgggcgtgtt cgattgaaga actagggctg
      601 gagaatgagg ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc tccaggatgg
      661 agcaatgctg ataagttact aaatgagttc atcgagaagc agttgataga ttatgcaaag
      721 aacagcaaga aagttgttgg gaattgtact tcactacttt ctccgtatct ccatttcggg
      781 gaaataagcg tcagacacgt tttccagtgt gcccggatga aacaaattat atgggcaaga
      841 gataagaaca gtgaaggaga agaaagtgca gatctttttc ttaggggaat cggtttaaga
      901 gagtattctc ggtatatatg tttcaacttc ccgtttactc acgagcaatc gttgttgagt
      961 catcttcggt ttttcccttg ggatgctgat gttgataagt tcaaggcctg gagacaaggc
     1021 aggaccggtt atccgttggt ggatgccgga atgagagagc tttgggctac cggatggatg
     1081 cataacagaa taagagtgat tgtttcaagc tttgctgtga agtttcttct ccttccatgg
     1141 aaatggggaa tgaagtattt ctgggataca cttttggatg ctgatttgga atgtgacatc
     1201 cttggctggc agtatatctc tgggagtatc cccgatggcc acgagcttga tcgcttggac
     1261 aatcccgcgt tacaaggcgc caaatatgac ccagaaggtg agtacataag gcaatggctt
     1321 cccgagcttg cgagattgcc aactgaatgg atccatcatc catgggacgc tcctttaacc
     1381 gtactcaaag cttctggtgt ggaactcgga acaaactatg cgaaacccat tgtagacatc
     1441 gacacagctc gtgagctact agctaaagct atttcaagaa cccgtgaagc acagatcatg
     1501 atcggagcag cacctgatga gattgtagca gatagcttcg aggccttagg ggctaatacc
     1561 attaaagaac ctggtctttg cccatctgtg tcttctaatg accaacaagt accttcggct
     1621 gttcgttaca acgggtcaaa gagagtgaaa cctgaggaag aagaagagag agacatgaag
     1681 aaatctaggg gattcgatga aagggagttg ttttcgactg ctgaatcttc ttcttcttcg
     1741 agtgtgtttt tcgtttcgca gtcttgctcg ttggcatcag aagggaagaa tctggaaggt
     1801 attcaagatt catctgatca gattactaca agtttgggaa aaaatggttg caaaccgaaa
     1861 aagaaacgca aagttgggcg cgccgacgcg ctggacgatt tcgatctcga catgctgggt
     1921 tctgatgccc tcgatgactt tgacctggat atgttgggaa gcgacgcatt ggatgacttt
     1981 gatctggaca tgctcggctc cgatgctctg gacgatttcg atctcgatat gttaattaac
     2041 tacccgtacg acgttccgga ctacgcttct gactacaagg acgacgacga caaataataa
//