##gff-version 3
##sequence-region BBa_K1982006 1 4368
BBa_K1982006	.	sequence_feature	4222	4260	.	+	0	ID=annotation2517247;Name=VP16
BBa_K1982006	.	sequence_feature	4183	4221	.	+	0	ID=annotation2517246;Name=VP16
BBa_K1982006	.	ribosome_entry_site	1	18	.	+	0	ID=annotation2517241;Name=Prokaryotic rbs
BBa_K1982006	.	tag	4306	4332	.	+	0	ID=annotation2517250;Name=HA
BBa_K1982006	.	sequence_feature	4123	4143	.	+	0	ID=annotation2517244;Name=SV40 NLS
BBa_K1982006	.	stop_codon	4363	4368	.	+	0	ID=annotation2517252;Name=TAATAA
BBa_K1982006	.	CDS	22	4122	.	+	0	ID=annotation2517243;Name=tCas9
BBa_K1982006	.	start_codon	19	21	.	+	0	ID=annotation2517242;Name=ATG
BBa_K1982006	.	sequence_feature	4300	4305	.	+	0	ID=annotation2517249;Name=Linker
BBa_K1982006	.	sequence_feature	4261	4299	.	+	0	ID=annotation2517248;Name=VP16
BBa_K1982006	.	tag	4339	4362	.	+	0	ID=annotation2517251;Name=FLAG
BBa_K1982006	.	sequence_feature	4144	4182	.	+	0	ID=annotation2517245;Name=VP16
>BBa_K1982006
aaagaggagaaatactagatggacaagaagtactccattggcctcgccatcggaacaaatagcgtgggctgggctgtca
tcacagatgagtacaaggtgcctagcaagaaatttaaggtgctgggaaatacagacagacatagcatcaagaagaacct
cattggcgctctcctgtttgactccggcgaaacagccgaagctaccagactcaagagaaccgctaggagaaggtacacc
agaaggaaaaacaggatttgctacctgcacgaaattttttccaacgagatggccaaggtggacgattccttcttccata
ggctggaagagagcttcctcgtggaggaagacaagaaacacgagaggcatcctatttttggcaatattgtggatgaggt
cgcctaccatgagaagtatcccacaatctatcatctgagaaaaaaactggtggatagcaccgacaaggccgatctcagg
ctcatttatctcgctctggctcacatgatcaagtttaggggccacttcctgatcgaaggcgacctgaatcccgacaact
ccgacgtggacaaactgttcatccagctcgtccagacctacaatcaactcttcgaggagaaccccatcaatgcttccgg
cgtggatgccaaggccatcctgagcgctaggctctccaagtccaggaggctggaaaatctgatcgcccaactccctgga
gagaagaagaacggcctgtttggcaatctgattgccctgagcctcggactcacccccaacttcaagagcaacttcgatc
tcgccgaagacgccaaactccaactgagcaaggatacctacgacgacgatctcgataatctcctcgcccagatcggcga
tcaatatgccgacctctttctggccgccaaaaacctgagcgacgctattctgctcagcgacattctcagggtgaataca
gaaatcacaaaagcccccctgtccgccagcatgatcaaaaggtacgatgaacaccatcaggacctcaccctgctgaagg
ctctggtcaggcagcaactccccgaaaagtacaaggagattttctttgatcagtccaagaatggatatgctggctatat
tgatggaggcgcctcccaggaggaattttataaattcatcaagcccattctcgaaaagatggacggaaccgaagagctg
ctggtcaaactcaatagggaggatctgctgaggaagcaaaggaccttcgacaatggcagcatcccccaccagatccacc
tcggcgaactccacgctatcctcaggaggcaggaagacttctaccctttcctgaaggataacagggagaaaatcgagaa
aatcctgaccttcagaatcccctactacgtcggacctctcgccaggggcaattccagattcgcctggatgacaaggaag
agcgaggaaacaatcacaccatggaacttcgaagaagtggtcgataagggcgccagcgcccagagcttcattgaaagga
tgaccaactttgataagaacctgcccaatgagaaggtgctgcctaagcactccctgctgtatgagtatttcaccgtgta
taatgagctgaccaaggtcaagtacgtcaccgagggaatgagaaagcctgcttttctctccggcgagcagaaaaaagcc
atcgtggacctgctgttcaagaccaacaggaaggtgaccgtcaagcaactcaaggaggactactttaagaagattgagt
gctttgatagcgtggaaattagcggagtcgaggacaggttcaatgcctccctcggaacatatcacgacctgctgaaaat
catcaaagacaaagattttctggataacgaggagaatgaagacattctggaggacattgtcctcaccctgaccctgttt
gaggacagagagatgattgaagagaggctgaaaacctatgcccacctgttcgacgacaaggtgatgaagcagctcaaaa
gaaggagatataccggctggggcagactgtccaggaagctgatcaacggcattagggacaagcagagcggcaagaccat
tctcgactttctcaagtccgacggattcgccaacagaaactttatgcaactgatccacgatgacagcctcacctttaag
gaagatattcagaaggctcaggtcagcggccaaggcgattccctccatgagcacatcgctaatctggctggctcccctg
ctatcaaaaagggcatcctccagacagtcaaagtcgtcgatgagctggtcaaggtgatgggcaggcataaacccgagaa
cattgtgattgagatggctagggagaaccagaccacccagaaaggccagaaaaacagcagagaaagaatgaagaggatc
gaggagggcatcaaagaactgggcagccaaatcctcaaggagcaccccgtcgaaaatacacaactccagaacgaaaaac
tctacctctactatctgcacaacggcagagacatgtacgtggaccaggaactggacatcaacaggctctccgattacga
tgtggacgccatcgtccctcagtcctttctgaaagatgatagcatcgacaacaaggtgctgaccaggtccgacaagaat
aggggcaagagcgataatgtgccctccgaggaggtcgtcaaaaaaatgaaaaactactggagacaactcctcaacgcta
agctcatcacccaaagaaagttcgacaatctgaccaaagccgagaggggcggcctctccgaactggacaaggccggctt
catcaaaaggcaattggtggaaaccaggcagattacaaagcatgtcgctcaaattctcgatagcaggatgaataccaaa
tatgacgagaacgacaagctgatcagagaggtcaaggtcatcacactcaagtccaagctcgtgagcgacttcagaaaag
atttccaattttataaagtcagggagatcaacaattaccaccacgctcacgacgcttatctcaacgctgtcgtgggaac
cgccctgatcaaaaaataccccaagctggaaagcgagttcgtgtatggcgattataaagtgtacgacgtgaggaagatg
atcgctaaaagcgagcaggaaatcggcaaggctacagccaagtactttttctactccaacattatgaacttcttcaaga
ccgagattaccctcgccaacggcgaaattaggaagaggcccctgattgaaacaaatggagaaacaggcgaaatcgtctg
ggacaagggcagggacttcgccacagtcagaaaagtgctgtccatgcctcaagtcaacatcgtcaaaaagaccgaggtg
cagaccggcggctttagcaaagaaagcatcctgcccaagagaaactccgacaagctcatcgctaggaagaaggactggg
accctaagaaatacggaggatttgactcccctaccgtcgcctattccgtcctcgtcgtcgctaaggtggagaagggcaa
gagcaagaagctcaagagcgtcaaggagctgctgggaatcaccatcatggagaggagctccttcgaaaaaaaccctatt
gatttcctggaggccaagggctacaaggaggtcaagaaggacctcatcatcaagctgcccaaatacagcctcttcgaac
tggaaaatggcaggaagagaatgctcgctagggccggcgagctccagaaaggaaatgagctggctctgcccagcaagta
cgtcaacttcctctatctcgccagccactatgaaaagctcaagggcagccccgaagacaatgagcagaagcagctcttc
gtcgagcagcacaagcactacctcgatgaaatcatcgagcaaatcagcgagttttccaaaagggtgattctcgccgacg
ctaacctcgataaggtcctctccgcttacaacaagcatagagacaagcccatcagagaacaggccgagaacatcatcca
cctgtttacactcacaaacctcggagcccctgccgcttttaaatacttcgatacaaccattgataggaagaggtacacc
tccaccaaggaggtgctggatgctaccctgattcatcaatccatcacaggactctacgaaacaaggattgacctgtccc
aactgggaggcgacccgaaaaagaaacgcaaagttgggcgcgccgacgcgctggacgatttcgatctcgacatgctggg
ttctgatgccctcgatgactttgacctggatatgttgggaagcgacgcattggatgactttgatctggacatgctcggc
tccgatgctctggacgatttcgatctcgatatgttaattaactacccgtacgacgttccggactacgcttctgactaca
aggacgacgacgacaaataataa