LOCUS BBa_K1982011 4368 bp DNA linear 14-Nov-2024 DEFINITION tCas9-Vp64(Eukaryoticc) ACCESSION BBa_K1982011 VERSION BBa_K1982011.1 FEATURES Location/Qualifiers RBS 1..18 /label=Eukaryotic rbs misc_feature 19..21 /label=ATG CDS 22..4122 /label=tCas9 misc_feature 4123..4143 /label=SV40 NLS misc_feature 4144..4182 /label=VP16 misc_feature 4183..4221 /label=VP16 misc_feature 4222..4260 /label=VP16 misc_feature 4261..4299 /label=VP16 misc_feature 4300..4305 /label=Linker misc_feature 4306..4332 /label=HA misc_feature 4339..4362 /label=FLAG misc_feature 4363..4368 /label=TAATAA ORIGIN 1 cccgccgcca ccatggagat ggacaagaag tactccattg gcctcgccat cggaacaaat 61 agcgtgggct gggctgtcat cacagatgag tacaaggtgc ctagcaagaa atttaaggtg 121 ctgggaaata cagacagaca tagcatcaag aagaacctca ttggcgctct cctgtttgac 181 tccggcgaaa cagccgaagc taccagactc aagagaaccg ctaggagaag gtacaccaga 241 aggaaaaaca ggatttgcta cctgcacgaa attttttcca acgagatggc caaggtggac 301 gattccttct tccataggct ggaagagagc ttcctcgtgg aggaagacaa gaaacacgag 361 aggcatccta tttttggcaa tattgtggat gaggtcgcct accatgagaa gtatcccaca 421 atctatcatc tgagaaaaaa actggtggat agcaccgaca aggccgatct caggctcatt 481 tatctcgctc tggctcacat gatcaagttt aggggccact tcctgatcga aggcgacctg 541 aatcccgaca actccgacgt ggacaaactg ttcatccagc tcgtccagac ctacaatcaa 601 ctcttcgagg agaaccccat caatgcttcc ggcgtggatg ccaaggccat cctgagcgct 661 aggctctcca agtccaggag gctggaaaat ctgatcgccc aactccctgg agagaagaag 721 aacggcctgt ttggcaatct gattgccctg agcctcggac tcacccccaa cttcaagagc 781 aacttcgatc tcgccgaaga cgccaaactc caactgagca aggataccta cgacgacgat 841 ctcgataatc tcctcgccca gatcggcgat caatatgccg acctctttct ggccgccaaa 901 aacctgagcg acgctattct gctcagcgac attctcaggg tgaatacaga aatcacaaaa 961 gcccccctgt ccgccagcat gatcaaaagg tacgatgaac accatcagga cctcaccctg 1021 ctgaaggctc tggtcaggca gcaactcccc gaaaagtaca aggagatttt ctttgatcag 1081 tccaagaatg gatatgctgg ctatattgat ggaggcgcct cccaggagga attttataaa 1141 ttcatcaagc ccattctcga aaagatggac ggaaccgaag agctgctggt caaactcaat 1201 agggaggatc tgctgaggaa gcaaaggacc ttcgacaatg gcagcatccc ccaccagatc 1261 cacctcggcg aactccacgc tatcctcagg aggcaggaag acttctaccc tttcctgaag 1321 gataacaggg agaaaatcga gaaaatcctg accttcagaa tcccctacta cgtcggacct 1381 ctcgccaggg gcaattccag attcgcctgg atgacaagga agagcgagga aacaatcaca 1441 ccatggaact tcgaagaagt ggtcgataag ggcgccagcg cccagagctt cattgaaagg 1501 atgaccaact ttgataagaa cctgcccaat gagaaggtgc tgcctaagca ctccctgctg 1561 tatgagtatt tcaccgtgta taatgagctg accaaggtca agtacgtcac cgagggaatg 1621 agaaagcctg cttttctctc cggcgagcag aaaaaagcca tcgtggacct gctgttcaag 1681 accaacagga aggtgaccgt caagcaactc aaggaggact actttaagaa gattgagtgc 1741 tttgatagcg tggaaattag cggagtcgag gacaggttca atgcctccct cggaacatat 1801 cacgacctgc tgaaaatcat caaagacaaa gattttctgg ataacgagga gaatgaagac 1861 attctggagg acattgtcct caccctgacc ctgtttgagg acagagagat gattgaagag 1921 aggctgaaaa cctatgccca cctgttcgac gacaaggtga tgaagcagct caaaagaagg 1981 agatataccg gctggggcag actgtccagg aagctgatca acggcattag ggacaagcag 2041 agcggcaaga ccattctcga ctttctcaag tccgacggat tcgccaacag aaactttatg 2101 caactgatcc acgatgacag cctcaccttt aaggaagata ttcagaaggc tcaggtcagc 2161 ggccaaggcg attccctcca tgagcacatc gctaatctgg ctggctcccc tgctatcaaa 2221 aagggcatcc tccagacagt caaagtcgtc gatgagctgg tcaaggtgat gggcaggcat 2281 aaacccgaga acattgtgat tgagatggct agggagaacc agaccaccca gaaaggccag 2341 aaaaacagca gagaaagaat gaagaggatc gaggagggca tcaaagaact gggcagccaa 2401 atcctcaagg agcaccccgt cgaaaataca caactccaga acgaaaaact ctacctctac 2461 tatctgcaca acggcagaga catgtacgtg gaccaggaac tggacatcaa caggctctcc 2521 gattacgatg tggacgccat cgtccctcag tcctttctga aagatgatag catcgacaac 2581 aaggtgctga ccaggtccga caagaatagg ggcaagagcg ataatgtgcc ctccgaggag 2641 gtcgtcaaaa aaatgaaaaa ctactggaga caactcctca acgctaagct catcacccaa 2701 agaaagttcg acaatctgac caaagccgag aggggcggcc tctccgaact ggacaaggcc 2761 ggcttcatca aaaggcaatt ggtggaaacc aggcagatta caaagcatgt cgctcaaatt 2821 ctcgatagca ggatgaatac caaatatgac gagaacgaca agctgatcag agaggtcaag 2881 gtcatcacac tcaagtccaa gctcgtgagc gacttcagaa aagatttcca attttataaa 2941 gtcagggaga tcaacaatta ccaccacgct cacgacgctt atctcaacgc tgtcgtggga 3001 accgccctga tcaaaaaata ccccaagctg gaaagcgagt tcgtgtatgg cgattataaa 3061 gtgtacgacg tgaggaagat gatcgctaaa agcgagcagg aaatcggcaa ggctacagcc 3121 aagtactttt tctactccaa cattatgaac ttcttcaaga ccgagattac cctcgccaac 3181 ggcgaaatta ggaagaggcc cctgattgaa acaaatggag aaacaggcga aatcgtctgg 3241 gacaagggca gggacttcgc cacagtcaga aaagtgctgt ccatgcctca agtcaacatc 3301 gtcaaaaaga ccgaggtgca gaccggcggc tttagcaaag aaagcatcct gcccaagaga 3361 aactccgaca agctcatcgc taggaagaag gactgggacc ctaagaaata cggaggattt 3421 gactccccta ccgtcgccta ttccgtcctc gtcgtcgcta aggtggagaa gggcaagagc 3481 aagaagctca agagcgtcaa ggagctgctg ggaatcacca tcatggagag gagctccttc 3541 gaaaaaaacc ctattgattt cctggaggcc aagggctaca aggaggtcaa gaaggacctc 3601 atcatcaagc tgcccaaata cagcctcttc gaactggaaa atggcaggaa gagaatgctc 3661 gctagggccg gcgagctcca gaaaggaaat gagctggctc tgcccagcaa gtacgtcaac 3721 ttcctctatc tcgccagcca ctatgaaaag ctcaagggca gccccgaaga caatgagcag 3781 aagcagctct tcgtcgagca gcacaagcac tacctcgatg aaatcatcga gcaaatcagc 3841 gagttttcca aaagggtgat tctcgccgac gctaacctcg ataaggtcct ctccgcttac 3901 aacaagcata gagacaagcc catcagagaa caggccgaga acatcatcca cctgtttaca 3961 ctcacaaacc tcggagcccc tgccgctttt aaatacttcg atacaaccat tgataggaag 4021 aggtacacct ccaccaagga ggtgctggat gctaccctga ttcatcaatc catcacagga 4081 ctctacgaaa caaggattga cctgtcccaa ctgggaggcg acccgaaaaa gaaacgcaaa 4141 gttgggcgcg ccgacgcgct ggacgatttc gatctcgaca tgctgggttc tgatgccctc 4201 gatgactttg acctggatat gttgggaagc gacgcattgg atgactttga tctggacatg 4261 ctcggctccg atgctctgga cgatttcgat ctcgatatgt taattaacta cccgtacgac 4321 gttccggact acgcttctga ctacaaggac gacgacgaca aataataa //