BBa_K1983005
1
BBa_K1983005
gp45 phenylalanine mutant M25 with C-terminal 6XHis-Tag
2016-09-14T11:00:00Z
2016-10-16T05:08:20Z
T4 phage
T4 phage clamp protein gp45 with 6 stable mutations to phenylalanine.
false
false
_2450_
31949
31949
9
false
Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.
false
Vykintas Jauni??kis
annotation2483477
1
C-terminal 6XHis-Tag
range2483477
1
688
711
annotation2483478
1
Double translation stop codon
range2483478
1
712
714
annotation2483476
1
gp45m25
range2483476
1
4
687
BBa_K1983005_sequence
1
catatgaaactgtctaaagatactactgctttccttaaaaatttcgctactattaactctggtattttctttaaatccggtcaattcattttcactcgcgcagttaatggtacaacttttgcggaagcaaatatttctgacgttttcgattttgatgtagcattctttgatttgaacggttttttcggtattttctctttagttaatgatgatgcagaaatttcccagtcagaagatggaaatattaaaattgctgatgcccgctcaacaattttttggccagcagccgatccgagtacagtagttgctcctaataaaccattcccattcccggtagcatctgctgttactgaaattaaagctgaagactttcaacagctgtttcgtgtatctcgtggtctgcaaattgatacaattgctttcacggtaaaagaaggtaaattcgtaattaacggttttaataaagtagaagattctgctctgacccgtgttaaatattcttttactcttggtgatttcgatggtgaaaatacattcaatttcattatcaattttgcaaatttcaaaatgcaaccaggcaatttcaaacttttcttcttcgcaaaaggtaaacaaggtgctgctaaattcgaaggtgaacacgcgaatttcgtggtagctttcgaagctgattctacccacgatttcctcgagcatcaccaccaccatcactgatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z