BBa_K1985005
1
BBa_K1985005
mamX his-tagged, signal sequence cleaved
2016-10-13T11:00:00Z
2016-10-19T02:27:29Z
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.
Usage: The mam gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the protein is exposed to into in solution. This sequence produces a soluble protein because it has the membrane anchor cleaved and is targeted to the secretory pathway, which will leave the protein in the periplasm. It was expressed, purified and then exposed to iron.
Biology:
Mam is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
false
false
_2452_
29337
29337
9
false
Codon Optimised
false
Jasmine Cornish
annotation2522514
1
SecS-sol-mamX
range2522514
1
15
794
BBa_K1985005_sequence
1
aggaggaattacatatggctaagtcactgttcagggcgctggtcgccctgtcttttcttgcgccactgtggctcaacgccgcgccgcgcgtcatcacgctttctcccgccgagctcgcgcctcccattgtcgctgggatggccgccccccatcgcgatggccgcgagaagatggtctgctccagctgccatatcgtcacccccgcttccgccgctaccggtccgggcgcgggcacgctgcccatcgttgagggaaccccggcgccgcatgtggatggacgcgagaagatggcctgcgccagttgccacaccatcgtgaagaagggcagcgttgccaagagcggcaaggcaagccccgctccggtggcattctcgcagggcatgcccttacccgaggcgatgagcgtggcgctggcggttaccccggcaccggcgcccctgggcaacgaagcgcatgagcggatggtgcccttccgctatcaaggcaagatcgtcagcgtggcgggcgctggcacccgctcggtctggggtgatatctatattcagatcaacgatgggatcaatcctcccatgtggatcgaccttgccccgctctggttcctgcaagcggagggctgcttggtccgtcccggtatgttcgtcaagggcaccgccttccgcgacccgacccaggcgagcgccgggctggattacgccatgagcgtcatggccaatggcgaagtctgtgccctgcgtgacgaccatctcaacggcctgtgggctaatgttggaggcgtggatgccgaagagcggtaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z