BBa_K1985000
1
BBa_K1985000
mamP
2016-10-12T11:00:00Z
2016-10-19T01:32:45Z
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.
Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to iron in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
Biology:
MamP is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
false
false
_2452_
29337
29337
9
false
Codon Optimised
false
Jasmine Cornish
annotation2522185
1
mamP
range2522185
1
15
827
BBa_K1985006
1
BBa_K1985006
mamO
2016-10-13T11:00:00Z
2016-10-19T02:32:22Z
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.
MamO is involved in crystal nucleation.
Biology:
MamO is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
false
false
_2452_
29337
29337
9
false
Codon Optimised
false
Jasmine Cornish
annotation2522552
1
mamO
range2522552
1
15
1913
BBa_K1985001
1
BBa_K1985001
mamT
2016-10-13T11:00:00Z
2016-10-19T01:43:32Z
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.
Usage: The mamT gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to iron in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
Biology:
MamT is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
false
false
_2452_
29337
29337
9
false
Codon Optimised
false
Jasmine Cornish
annotation2522223
1
mamT
range2522223
1
15
539
BBa_K1321333
1
BBa_K1321333
pSB-AraC-pBAD
2014-10-07T11:00:00Z
2015-06-17T12:27:41Z
This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
false
false
_1696_
4206
21121
9
In stock
false
false
Laura de Arroyo Garcia
annotation2429239
1
pBAD
range2429239
1
1116
1210
annotation2429237
1
AraC
range2429237
1
1
1041
annotation2429238
1
PC
range2429238
1
1042
1115
BBa_K1985002
1
BBa_K1985002
mamX
2016-10-13T11:00:00Z
2016-10-19T01:45:03Z
The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.
Usage: The mamX gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to into in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
Biology:
MamX is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
false
false
_2452_
29337
29337
9
false
Codon Optimised
false
Jasmine Cornish
annotation2522235
1
mamX
range2522235
1
15
824
BBa_K1985009
1
BBa_K1985009
AraC pBAD mamOPXT
2016-10-13T11:00:00Z
2016-10-20T07:35:03Z
BBa_K1321333, BBa_K1985006, BBa_K1985000, BBa_K1985001 and BBa_K1985002
AraC pBAD mamOPTX
false
false
_2452_
30560
29337
9
false
Codon optimised
false
Braulio Carrillo-Sanchez, Jasmine Cornish
component2514411
1
BBa_K1985001
component2514409
1
BBa_K1985000
component2514410
1
BBa_K1985002
component2514408
1
BBa_K1985006
component2514407
1
BBa_K1321333
annotation2514410
1
BBa_K1985002
range2514410
1
3938
4747
annotation2514408
1
BBa_K1985006
range2514408
1
1217
3115
annotation2514409
1
BBa_K1985000
range2514409
1
3122
3931
annotation2514411
1
BBa_K1985001
range2514411
1
4754
5278
annotation2514407
1
BBa_K1321333
range2514407
1
1
1210
BBa_K1985002_sequence
1
aggaggaattacatatgaacaccaaagccgttgcacatcccgacatcgccgtctggatcatggcgttgggcattgccttcagcatggccctagttctgaccgcgctgttcaatgccaatccgtgggaagatcacacctacgatctggcgcctcccattgtcgctgggatggccgccccccatcgcgatggccgcgagaagatggtctgctccagctgccatatcgtcacccccgcttccgccgctaccggtccgggcgcgggcacgctgcccatcgttgagggaaccccggcgccgcatgtggatggacgcgagaagatggcctgcgccagttgccacaccatcgtgaagaagggcagcgttgccaagagcggcaaggcaagccccgctccggtggcattctcgcagggcatgcccttacccgaggcgatgagcgtggcgctggcggttaccccggcaccggcgcccctgggcaacgaagcgcatgagcggatggtgcccttccgctatcaaggcaagatcgtcagcgtggcgggcgctggcacccgctcggtctggggtgatatctatattcagatcaacgatgggatcaatcctcccatgtggatcgaccttgccccgctctggttcctgcaagcggagggctgcttggtccgtcccggtatgttcgtcaagggcaccgccttccgcgacccgacccaggcgagcgccgggctggattacgccatgagcgtcatggccaatggcgaagtctgtgccctgcgtgacgaccatctcaacggcctgtgggctaatgttggaggcgtggatgccgaagagcggtaatga
BBa_K1985000_sequence
1
aggaggaattacatatgaatagcaaactcgtcctgctggtggtgggtgtcgtttttgcactggtgcttgtcattgggcggcaggggggcgtcgttgctcctcagtcgatcagcgtttcgcctcagatgtcgactgcggctccggtcgctgccccgattgcttttccgcaggcgaccaatgtggccatggcggtggagcccatggccgcggcgggtggtaccgtggctgcaatggagtcgcccttgcccaatttcgttccgcgcaatctcaaggtctttgaaggccattggcagggcatggacggacgtctgatgaccgaggaactggcccgaaagttgaactatcctcgtggagtgcagggcgttctgttgggcgaggttactttgaacgcggcgttcagcggtctgttggggggggatgtcgtcgttcggattgatgacactccagtcaccaatatggaaaatttccaggcagctacccgcaatgtggcgaaccggtccgaggcgcggatcagcgtgatccgtaaggacaaccgtcccggcaccccggtcttgcgcaaactgaccgtggttttgcgcgcggccgaaggcggtttgggctttgcccagttggaaggcgcccccatgatccttccgggcgatcctcggccccatggatatcgcggcgcctgcaccgattgccatccggtaggccagggattcgaattgtcgcccgatcctgatctcatcagcctgccgccttcggccataacgcgtgatgcggttacccggggcgtcagtccccacgaagttcgtggcccgtgcgaagcttgccacgtgataaattaatga
BBa_K1985006_sequence
1
aggaggaattacatatgattgaaattggcgagaccatgggtgatcagcccaccaacaaaatcgtcttttgcgagcggtcgtggaaagcgcctgtctccatcctggcgttcctgatcctagtgactttcgcctggggggcctatctcctcgacaactacgacgaggacgactacttccgtggtagcgacgatatgtcggtcggccaattcctggtccgcaacgtcgccatgcctgatgtgcagcggctgtactatacagtaccaccggcggtggtcggtgtggggggcggcggcgtcaatgccggtcccgtcgcttcgggtgccatcgttggcgccaatggctatgtcattacgaccctgcattccgtcgccaatgtgccggacatcacggtccaggttgccacctctgctggaatccgtcggtttcccgcccaggtggtcaagaccattccgggccataatctcgcgctgctgaagttgcagacgaccgagaagttcttgcatttccgcatggcaaacattcaaaccgtggtccccggccagcaggttttcgccttcggtcggaacatggccggtgcgcctctggtacggcagggcatggtgcaatcctccgatgctccgctggcggtggggaccactcagatcacccatctgctgcgttccgacgcggtctatagctgggagcagaccggcggtcctctggttaatgctcagggtgatctggtaggtatcaacattgccgcaaccggtcccaccggcaaggtcgaaggctttaccgttcctgctcaggtgatcgtctcccatcttcaggacgtcgtgcgattcaagaccggtggcgccgcgggagttgctcctcccgcggcccaaactgtggccatgggatcctctagctggtggtctaaggccaaagcggtggtcggagggccgaccgccgtaccgggaatgggcatgaatgtggtgcaagggacggtgacgaccgggattccctcgggcatgccgtttgtcgataccgaccatgtgggaggggccaagatcggcggatattccatcgccgatattctcggcctaggcatgcttgccctggcggccggtgtcacgggtggcatgatgaccatgggcggcggagtgcttcaggtcgctggcatgatggtcttcttcggctatggcatgtatctgatccggccggtggtcttcctgaccaatgtcgtggtctatggcgcagcggcgcttcgcaatgataaggcccaactggttcagtgggacaaggtgaaaccgctgatcccttggggcgtcgctggcgtggtcatcggttatttcatcggtaacgccatcggagattccgtggtgggcgtgctgcttggtctcttcgccctgatcatggcaggcaaggcggtgttggaaattctgcaacccaatgcgggggaggatacggctgaagccattgctgccgccgaggctggcgatgagatggacgaactgatggctctggcggaaggaacaaccaggcccaagaccagcggcattgctttgccagagggtccaacccgttcagcggtactggggctgcccatgggcctgttcagcggcattctcggtatcagcggcggcgtcatcgaggttccgttgcagcgttatattgggcggatcagtctgcaaaacgccattgccaacagttcggtcctggtgttctgggcctcggtcgccggttcagtggtagcctttattcatggcggcagcaccggtcttattcattgggaggctccggtaacattggcgctggtgatgatcccaggtgcttatgttggcggcattctcggcgctcggttgatgcgggtgttgcctgtccgggtgcttaagggtatctatgcggcaaccatggccgctatcgccatcaagatgctgacaacggtgtaatga
BBa_K1985001_sequence
1
aggaggaattacatatgggtacgccagggggcggccgtcgctggatgaccttgatctcgatcaccttgctgatggtggtcggactgggactctattgggatgagctgtccctctccgccggcatctcccccgccacatcgccccgtcgggcggaggggcttttgttggggcggctgcccttgcccatggagccttcgattctgtcgccgctggagcatctcattgagccgccgcttcagtacaagctgatgaccattcgtcatatcccgccggtaatgccggggacaggcatgccccatccctatgtgggggattgcatccaatgccatctgatggtcggtggccccgctgccggatcacagttcaagacgccctatggcgccgtactggaaaacctgtcgcgggtccgcaaactggggcctcccattcttcccacgacgcgccagccgcatccgcctgccgggcgctgcattaagtgccatgacattgtggtcaaggtgcctgtggaaaagaagtccggcattaaatggctgttgtaatga
BBa_K1985009_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagatgattgaaattggcgagaccatgggtgatcagcccaccaacaaaatcgtcttttgcgagcggtcgtggaaagcgcctgtctccatcctggcgttcctgatcctagtgactttcgcctggggggcctatctcctcgacaactacgacgaggacgactacttccgtggtagcgacgatatgtcggtcggccaattcctggtccgcaacgtcgccatgcctgatgtgcagcggctgtactatacagtaccaccggcggtggtcggtgtggggggcggcggcgtcaatgccggtcccgtcgcttcgggtgccatcgttggcgccaatggctatgtcattacgaccctgcattccgtcgccaatgtgccggacatcacggtccaggttgccacctctgctggaatccgtcggtttcccgcccaggtggtcaagaccattccgggccataatctcgcgctgctgaagttgcagacgaccgagaagttcttgcatttccgcatggcaaacattcaaaccgtggtccccggccagcaggttttcgccttcggtcggaacatggccggtgcgcctctggtacggcagggcatggtgcaatcctccgatgctccgctggcggtggggaccactcagatcacccatctgctgcgttccgacgcggtctatagctgggagcagaccggcggtcctctggttaatgctcagggtgatctggtaggtatcaacattgccgcaaccggtcccaccggcaaggtcgaaggctttaccgttcctgctcaggtgatcgtctcccatcttcaggacgtcgtgcgattcaagaccggtggcgccgcgggagttgctcctcccgcggcccaaactgtggccatgggatcctctagctggtggtctaaggccaaagcggtggtcggagggccgaccgccgtaccgggaatgggcatgaatgtggtgcaagggacggtgacgaccgggattccctcgggcatgccgtttgtcgataccgaccatgtgggaggggccaagatcggcggatattccatcgccgatattctcggcctaggcatgcttgccctggcggccggtgtcacgggtggcatgatgaccatgggcggcggagtgcttcaggtcgctggcatgatggtcttcttcggctatggcatgtatctgatccggccggtggtcttcctgaccaatgtcgtggtctatggcgcagcggcgcttcgcaatgataaggcccaactggttcagtgggacaaggtgaaaccgctgatcccttggggcgtcgctggcgtggtcatcggttatttcatcggtaacgccatcggagattccgtggtgggcgtgctgcttggtctcttcgccctgatcatggcaggcaaggcggtgttggaaattctgcaacccaatgcgggggaggatacggctgaagccattgctgccgccgaggctggcgatgagatggacgaactgatggctctggcggaaggaacaaccaggcccaagaccagcggcattgctttgccagagggtccaacccgttcagcggtactggggctgcccatgggcctgttcagcggcattctcggtatcagcggcggcgtcatcgaggttccgttgcagcgttatattgggcggatcagtctgcaaaacgccattgccaacagttcggtcctggtgttctgggcctcggtcgccggttcagtggtagcctttattcatggcggcagcaccggtcttattcattgggaggctccggtaacattggcgctggtgatgatcccaggtgcttatgttggcggcattctcggcgctcggttgatgcgggtgttgcctgtccgggtgcttaagggtatctatgcggcaaccatggccgctatcgccatcaagatgctgacaacggtgtaatactagatgaatagcaaactcgtcctgctggtggtgggtgtcgtttttgcactggtgcttgtcattgggcggcaggggggcgtcgttgctcctcagtcgatcagcgtttcgcctcagatgtcgactgcggctccggtcgctgccccgattgcttttccgcaggcgaccaatgtggccatggcggtggagcccatggccgcggcgggtggtaccgtggctgcaatggagtcgcccttgcccaatttcgttccgcgcaatctcaaggtctttgaaggccattggcagggcatggacggacgtctgatgaccgaggaactggcccgaaagttgaactatcctcgtggagtgcagggcgttctgttgggcgaggttactttgaacgcggcgttcagcggtctgttggggggggatgtcgtcgttcggattgatgacactccagtcaccaatatggaaaatttccaggcagctacccgcaatgtggcgaaccggtccgaggcgcggatcagcgtgatccgtaaggacaaccgtcccggcaccccggtcttgcgcaaactgaccgtggttttgcgcgcggccgaaggcggtttgggctttgcccagttggaaggcgcccccatgatccttccgggcgatcctcggccccatggatatcgcggcgcctgcaccgattgccatccggtaggccagggattcgaattgtcgcccgatcctgatctcatcagcctgccgccttcggccataacgcgtgatgcggttacccggggcgtcagtccccacgaagttcgtggcccgtgcgaagcttgccacgtgataaattactagatgaacaccaaagccgttgcacatcccgacatcgccgtctggatcatggcgttgggcattgccttcagcatggccctagttctgaccgcgctgttcaatgccaatccgtgggaagatcacacctacgatctggcgcctcccattgtcgctgggatggccgccccccatcgcgatggccgcgagaagatggtctgctccagctgccatatcgtcacccccgcttccgccgctaccggtccgggcgcgggcacgctgcccatcgttgagggaaccccggcgccgcatgtggatggacgcgagaagatggcctgcgccagttgccacaccatcgtgaagaagggcagcgttgccaagagcggcaaggcaagccccgctccggtggcattctcgcagggcatgcccttacccgaggcgatgagcgtggcgctggcggttaccccggcaccggcgcccctgggcaacgaagcgcatgagcggatggtgcccttccgctatcaaggcaagatcgtcagcgtggcgggcgctggcacccgctcggtctggggtgatatctatattcagatcaacgatgggatcaatcctcccatgtggatcgaccttgccccgctctggttcctgcaagcggagggctgcttggtccgtcccggtatgttcgtcaagggcaccgccttccgcgacccgacccaggcgagcgccgggctggattacgccatgagcgtcatggccaatggcgaagtctgtgccctgcgtgacgaccatctcaacggcctgtgggctaatgttggaggcgtggatgccgaagagcggtaatactagatgggtacgccagggggcggccgtcgctggatgaccttgatctcgatcaccttgctgatggtggtcggactgggactctattgggatgagctgtccctctccgccggcatctcccccgccacatcgccccgtcgggcggaggggcttttgttggggcggctgcccttgcccatggagccttcgattctgtcgccgctggagcatctcattgagccgccgcttcagtacaagctgatgaccattcgtcatatcccgccggtaatgccggggacaggcatgccccatccctatgtgggggattgcatccaatgccatctgatggtcggtggccccgctgccggatcacagttcaagacgccctatggcgccgtactggaaaacctgtcgcgggtccgcaaactggggcctcccattcttcccacgacgcgccagccgcatccgcctgccgggcgctgcattaagtgccatgacattgtggtcaaggtgcctgtggaaaagaagtccggcattaaatggctgttgtaa
BBa_K1321333_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z