BBa_K1985013 1 BBa_K1985013 Sequence coding for Sup35 with residues 1-61 with an arabinose inducible promoter 2016-10-13T11:00:00Z 2016-10-19T02:52:46Z Coding sequence synthesised by IDT. This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. This part contains three segments, the CsgA signal sequence, Sup35NM and a prion forming domain. Our improved BioBrick aims to optimize the aggregation process of amyloid fibrils with the addition of a short and specific prion forming domain of 61 aminoacid residues. This was inserted into the pSB1A3 backbone and uses the promoter BBa_K1321333. false false _2452_ 29437 29437 9 No part sequence false Codon optimised. false Rita Adriani component2506955 1 BBa_K1985010 component2506954 1 BBa_K1321333 annotation2506954 1 BBa_K1321333 range2506954 1 1 1210 annotation2506955 1 BBa_K1985010 range2506955 1 1219 1227 BBa_K1985010 1 BBa_K1985010 Sequence coding for amyloid Sup35 residues 1-61 2016-10-13T11:00:00Z 2016-10-25T11:27:16Z Coding genome synthesised by IDT. This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. This part contains three segments, the CsgA signal sequence, Sup35NM and a prion forming domain. Our improved BioBrick aims to optimize the aggregation process of amyloid fibrils with the addition of a short and specific prion forming domain of 61 aminoacid residues. This was inserted into the pSB1C3 backbone. false false _2452_ 29437 29437 9 false Making sure the part was compatible with pSB1C3 vector. false Rita Adriani annotation2530120 1 CsgA-SS_Sup35-1-61 range2530120 1 1 338 BBa_K1321333 1 BBa_K1321333 pSB-AraC-pBAD 2014-10-07T11:00:00Z 2015-06-17T12:27:41Z This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter) This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream. false false _1696_ 4206 21121 9 In stock false false Laura de Arroyo Garcia annotation2429239 1 pBAD range2429239 1 1116 1210 annotation2429237 1 AraC range2429237 1 1 1041 annotation2429238 1 PC range2429238 1 1042 1115 BBa_K1985010_sequence 1 aggaggaattacatatgaaactgcttaaagtagcagcgatcgcggcaattgtgttcagtggaagcgcgctggctggtgttgtacctcagtatggtggaggcggcaaccacggcggcggaggcaataattcagggccgaatgccgcggcgatgtcggattcaaaccagggcaacaaccagcaaaattaccagcagtattcccaaaacggcaaccagcaacaaggtaacaatcgctatcaggggtaccaggcgtacaatgcccaagcacaaccggcaggcggctattatcagaactaccagggctacagcggttaccaacagggtggttatcagtaatga BBa_K1985013_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaaaaaaaa BBa_K1321333_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z