BBa_K1985014 1 BBa_K1985014 Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter 2016-10-13T11:00:00Z 2016-10-19T03:22:16Z Coding sequence synthesised by IDT. This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 4 segments, the CsgA signal sequence, Sup35NM, prion forming domain of 61 residues and the electron transfer protein cytochrome 562. false false _2452_ 29437 29437 9 No part sequence false Codon optimised. false Rita Adriani component2507041 1 BBa_K1985011 component2507040 1 BBa_K1321333 annotation2507040 1 BBa_K1321333 range2507040 1 1 1210 annotation2507041 1 BBa_K1985011 range2507041 1 1219 1232 BBa_K1985011 1 BBa_K1985011 Sequence coding for amyloid Sup35 residues 1-61 and Cytochrome b562 2016-10-13T11:00:00Z 2016-10-25T11:42:23Z Includes the coding sequence which was synthesised by IDT. This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 4 segments, the CsgA signal sequence, Sup35NM, prion forming domain of 61 residues and the electron transfer protein cytochrome 562. false false _2452_ 29437 29437 9 false Making sure it was compatible with pSB1C3 vector. false Rita Adriani annotation2530122 1 CsgA-SS_sup35-1-61_cytb562 range2530122 1 1 686 BBa_K1321333 1 BBa_K1321333 pSB-AraC-pBAD 2014-10-07T11:00:00Z 2015-06-17T12:27:41Z This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter) This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream. false false _1696_ 4206 21121 9 In stock false false Laura de Arroyo Garcia annotation2429238 1 PC range2429238 1 1042 1115 annotation2429237 1 AraC range2429237 1 1 1041 annotation2429239 1 pBAD range2429239 1 1116 1210 BBa_K1985014_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagaggggggggccccccc BBa_K1985011_sequence 1 aggaggaattacatatgaaactgcttaaagtagcagcgatcgcggcaattgtgttcagtggaagcgcgctggctggtgttgtacctcagtatggtggaggcggcaaccacggcggcggaggcaataattcagggccgaatgccgcggcgatgtcggattcaaaccagggcaacaaccagcaaaattaccagcagtattcccaaaacggcaaccagcaacaaggtaacaatcgctatcaggggtaccaggcgtacaatgcccaagcacaaccggcaggcggctattatcagaactaccagggctacagcggttaccaacagggtggttatcagggtggcggttcaggcggtggcggttcaggcgcggacttagaagataatatggaaactttgaacgacaacctgaaagtgattgagaaggctgataacgccgcccaagtgaaggatgcactgaccaagatgcgtgctgccgcgctggacgcccagaaagcgaccccaccgaaattagaggacaagtccccggatagccctgaaatgaaagattttcgccatggttttgacatcttggtgggccagattgacgacgctttaaagctggcaaatgaaggcaaggtgaaagaagcacaagccgcagccgagcaattaaaaacgacccgtaacgcataccaccagaaatatcgctaatga BBa_K1321333_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z