BBa_K1985015 1 BBa_K1985015 pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM] 2016-10-13T11:00:00Z 2016-10-20T12:04:46Z Coding sequence synthesized by IDT. This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 2 segments, the CsgA signal sequence and Sup35NM false false _2452_ 29437 29437 9 false Codon optimised. false Hanya Benotmane component2507089 1 BBa_K1985012 component2507088 1 BBa_K1321333 annotation2507088 1 BBa_K1321333 range2507088 1 1 1210 annotation2507089 1 BBa_K1985012 range2507089 1 1219 1229 BBa_K1985012 1 BBa_K1985012 [CsgA-SS-Sup35NM] 2016-10-13T11:00:00Z 2016-10-25T03:51:08Z Coding sequence synthesized by IDT. Sup35NM is a prion protein. false false _2452_ 29437 29437 9 false Codon optimised. false Hanya Benotmane annotation2530171 1 CsgA-SS_Sup35NM range2530171 1 1 932 BBa_K1321333 1 BBa_K1321333 pSB-AraC-pBAD 2014-10-07T11:00:00Z 2015-06-17T12:27:41Z This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter) This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream. false false _1696_ 4206 21121 9 In stock false false Laura de Arroyo Garcia annotation2429239 1 pBAD range2429239 1 1116 1210 annotation2429237 1 AraC range2429237 1 1 1041 annotation2429238 1 PC range2429238 1 1042 1115 BBa_K1985015_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagttttttttttt BBa_K1985012_sequence 1 aggaggaattacatatgaaactgcttaaagtagcagcgatcgcggcaattgtgttcagtggaagcgcgctggctggtgttgtacctcagtatggtggaggcggcaaccacggcggcggaggcaataattcagggccgaatgccgcggcgatgtcggattcaaaccagggcaacaaccagcaaaattaccagcagtattcccaaaacggcaaccagcaacaaggtaacaatcgctatcaggggtaccaggcgtacaatgcccaagcacaaccggcaggcggctattatcagaactaccagggctacagcggttaccaacagggtggttatcagcagtataaccccgatgctgggtatcagcagcagtataatccccaaggcgggtatcagcagtacaacccacagggtggctatcagcaacagtttaatccgcagggcggacgcggtaactacaaaaacttcaactataacaataatttacaggggtaccaagctggttttcagcctcagagtcaggggatgtctctgaatgatttccaaaaacagcaaaagcaagcagctccaaaaccgaaaaaaaccctgaaattagtgtccagctcgggaattaaactcgctaacgccacgaagaaggtgggcacgaaaccggctgaaagcgataaaaaagaggaagagaagagcgcggaaaccaaggaaccgaccaaagagccgactaaagtcgaagaacctgtgaaaaaagaagagaaacctgtacagacggaagagaaaactgaggagaaatcggaattacccaaagtcgaggatttgaaaatttcggaaagcacccataacacaaacaatgcgaatgttacatccgcggatgcgttgattaaagaacaggaagaggaagttgacgatgaggtggtgaatgatcaccatcatcatcatcattaatga BBa_K1321333_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z