BBa_K1985015
1
BBa_K1985015
pSB1A3-[AraC-pBAD]-[CsgA-SS-Sup35NM]
2016-10-13T11:00:00Z
2016-10-20T12:04:46Z
Coding sequence synthesized by IDT.
This part is an improved version of the Envirowire fusion protein(BBa_K1739003). It contains 2 segments, the CsgA signal sequence and Sup35NM
false
false
_2452_
29437
29437
9
false
Codon optimised.
false
Hanya Benotmane
component2507089
1
BBa_K1985012
component2507088
1
BBa_K1321333
annotation2507088
1
BBa_K1321333
range2507088
1
1
1210
annotation2507089
1
BBa_K1985012
range2507089
1
1219
1229
BBa_K1985012
1
BBa_K1985012
[CsgA-SS-Sup35NM]
2016-10-13T11:00:00Z
2016-10-25T03:51:08Z
Coding sequence synthesized by IDT.
Sup35NM is a prion protein.
false
false
_2452_
29437
29437
9
false
Codon optimised.
false
Hanya Benotmane
annotation2530171
1
CsgA-SS_Sup35NM
range2530171
1
1
932
BBa_K1321333
1
BBa_K1321333
pSB-AraC-pBAD
2014-10-07T11:00:00Z
2015-06-17T12:27:41Z
This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
false
false
_1696_
4206
21121
9
In stock
false
false
Laura de Arroyo Garcia
annotation2429239
1
pBAD
range2429239
1
1116
1210
annotation2429237
1
AraC
range2429237
1
1
1041
annotation2429238
1
PC
range2429238
1
1042
1115
BBa_K1985015_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagttttttttttt
BBa_K1985012_sequence
1
aggaggaattacatatgaaactgcttaaagtagcagcgatcgcggcaattgtgttcagtggaagcgcgctggctggtgttgtacctcagtatggtggaggcggcaaccacggcggcggaggcaataattcagggccgaatgccgcggcgatgtcggattcaaaccagggcaacaaccagcaaaattaccagcagtattcccaaaacggcaaccagcaacaaggtaacaatcgctatcaggggtaccaggcgtacaatgcccaagcacaaccggcaggcggctattatcagaactaccagggctacagcggttaccaacagggtggttatcagcagtataaccccgatgctgggtatcagcagcagtataatccccaaggcgggtatcagcagtacaacccacagggtggctatcagcaacagtttaatccgcagggcggacgcggtaactacaaaaacttcaactataacaataatttacaggggtaccaagctggttttcagcctcagagtcaggggatgtctctgaatgatttccaaaaacagcaaaagcaagcagctccaaaaccgaaaaaaaccctgaaattagtgtccagctcgggaattaaactcgctaacgccacgaagaaggtgggcacgaaaccggctgaaagcgataaaaaagaggaagagaagagcgcggaaaccaaggaaccgaccaaagagccgactaaagtcgaagaacctgtgaaaaaagaagagaaacctgtacagacggaagagaaaactgaggagaaatcggaattacccaaagtcgaggatttgaaaatttcggaaagcacccataacacaaacaatgcgaatgttacatccgcggatgcgttgattaaagaacaggaagaggaagttgacgatgaggtggtgaatgatcaccatcatcatcatcattaatga
BBa_K1321333_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z