BBa_K1321333 1 BBa_K1321333 pSB-AraC-pBAD 2014-10-07T11:00:00Z 2015-06-17T12:27:41Z This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter) This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream. false false _1696_ 4206 21121 9 In stock false false Laura de Arroyo Garcia annotation2429238 1 PC range2429238 1 1042 1115 annotation2429237 1 AraC range2429237 1 1 1041 annotation2429239 1 pBAD range2429239 1 1116 1210 BBa_K1985006 1 BBa_K1985006 mamO 2016-10-13T11:00:00Z 2016-10-19T02:32:22Z The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent. MamO is involved in crystal nucleation. Biology: MamO is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome. false false _2452_ 29337 29337 9 false Codon Optimised false Jasmine Cornish annotation2522552 1 mamO range2522552 1 15 1913 BBa_K1985000 1 BBa_K1985000 mamP 2016-10-12T11:00:00Z 2016-10-19T01:32:45Z The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent. Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to iron in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression. Biology: MamP is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome. false false _2452_ 29337 29337 9 false Codon Optimised false Jasmine Cornish annotation2522185 1 mamP range2522185 1 15 827 BBa_K1985017 1 BBa_K1985017 AraC pBAD mamOP 2016-10-13T11:00:00Z 2016-10-14T10:06:08Z BBa_K1321333, BBa_K1985006 and BBa_K1985000 AraC pBAD mamOP false false _2452_ 29337 29337 9 false Magnetite formation false Jasmine Cornish component2506768 1 BBa_K1985000 component2506767 1 BBa_K1985006 component2506766 1 BBa_K1321333 annotation2506766 1 BBa_K1321333 range2506766 1 1 1210 annotation2506767 1 BBa_K1985006 range2506767 1 1217 3115 annotation2506768 1 BBa_K1985000 range2506768 1 3122 3931 BBa_K1985000_sequence 1 aggaggaattacatatgaatagcaaactcgtcctgctggtggtgggtgtcgtttttgcactggtgcttgtcattgggcggcaggggggcgtcgttgctcctcagtcgatcagcgtttcgcctcagatgtcgactgcggctccggtcgctgccccgattgcttttccgcaggcgaccaatgtggccatggcggtggagcccatggccgcggcgggtggtaccgtggctgcaatggagtcgcccttgcccaatttcgttccgcgcaatctcaaggtctttgaaggccattggcagggcatggacggacgtctgatgaccgaggaactggcccgaaagttgaactatcctcgtggagtgcagggcgttctgttgggcgaggttactttgaacgcggcgttcagcggtctgttggggggggatgtcgtcgttcggattgatgacactccagtcaccaatatggaaaatttccaggcagctacccgcaatgtggcgaaccggtccgaggcgcggatcagcgtgatccgtaaggacaaccgtcccggcaccccggtcttgcgcaaactgaccgtggttttgcgcgcggccgaaggcggtttgggctttgcccagttggaaggcgcccccatgatccttccgggcgatcctcggccccatggatatcgcggcgcctgcaccgattgccatccggtaggccagggattcgaattgtcgcccgatcctgatctcatcagcctgccgccttcggccataacgcgtgatgcggttacccggggcgtcagtccccacgaagttcgtggcccgtgcgaagcttgccacgtgataaattaatga BBa_K1985006_sequence 1 aggaggaattacatatgattgaaattggcgagaccatgggtgatcagcccaccaacaaaatcgtcttttgcgagcggtcgtggaaagcgcctgtctccatcctggcgttcctgatcctagtgactttcgcctggggggcctatctcctcgacaactacgacgaggacgactacttccgtggtagcgacgatatgtcggtcggccaattcctggtccgcaacgtcgccatgcctgatgtgcagcggctgtactatacagtaccaccggcggtggtcggtgtggggggcggcggcgtcaatgccggtcccgtcgcttcgggtgccatcgttggcgccaatggctatgtcattacgaccctgcattccgtcgccaatgtgccggacatcacggtccaggttgccacctctgctggaatccgtcggtttcccgcccaggtggtcaagaccattccgggccataatctcgcgctgctgaagttgcagacgaccgagaagttcttgcatttccgcatggcaaacattcaaaccgtggtccccggccagcaggttttcgccttcggtcggaacatggccggtgcgcctctggtacggcagggcatggtgcaatcctccgatgctccgctggcggtggggaccactcagatcacccatctgctgcgttccgacgcggtctatagctgggagcagaccggcggtcctctggttaatgctcagggtgatctggtaggtatcaacattgccgcaaccggtcccaccggcaaggtcgaaggctttaccgttcctgctcaggtgatcgtctcccatcttcaggacgtcgtgcgattcaagaccggtggcgccgcgggagttgctcctcccgcggcccaaactgtggccatgggatcctctagctggtggtctaaggccaaagcggtggtcggagggccgaccgccgtaccgggaatgggcatgaatgtggtgcaagggacggtgacgaccgggattccctcgggcatgccgtttgtcgataccgaccatgtgggaggggccaagatcggcggatattccatcgccgatattctcggcctaggcatgcttgccctggcggccggtgtcacgggtggcatgatgaccatgggcggcggagtgcttcaggtcgctggcatgatggtcttcttcggctatggcatgtatctgatccggccggtggtcttcctgaccaatgtcgtggtctatggcgcagcggcgcttcgcaatgataaggcccaactggttcagtgggacaaggtgaaaccgctgatcccttggggcgtcgctggcgtggtcatcggttatttcatcggtaacgccatcggagattccgtggtgggcgtgctgcttggtctcttcgccctgatcatggcaggcaaggcggtgttggaaattctgcaacccaatgcgggggaggatacggctgaagccattgctgccgccgaggctggcgatgagatggacgaactgatggctctggcggaaggaacaaccaggcccaagaccagcggcattgctttgccagagggtccaacccgttcagcggtactggggctgcccatgggcctgttcagcggcattctcggtatcagcggcggcgtcatcgaggttccgttgcagcgttatattgggcggatcagtctgcaaaacgccattgccaacagttcggtcctggtgttctgggcctcggtcgccggttcagtggtagcctttattcatggcggcagcaccggtcttattcattgggaggctccggtaacattggcgctggtgatgatcccaggtgcttatgttggcggcattctcggcgctcggttgatgcgggtgttgcctgtccgggtgcttaagggtatctatgcggcaaccatggccgctatcgccatcaagatgctgacaacggtgtaatga BBa_K1985017_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagatgattgaaattggcgagaccatgggtgatcagcccaccaacaaaatcgtcttttgcgagcggtcgtggaaagcgcctgtctccatcctggcgttcctgatcctagtgactttcgcctggggggcctatctcctcgacaactacgacgaggacgactacttccgtggtagcgacgatatgtcggtcggccaattcctggtccgcaacgtcgccatgcctgatgtgcagcggctgtactatacagtaccaccggcggtggtcggtgtggggggcggcggcgtcaatgccggtcccgtcgcttcgggtgccatcgttggcgccaatggctatgtcattacgaccctgcattccgtcgccaatgtgccggacatcacggtccaggttgccacctctgctggaatccgtcggtttcccgcccaggtggtcaagaccattccgggccataatctcgcgctgctgaagttgcagacgaccgagaagttcttgcatttccgcatggcaaacattcaaaccgtggtccccggccagcaggttttcgccttcggtcggaacatggccggtgcgcctctggtacggcagggcatggtgcaatcctccgatgctccgctggcggtggggaccactcagatcacccatctgctgcgttccgacgcggtctatagctgggagcagaccggcggtcctctggttaatgctcagggtgatctggtaggtatcaacattgccgcaaccggtcccaccggcaaggtcgaaggctttaccgttcctgctcaggtgatcgtctcccatcttcaggacgtcgtgcgattcaagaccggtggcgccgcgggagttgctcctcccgcggcccaaactgtggccatgggatcctctagctggtggtctaaggccaaagcggtggtcggagggccgaccgccgtaccgggaatgggcatgaatgtggtgcaagggacggtgacgaccgggattccctcgggcatgccgtttgtcgataccgaccatgtgggaggggccaagatcggcggatattccatcgccgatattctcggcctaggcatgcttgccctggcggccggtgtcacgggtggcatgatgaccatgggcggcggagtgcttcaggtcgctggcatgatggtcttcttcggctatggcatgtatctgatccggccggtggtcttcctgaccaatgtcgtggtctatggcgcagcggcgcttcgcaatgataaggcccaactggttcagtgggacaaggtgaaaccgctgatcccttggggcgtcgctggcgtggtcatcggttatttcatcggtaacgccatcggagattccgtggtgggcgtgctgcttggtctcttcgccctgatcatggcaggcaaggcggtgttggaaattctgcaacccaatgcgggggaggatacggctgaagccattgctgccgccgaggctggcgatgagatggacgaactgatggctctggcggaaggaacaaccaggcccaagaccagcggcattgctttgccagagggtccaacccgttcagcggtactggggctgcccatgggcctgttcagcggcattctcggtatcagcggcggcgtcatcgaggttccgttgcagcgttatattgggcggatcagtctgcaaaacgccattgccaacagttcggtcctggtgttctgggcctcggtcgccggttcagtggtagcctttattcatggcggcagcaccggtcttattcattgggaggctccggtaacattggcgctggtgatgatcccaggtgcttatgttggcggcattctcggcgctcggttgatgcgggtgttgcctgtccgggtgcttaagggtatctatgcggcaaccatggccgctatcgccatcaagatgctgacaacggtgtaatactagatgaatagcaaactcgtcctgctggtggtgggtgtcgtttttgcactggtgcttgtcattgggcggcaggggggcgtcgttgctcctcagtcgatcagcgtttcgcctcagatgtcgactgcggctccggtcgctgccccgattgcttttccgcaggcgaccaatgtggccatggcggtggagcccatggccgcggcgggtggtaccgtggctgcaatggagtcgcccttgcccaatttcgttccgcgcaatctcaaggtctttgaaggccattggcagggcatggacggacgtctgatgaccgaggaactggcccgaaagttgaactatcctcgtggagtgcagggcgttctgttgggcgaggttactttgaacgcggcgttcagcggtctgttggggggggatgtcgtcgttcggattgatgacactccagtcaccaatatggaaaatttccaggcagctacccgcaatgtggcgaaccggtccgaggcgcggatcagcgtgatccgtaaggacaaccgtcccggcaccccggtcttgcgcaaactgaccgtggttttgcgcgcggccgaaggcggtttgggctttgcccagttggaaggcgcccccatgatccttccgggcgatcctcggccccatggatatcgcggcgcctgcaccgattgccatccggtaggccagggattcgaattgtcgcccgatcctgatctcatcagcctgccgccttcggccataacgcgtgatgcggttacccggggcgtcagtccccacgaagttcgtggcccgtgcgaagcttgccacgtgataaat BBa_K1321333_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z