LOCUS       BBa_K1987000            1417 bp    DNA     linear       12-Jun-2024
DEFINITION  GFP with BsaI sites for promoter insertion
ACCESSION   BBa_K1987000
VERSION     BBa_K1987000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     promoter        229..358
                     /label=k206000
     RBS             367..378
                     /label=B0034
     CDS             385..1143
                     /label=K145015
     misc_feature    1100..1137
                     /label=LVA tag
     misc_feature    1152..1280
                     /label=B0015
     misc_feature    1032..1032
                     /label=BsaI fix 
     misc_feature    229..1280
                     /label=BBa_K1212006
     stem_loop       1300..1343
                     /label=stem_loop
     misc_feature    1289..1368
                     /label=BBa_B0010
     terminator      1289..1368
                     /label=BBa_B0010
     stem_loop       1384..1403
                     /label=T7 TE
     misc_feature    1410..1410
                     /label=stop
     polyA_site      1404..1417
                     /label=polya
     misc_feature    1377..1417
                     /label=BBa_B0012
     terminator      1377..1417
                     /label=BBa_B0012
     terminator      1289..1417
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagac attgattatt
      241 tgcacggcgt cacactttgc tatgccatag caagatagtc cataagatta gcggatccta
      301 cctgacgctt tttatcgcaa ctctctactg tttctccata ccgttttttt gggctagcta
      361 ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      421 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      481 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      541 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      601 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      661 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      721 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      781 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      841 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      901 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      961 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     1021 gaaaagagag atcacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     1081 atggatgaac tatacaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa
     1141 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1261 tgggcctttc tgcgtttata tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1321 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1381 actggctcac cttcgggtgg gcctttctgc gtttata
//